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Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent

BACKGROUND: Propolis is a natural substance produced by bees and is known to have antimicrobial activity. Our aim was to evaluate the antimicrobial effect of micellar nanocomposites loaded with an ethyl acetate extract of Brazilian red propolis as a cavity cleaning agent and its influence on the col...

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Autores principales: Celerino de Moraes Porto, Isabel Cristina, Chaves Cardoso de Almeida, Dayse, Vasconcelos Calheiros de Oliveira Costa, Gabriela, Sampaio Donato, Tayná Stéphanie, Moreira Nunes, Letícia, Gomes do Nascimento, Ticiano, dos Santos Oliveira, José Marcos, Batista da Silva, Carolina, Barbosa dos Santos, Natanael, de Alencar e Silva Leite, Maria Luísa, Diniz Basílio-Júnior, Irinaldo, Braga Dornelas, Camila, Barnabé Escodro, Pierre, da Silva Fonseca, Eduardo Jorge, Umeko Kamiya, Regianne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052596/
https://www.ncbi.nlm.nih.gov/pubmed/30021632
http://dx.doi.org/10.1186/s12906-018-2281-y
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author Celerino de Moraes Porto, Isabel Cristina
Chaves Cardoso de Almeida, Dayse
Vasconcelos Calheiros de Oliveira Costa, Gabriela
Sampaio Donato, Tayná Stéphanie
Moreira Nunes, Letícia
Gomes do Nascimento, Ticiano
dos Santos Oliveira, José Marcos
Batista da Silva, Carolina
Barbosa dos Santos, Natanael
de Alencar e Silva Leite, Maria Luísa
Diniz Basílio-Júnior, Irinaldo
Braga Dornelas, Camila
Barnabé Escodro, Pierre
da Silva Fonseca, Eduardo Jorge
Umeko Kamiya, Regianne
author_facet Celerino de Moraes Porto, Isabel Cristina
Chaves Cardoso de Almeida, Dayse
Vasconcelos Calheiros de Oliveira Costa, Gabriela
Sampaio Donato, Tayná Stéphanie
Moreira Nunes, Letícia
Gomes do Nascimento, Ticiano
dos Santos Oliveira, José Marcos
Batista da Silva, Carolina
Barbosa dos Santos, Natanael
de Alencar e Silva Leite, Maria Luísa
Diniz Basílio-Júnior, Irinaldo
Braga Dornelas, Camila
Barnabé Escodro, Pierre
da Silva Fonseca, Eduardo Jorge
Umeko Kamiya, Regianne
author_sort Celerino de Moraes Porto, Isabel Cristina
collection PubMed
description BACKGROUND: Propolis is a natural substance produced by bees and is known to have antimicrobial activity. Our aim was to evaluate the antimicrobial effect of micellar nanocomposites loaded with an ethyl acetate extract of Brazilian red propolis as a cavity cleaning agent and its influence on the color and microtensile bond strength (μTBS) of the dentin/resin interface. METHODS: An ultra-performance liquid chromatography coupled with a diode array detector (UPLC-DAD) assay was used to determine the flavonoids and isoflavones present in an ethyl acetate extract of Brazilian red propolis (EARP) and micellar nanocomposites loaded with EARP (MNRP). The antimicrobial activity of EARP and MNRP was tested against Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. One of the following experimental treatments was applied to etched dentin (phosphoric acid, 15 s): 5 μL of MNRP (RP3, 0.3%; RP6, 0.6%; or RP1, 1.0% w/v), placebo, and 2% chlorhexidine digluconate. Single Bond adhesive (3 M/ESPE) was applied and a 4-mm-thick resin crown (Z350XT, 3 M/ESPE) was built up. After 24 h, the teeth were sectioned into sticks for the μTBS test and scanning electron microscopy. Spectrophotometry according to the CIE L*a*b* chromatic space was used to evaluate the color. Data were analyzed using one-way ANOVA and the Tukey test or Kruskal-Wallis test and the same test for pairwise comparisons between the means (P < 0.05). RESULTS: The UPLC-DAD assay identified the flavonoids liquiritigenin, pinobanksin, pinocembrin, and isoliquiritigenin and the isoflavonoids daidzein, formononetin, and biochanin A in the EARP and micellar nanocomposites. EARP and MNRP presented antimicrobial activity against the cariogenic bacteria Streptococcus mutans and Lactobacillus acidophilus, and for Candida albicans. ΔE values varied from 2.31 to 3.67 (P = 0.457). The mean μTBS for RP1 was significantly lower than for the other groups (P < 0.001). Dentin treated with RP1 showed the shortest resin tags followed by RP6 and RP3. CONCLUSIONS: The EARP and (MNRP) showed antimicrobial activity for the main agents causing dental caries (Streptococcus mutans and Lactobacillus acidophilus) and for Candida albicans. MNRP at concentrations of 0.3 and 0.6% used as a cavity cleaner do not compromise the aesthetics or μTBS of the dentin/resin interface.
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spelling pubmed-60525962018-07-20 Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent Celerino de Moraes Porto, Isabel Cristina Chaves Cardoso de Almeida, Dayse Vasconcelos Calheiros de Oliveira Costa, Gabriela Sampaio Donato, Tayná Stéphanie Moreira Nunes, Letícia Gomes do Nascimento, Ticiano dos Santos Oliveira, José Marcos Batista da Silva, Carolina Barbosa dos Santos, Natanael de Alencar e Silva Leite, Maria Luísa Diniz Basílio-Júnior, Irinaldo Braga Dornelas, Camila Barnabé Escodro, Pierre da Silva Fonseca, Eduardo Jorge Umeko Kamiya, Regianne BMC Complement Altern Med Research Article BACKGROUND: Propolis is a natural substance produced by bees and is known to have antimicrobial activity. Our aim was to evaluate the antimicrobial effect of micellar nanocomposites loaded with an ethyl acetate extract of Brazilian red propolis as a cavity cleaning agent and its influence on the color and microtensile bond strength (μTBS) of the dentin/resin interface. METHODS: An ultra-performance liquid chromatography coupled with a diode array detector (UPLC-DAD) assay was used to determine the flavonoids and isoflavones present in an ethyl acetate extract of Brazilian red propolis (EARP) and micellar nanocomposites loaded with EARP (MNRP). The antimicrobial activity of EARP and MNRP was tested against Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. One of the following experimental treatments was applied to etched dentin (phosphoric acid, 15 s): 5 μL of MNRP (RP3, 0.3%; RP6, 0.6%; or RP1, 1.0% w/v), placebo, and 2% chlorhexidine digluconate. Single Bond adhesive (3 M/ESPE) was applied and a 4-mm-thick resin crown (Z350XT, 3 M/ESPE) was built up. After 24 h, the teeth were sectioned into sticks for the μTBS test and scanning electron microscopy. Spectrophotometry according to the CIE L*a*b* chromatic space was used to evaluate the color. Data were analyzed using one-way ANOVA and the Tukey test or Kruskal-Wallis test and the same test for pairwise comparisons between the means (P < 0.05). RESULTS: The UPLC-DAD assay identified the flavonoids liquiritigenin, pinobanksin, pinocembrin, and isoliquiritigenin and the isoflavonoids daidzein, formononetin, and biochanin A in the EARP and micellar nanocomposites. EARP and MNRP presented antimicrobial activity against the cariogenic bacteria Streptococcus mutans and Lactobacillus acidophilus, and for Candida albicans. ΔE values varied from 2.31 to 3.67 (P = 0.457). The mean μTBS for RP1 was significantly lower than for the other groups (P < 0.001). Dentin treated with RP1 showed the shortest resin tags followed by RP6 and RP3. CONCLUSIONS: The EARP and (MNRP) showed antimicrobial activity for the main agents causing dental caries (Streptococcus mutans and Lactobacillus acidophilus) and for Candida albicans. MNRP at concentrations of 0.3 and 0.6% used as a cavity cleaner do not compromise the aesthetics or μTBS of the dentin/resin interface. BioMed Central 2018-07-18 /pmc/articles/PMC6052596/ /pubmed/30021632 http://dx.doi.org/10.1186/s12906-018-2281-y Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Celerino de Moraes Porto, Isabel Cristina
Chaves Cardoso de Almeida, Dayse
Vasconcelos Calheiros de Oliveira Costa, Gabriela
Sampaio Donato, Tayná Stéphanie
Moreira Nunes, Letícia
Gomes do Nascimento, Ticiano
dos Santos Oliveira, José Marcos
Batista da Silva, Carolina
Barbosa dos Santos, Natanael
de Alencar e Silva Leite, Maria Luísa
Diniz Basílio-Júnior, Irinaldo
Braga Dornelas, Camila
Barnabé Escodro, Pierre
da Silva Fonseca, Eduardo Jorge
Umeko Kamiya, Regianne
Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent
title Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent
title_full Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent
title_fullStr Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent
title_full_unstemmed Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent
title_short Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent
title_sort mechanical and aesthetics compatibility of brazilian red propolis micellar nanocomposite as a cavity cleaning agent
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052596/
https://www.ncbi.nlm.nih.gov/pubmed/30021632
http://dx.doi.org/10.1186/s12906-018-2281-y
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