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Comparison Between K(3)EDTA and Lithium Heparin as Anticoagulant to Isolate Bovine Granulocytes From Blood
Granulocytes play a key role in the defense against invading pathogens. To study granulocyte functions, the isolation of a pure and active cell population from fresh blood is required. Anticoagulants and red blood cells (RBCs) lysis used in the isolation procedure may influence cell harvest, cell ma...
Autores principales: | Baien, Shima Hassan, Langer, Melissa Natalie, Heppelmann, Maike, von Köckritz-Blickwede, Maren, de Buhr, Nicole |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052352/ https://www.ncbi.nlm.nih.gov/pubmed/30050531 http://dx.doi.org/10.3389/fimmu.2018.01570 |
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