Ciliostasis of airway epithelial cells facilitates influenza A virus infection

Porcine precision-cut lung slices (PCLS) were used to analyze the effect of the ciliary activity on infection of airway epithelial cells by influenza viruses. Treatment of slices with 2% NaCl for 30 min resulted in reversible ciliostasis. When PCLS were infected by a swine influenza virus of the H3N...

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Autores principales: Fu, Yuguang, Tong, Jie, Meng, Fandan, Hoeltig, Doris, Liu, Guangliang, Yin, Xiangping, Herrler, Georg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052543/
https://www.ncbi.nlm.nih.gov/pubmed/30021653
http://dx.doi.org/10.1186/s13567-018-0568-0
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author Fu, Yuguang
Tong, Jie
Meng, Fandan
Hoeltig, Doris
Liu, Guangliang
Yin, Xiangping
Herrler, Georg
author_facet Fu, Yuguang
Tong, Jie
Meng, Fandan
Hoeltig, Doris
Liu, Guangliang
Yin, Xiangping
Herrler, Georg
author_sort Fu, Yuguang
collection PubMed
description Porcine precision-cut lung slices (PCLS) were used to analyze the effect of the ciliary activity on infection of airway epithelial cells by influenza viruses. Treatment of slices with 2% NaCl for 30 min resulted in reversible ciliostasis. When PCLS were infected by a swine influenza virus of the H3N2 subtype under ciliostatic conditions, the viral yield was about twofold or threefold higher at 24 or 48 h post-infection, respectively, as compared to slices with ciliary activity. Therefore, the cilia beating not only transports the mucus out of the airways, it also impedes virus infection.
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spelling pubmed-60525432018-07-20 Ciliostasis of airway epithelial cells facilitates influenza A virus infection Fu, Yuguang Tong, Jie Meng, Fandan Hoeltig, Doris Liu, Guangliang Yin, Xiangping Herrler, Georg Vet Res Short Report Porcine precision-cut lung slices (PCLS) were used to analyze the effect of the ciliary activity on infection of airway epithelial cells by influenza viruses. Treatment of slices with 2% NaCl for 30 min resulted in reversible ciliostasis. When PCLS were infected by a swine influenza virus of the H3N2 subtype under ciliostatic conditions, the viral yield was about twofold or threefold higher at 24 or 48 h post-infection, respectively, as compared to slices with ciliary activity. Therefore, the cilia beating not only transports the mucus out of the airways, it also impedes virus infection. BioMed Central 2018-07-18 2018 /pmc/articles/PMC6052543/ /pubmed/30021653 http://dx.doi.org/10.1186/s13567-018-0568-0 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Fu, Yuguang
Tong, Jie
Meng, Fandan
Hoeltig, Doris
Liu, Guangliang
Yin, Xiangping
Herrler, Georg
Ciliostasis of airway epithelial cells facilitates influenza A virus infection
title Ciliostasis of airway epithelial cells facilitates influenza A virus infection
title_full Ciliostasis of airway epithelial cells facilitates influenza A virus infection
title_fullStr Ciliostasis of airway epithelial cells facilitates influenza A virus infection
title_full_unstemmed Ciliostasis of airway epithelial cells facilitates influenza A virus infection
title_short Ciliostasis of airway epithelial cells facilitates influenza A virus infection
title_sort ciliostasis of airway epithelial cells facilitates influenza a virus infection
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052543/
https://www.ncbi.nlm.nih.gov/pubmed/30021653
http://dx.doi.org/10.1186/s13567-018-0568-0
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