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Functional profiles of coronal and dentin caries in children
Background: Dental caries results from a dysbiosis of tooth-associated biofilms and frequently extends through enamel into dentin which has a different structure and composition. Objective: To evaluate the metatranscriptome of caries to determine the metabolic potential of caries communities compare...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052428/ https://www.ncbi.nlm.nih.gov/pubmed/30034639 http://dx.doi.org/10.1080/20002297.2018.1495976 |
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author | Kressirer, Christine A Chen, Tsute Lake Harriman, Kristie Frias-Lopez, Jorge Dewhirst, Floyd E Tavares, Mary A Tanner, Anne CR |
author_facet | Kressirer, Christine A Chen, Tsute Lake Harriman, Kristie Frias-Lopez, Jorge Dewhirst, Floyd E Tavares, Mary A Tanner, Anne CR |
author_sort | Kressirer, Christine A |
collection | PubMed |
description | Background: Dental caries results from a dysbiosis of tooth-associated biofilms and frequently extends through enamel into dentin which has a different structure and composition. Objective: To evaluate the metatranscriptome of caries to determine the metabolic potential of caries communities compared with health. Design: Samples from children, caries-free (CF: n = 4) or with coronal (CC: n = 5) or dentin (DC: n = 5) caries were examined for gene expression potential. Functional profiling was performed using HUMAnN2 (HMP Unified Metabolic Analysis Network). Results: There was increased gene expression diversity in DC compared with CC and CF. Genes in CF included alcohol dehydrogenase from Neisseria sicca, methylenetetrahydrofolate reductase from Streptococcus sanguinis and choline kinase from streptococci. Genes in CC mapped mainly to Streptococcus mutans. Arginine deiminase in DC mapped to S. sanguinis and Actinomyces naeslundii. Glycerol kinase genes mapped to S. sanguinis in all groups whereas glycerol kinase in DC were from Rothia, Prevotella and streptococci. Uracil-DNA glycosylase in DC mapped to Prevotella denticola and Actinomyces. Repressor LexA in DC mapped to Scardovia wiggsiae, Dialister invisus and Veillonella parvula. Conclusions: Functional profiling revealed enzyme activities in both caries and caries-free communities and clarified marked differences between coronal and dentin caries in bacterial composition and potential gene expression. |
format | Online Article Text |
id | pubmed-6052428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-60524282018-07-20 Functional profiles of coronal and dentin caries in children Kressirer, Christine A Chen, Tsute Lake Harriman, Kristie Frias-Lopez, Jorge Dewhirst, Floyd E Tavares, Mary A Tanner, Anne CR J Oral Microbiol Original Article Background: Dental caries results from a dysbiosis of tooth-associated biofilms and frequently extends through enamel into dentin which has a different structure and composition. Objective: To evaluate the metatranscriptome of caries to determine the metabolic potential of caries communities compared with health. Design: Samples from children, caries-free (CF: n = 4) or with coronal (CC: n = 5) or dentin (DC: n = 5) caries were examined for gene expression potential. Functional profiling was performed using HUMAnN2 (HMP Unified Metabolic Analysis Network). Results: There was increased gene expression diversity in DC compared with CC and CF. Genes in CF included alcohol dehydrogenase from Neisseria sicca, methylenetetrahydrofolate reductase from Streptococcus sanguinis and choline kinase from streptococci. Genes in CC mapped mainly to Streptococcus mutans. Arginine deiminase in DC mapped to S. sanguinis and Actinomyces naeslundii. Glycerol kinase genes mapped to S. sanguinis in all groups whereas glycerol kinase in DC were from Rothia, Prevotella and streptococci. Uracil-DNA glycosylase in DC mapped to Prevotella denticola and Actinomyces. Repressor LexA in DC mapped to Scardovia wiggsiae, Dialister invisus and Veillonella parvula. Conclusions: Functional profiling revealed enzyme activities in both caries and caries-free communities and clarified marked differences between coronal and dentin caries in bacterial composition and potential gene expression. Taylor & Francis 2018-07-16 /pmc/articles/PMC6052428/ /pubmed/30034639 http://dx.doi.org/10.1080/20002297.2018.1495976 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kressirer, Christine A Chen, Tsute Lake Harriman, Kristie Frias-Lopez, Jorge Dewhirst, Floyd E Tavares, Mary A Tanner, Anne CR Functional profiles of coronal and dentin caries in children |
title | Functional profiles of coronal and dentin caries in children |
title_full | Functional profiles of coronal and dentin caries in children |
title_fullStr | Functional profiles of coronal and dentin caries in children |
title_full_unstemmed | Functional profiles of coronal and dentin caries in children |
title_short | Functional profiles of coronal and dentin caries in children |
title_sort | functional profiles of coronal and dentin caries in children |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052428/ https://www.ncbi.nlm.nih.gov/pubmed/30034639 http://dx.doi.org/10.1080/20002297.2018.1495976 |
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