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SUMO1‐conjugation is altered during normal aging but not by increased amyloid burden
A proper equilibrium of post‐translational protein modifications is essential for normal cell physiology, and alteration in these processes is key in neurodegenerative disorders such as Alzheimer's disease. Recently, for instance, alteration in protein SUMOylation has been linked to amyloid pat...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052395/ https://www.ncbi.nlm.nih.gov/pubmed/29633471 http://dx.doi.org/10.1111/acel.12760 |
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author | Stankova, Trayana Piepkorn, Lars Bayer, Thomas A. Jahn, Olaf Tirard, Marilyn |
author_facet | Stankova, Trayana Piepkorn, Lars Bayer, Thomas A. Jahn, Olaf Tirard, Marilyn |
author_sort | Stankova, Trayana |
collection | PubMed |
description | A proper equilibrium of post‐translational protein modifications is essential for normal cell physiology, and alteration in these processes is key in neurodegenerative disorders such as Alzheimer's disease. Recently, for instance, alteration in protein SUMOylation has been linked to amyloid pathology. In this work, we aimed to elucidate the role of protein SUMOylation during aging and increased amyloid burden in vivo using a His(6)‐HA‐SUMO1 knock‐in mouse in the 5XFAD model of Alzheimer's disease. Interestingly, we did not observe any alteration in the levels of SUMO1‐conjugation related to Alzheimer's disease. SUMO1 conjugates remained localized to neuronal nuclei upon increased amyloid burden and during aging and were not detected in amyloid plaques. Surprisingly however, we observed age‐related alterations in global levels of SUMO1 conjugation and at the level of individual substrates using quantitative proteomic analysis. The identified SUMO1 candidate substrates are dominantly nuclear proteins, mainly involved in RNA processing. Our findings open novel directions of research for studying a functional link between SUMOylation and its role in guarding nuclear functions during aging. |
format | Online Article Text |
id | pubmed-6052395 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60523952018-08-01 SUMO1‐conjugation is altered during normal aging but not by increased amyloid burden Stankova, Trayana Piepkorn, Lars Bayer, Thomas A. Jahn, Olaf Tirard, Marilyn Aging Cell Original Articles A proper equilibrium of post‐translational protein modifications is essential for normal cell physiology, and alteration in these processes is key in neurodegenerative disorders such as Alzheimer's disease. Recently, for instance, alteration in protein SUMOylation has been linked to amyloid pathology. In this work, we aimed to elucidate the role of protein SUMOylation during aging and increased amyloid burden in vivo using a His(6)‐HA‐SUMO1 knock‐in mouse in the 5XFAD model of Alzheimer's disease. Interestingly, we did not observe any alteration in the levels of SUMO1‐conjugation related to Alzheimer's disease. SUMO1 conjugates remained localized to neuronal nuclei upon increased amyloid burden and during aging and were not detected in amyloid plaques. Surprisingly however, we observed age‐related alterations in global levels of SUMO1 conjugation and at the level of individual substrates using quantitative proteomic analysis. The identified SUMO1 candidate substrates are dominantly nuclear proteins, mainly involved in RNA processing. Our findings open novel directions of research for studying a functional link between SUMOylation and its role in guarding nuclear functions during aging. John Wiley and Sons Inc. 2018-04-06 2018-08 /pmc/articles/PMC6052395/ /pubmed/29633471 http://dx.doi.org/10.1111/acel.12760 Text en © 2018 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Stankova, Trayana Piepkorn, Lars Bayer, Thomas A. Jahn, Olaf Tirard, Marilyn SUMO1‐conjugation is altered during normal aging but not by increased amyloid burden |
title |
SUMO1‐conjugation is altered during normal aging but not by increased amyloid burden |
title_full |
SUMO1‐conjugation is altered during normal aging but not by increased amyloid burden |
title_fullStr |
SUMO1‐conjugation is altered during normal aging but not by increased amyloid burden |
title_full_unstemmed |
SUMO1‐conjugation is altered during normal aging but not by increased amyloid burden |
title_short |
SUMO1‐conjugation is altered during normal aging but not by increased amyloid burden |
title_sort | sumo1‐conjugation is altered during normal aging but not by increased amyloid burden |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052395/ https://www.ncbi.nlm.nih.gov/pubmed/29633471 http://dx.doi.org/10.1111/acel.12760 |
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