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Metformin directly targets the H3K27me3 demethylase KDM6A/UTX
Metformin, the first drug chosen to be tested in a clinical trial aimed to target the biology of aging per se, has been clinically exploited for decades in the absence of a complete understanding of its therapeutic targets or chemical determinants. We here outline a systematic chemoinformatics appro...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052472/ https://www.ncbi.nlm.nih.gov/pubmed/29740925 http://dx.doi.org/10.1111/acel.12772 |
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author | Cuyàs, Elisabet Verdura, Sara Llorach‐Pares, Laura Fernández‐Arroyo, Salvador Luciano‐Mateo, Fedra Cabré, Noemí Stursa, Jan Werner, Lukas Martin‐Castillo, Begoña Viollet, Benoit Neuzil, Jiri Joven, Jorge Nonell‐Canals, Alfons Sanchez‐Martinez, Melchor Menendez, Javier A. |
author_facet | Cuyàs, Elisabet Verdura, Sara Llorach‐Pares, Laura Fernández‐Arroyo, Salvador Luciano‐Mateo, Fedra Cabré, Noemí Stursa, Jan Werner, Lukas Martin‐Castillo, Begoña Viollet, Benoit Neuzil, Jiri Joven, Jorge Nonell‐Canals, Alfons Sanchez‐Martinez, Melchor Menendez, Javier A. |
author_sort | Cuyàs, Elisabet |
collection | PubMed |
description | Metformin, the first drug chosen to be tested in a clinical trial aimed to target the biology of aging per se, has been clinically exploited for decades in the absence of a complete understanding of its therapeutic targets or chemical determinants. We here outline a systematic chemoinformatics approach to computationally predict biomolecular targets of metformin. Using several structure‐ and ligand‐based software tools and reference databases containing 1,300,000 chemical compounds and more than 9,000 binding sites protein cavities, we identified 41 putative metformin targets including several epigenetic modifiers such as the member of the H3K27me3‐specific demethylase subfamily, KDM6A/UTX. AlphaScreen and AlphaLISA assays confirmed the ability of metformin to inhibit the demethylation activity of purified KDM6A/UTX enzyme. Structural studies revealed that metformin might occupy the same set of residues involved in H3K27me3 binding and demethylation within the catalytic pocket of KDM6A/UTX. Millimolar metformin augmented global levels of H3K27me3 in cultured cells, including reversion of global loss of H3K27me3 occurring in premature aging syndromes, irrespective of mitochondrial complex I or AMPK. Pharmacological doses of metformin in drinking water or intraperitoneal injection significantly elevated the global levels of H3K27me3 in the hepatic tissue of low‐density lipoprotein receptor‐deficient mice and in the tumor tissues of highly aggressive breast cancer xenograft‐bearing mice. Moreover, nondiabetic breast cancer patients receiving oral metformin in addition to standard therapy presented an elevated level of circulating H3K27me3. Our biocomputational approach coupled to experimental validation reveals that metformin might directly regulate the biological machinery of aging by targeting core chromatin modifiers of the epigenome. |
format | Online Article Text |
id | pubmed-6052472 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60524722018-08-01 Metformin directly targets the H3K27me3 demethylase KDM6A/UTX Cuyàs, Elisabet Verdura, Sara Llorach‐Pares, Laura Fernández‐Arroyo, Salvador Luciano‐Mateo, Fedra Cabré, Noemí Stursa, Jan Werner, Lukas Martin‐Castillo, Begoña Viollet, Benoit Neuzil, Jiri Joven, Jorge Nonell‐Canals, Alfons Sanchez‐Martinez, Melchor Menendez, Javier A. Aging Cell Original Articles Metformin, the first drug chosen to be tested in a clinical trial aimed to target the biology of aging per se, has been clinically exploited for decades in the absence of a complete understanding of its therapeutic targets or chemical determinants. We here outline a systematic chemoinformatics approach to computationally predict biomolecular targets of metformin. Using several structure‐ and ligand‐based software tools and reference databases containing 1,300,000 chemical compounds and more than 9,000 binding sites protein cavities, we identified 41 putative metformin targets including several epigenetic modifiers such as the member of the H3K27me3‐specific demethylase subfamily, KDM6A/UTX. AlphaScreen and AlphaLISA assays confirmed the ability of metformin to inhibit the demethylation activity of purified KDM6A/UTX enzyme. Structural studies revealed that metformin might occupy the same set of residues involved in H3K27me3 binding and demethylation within the catalytic pocket of KDM6A/UTX. Millimolar metformin augmented global levels of H3K27me3 in cultured cells, including reversion of global loss of H3K27me3 occurring in premature aging syndromes, irrespective of mitochondrial complex I or AMPK. Pharmacological doses of metformin in drinking water or intraperitoneal injection significantly elevated the global levels of H3K27me3 in the hepatic tissue of low‐density lipoprotein receptor‐deficient mice and in the tumor tissues of highly aggressive breast cancer xenograft‐bearing mice. Moreover, nondiabetic breast cancer patients receiving oral metformin in addition to standard therapy presented an elevated level of circulating H3K27me3. Our biocomputational approach coupled to experimental validation reveals that metformin might directly regulate the biological machinery of aging by targeting core chromatin modifiers of the epigenome. John Wiley and Sons Inc. 2018-05-08 2018-08 /pmc/articles/PMC6052472/ /pubmed/29740925 http://dx.doi.org/10.1111/acel.12772 Text en © 2018 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Cuyàs, Elisabet Verdura, Sara Llorach‐Pares, Laura Fernández‐Arroyo, Salvador Luciano‐Mateo, Fedra Cabré, Noemí Stursa, Jan Werner, Lukas Martin‐Castillo, Begoña Viollet, Benoit Neuzil, Jiri Joven, Jorge Nonell‐Canals, Alfons Sanchez‐Martinez, Melchor Menendez, Javier A. Metformin directly targets the H3K27me3 demethylase KDM6A/UTX |
title | Metformin directly targets the H3K27me3 demethylase KDM6A/UTX |
title_full | Metformin directly targets the H3K27me3 demethylase KDM6A/UTX |
title_fullStr | Metformin directly targets the H3K27me3 demethylase KDM6A/UTX |
title_full_unstemmed | Metformin directly targets the H3K27me3 demethylase KDM6A/UTX |
title_short | Metformin directly targets the H3K27me3 demethylase KDM6A/UTX |
title_sort | metformin directly targets the h3k27me3 demethylase kdm6a/utx |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052472/ https://www.ncbi.nlm.nih.gov/pubmed/29740925 http://dx.doi.org/10.1111/acel.12772 |
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