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Occurrence and characterization of hyperviscous K1 and K2 serotype in Klebsiella pneumoniae

BACKGROUND: Klebsiella pneumoniae causes both nosocomial and community-associated infections. Hypervirulent K. pneumoniae (hvKP), new variant of K. pneumoniae, can cause invasive infections in young healthy individuals as well as in the immunocompromised population. Hypervirulent strains frequently...

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Autores principales: Remya, Poothakuzhiyil, Shanthi, Mariappan, Sekar, Uma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052812/
https://www.ncbi.nlm.nih.gov/pubmed/30078963
http://dx.doi.org/10.4103/JLP.JLP_48_18
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author Remya, Poothakuzhiyil
Shanthi, Mariappan
Sekar, Uma
author_facet Remya, Poothakuzhiyil
Shanthi, Mariappan
Sekar, Uma
author_sort Remya, Poothakuzhiyil
collection PubMed
description BACKGROUND: Klebsiella pneumoniae causes both nosocomial and community-associated infections. Hypervirulent K. pneumoniae (hvKP), new variant of K. pneumoniae, can cause invasive infections in young healthy individuals as well as in the immunocompromised population. Hypervirulent strains frequently belong to capsular serotypes K1 or K2. Emergence of antimicrobial resistance in hvKP is a cause for concern. AIM AND OBJECTIVE: The present study was done to detect the K1 and K2 serotypes among clinical isolates of K. pneumoniae, spectrum of infections caused by them and presence of common beta-lactamases encoding genes in them. MATERIALS AND METHODS: A total of 370 isolates of K. pneumoniae, isolated from various clinical samples over a period of 1 year was included in this study. Antibiotic susceptibility testing to various classes of antimicrobials was done as per Clinical and Laboratory Standard Institute guidelines. The presence of K2A (specific to serotype K2), magA (specific to serotype K1), and rmpA genes was detected by multiplex polymerase chain reaction (PCR). Extended-spectrum beta-lactamases (TEM, SHV, and CTX-M), plasmid-mediated AmpCs (MOX, CIT, DHA, ACC, EBC, and FOX), and carbapenemase genes (IMP, VIM, NDM, KPC, and OXA-48) were also determined by PCR. RESULTS: Among the 370 isolates, 8 harbored K2A gene and one harbored magA. rmpA gene was detected in three isolates along with K1 or K2 serotypes. Seven K2A-positive isolates were resistant to one or more classes of antimicrobials. The studied ESBL genes were present in four isolates. Two isolates harbored carbapenemase genes (NDM-1, OXA-48) along with ESBLs. CONCLUSION: K2 serotype is more prevalent among hvKP isolates. They can harbor ESBLs and Carbapenemase genes. K1 serotype is rather uncommon in K. pneumoniae. Acquisition of multidrug-resistant genes by these strains adds to their virulence and limits the treatment options.
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spelling pubmed-60528122018-08-03 Occurrence and characterization of hyperviscous K1 and K2 serotype in Klebsiella pneumoniae Remya, Poothakuzhiyil Shanthi, Mariappan Sekar, Uma J Lab Physicians Original Article BACKGROUND: Klebsiella pneumoniae causes both nosocomial and community-associated infections. Hypervirulent K. pneumoniae (hvKP), new variant of K. pneumoniae, can cause invasive infections in young healthy individuals as well as in the immunocompromised population. Hypervirulent strains frequently belong to capsular serotypes K1 or K2. Emergence of antimicrobial resistance in hvKP is a cause for concern. AIM AND OBJECTIVE: The present study was done to detect the K1 and K2 serotypes among clinical isolates of K. pneumoniae, spectrum of infections caused by them and presence of common beta-lactamases encoding genes in them. MATERIALS AND METHODS: A total of 370 isolates of K. pneumoniae, isolated from various clinical samples over a period of 1 year was included in this study. Antibiotic susceptibility testing to various classes of antimicrobials was done as per Clinical and Laboratory Standard Institute guidelines. The presence of K2A (specific to serotype K2), magA (specific to serotype K1), and rmpA genes was detected by multiplex polymerase chain reaction (PCR). Extended-spectrum beta-lactamases (TEM, SHV, and CTX-M), plasmid-mediated AmpCs (MOX, CIT, DHA, ACC, EBC, and FOX), and carbapenemase genes (IMP, VIM, NDM, KPC, and OXA-48) were also determined by PCR. RESULTS: Among the 370 isolates, 8 harbored K2A gene and one harbored magA. rmpA gene was detected in three isolates along with K1 or K2 serotypes. Seven K2A-positive isolates were resistant to one or more classes of antimicrobials. The studied ESBL genes were present in four isolates. Two isolates harbored carbapenemase genes (NDM-1, OXA-48) along with ESBLs. CONCLUSION: K2 serotype is more prevalent among hvKP isolates. They can harbor ESBLs and Carbapenemase genes. K1 serotype is rather uncommon in K. pneumoniae. Acquisition of multidrug-resistant genes by these strains adds to their virulence and limits the treatment options. Medknow Publications & Media Pvt Ltd 2018 /pmc/articles/PMC6052812/ /pubmed/30078963 http://dx.doi.org/10.4103/JLP.JLP_48_18 Text en Copyright: © 2018 Journal of Laboratory Physicians http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Remya, Poothakuzhiyil
Shanthi, Mariappan
Sekar, Uma
Occurrence and characterization of hyperviscous K1 and K2 serotype in Klebsiella pneumoniae
title Occurrence and characterization of hyperviscous K1 and K2 serotype in Klebsiella pneumoniae
title_full Occurrence and characterization of hyperviscous K1 and K2 serotype in Klebsiella pneumoniae
title_fullStr Occurrence and characterization of hyperviscous K1 and K2 serotype in Klebsiella pneumoniae
title_full_unstemmed Occurrence and characterization of hyperviscous K1 and K2 serotype in Klebsiella pneumoniae
title_short Occurrence and characterization of hyperviscous K1 and K2 serotype in Klebsiella pneumoniae
title_sort occurrence and characterization of hyperviscous k1 and k2 serotype in klebsiella pneumoniae
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052812/
https://www.ncbi.nlm.nih.gov/pubmed/30078963
http://dx.doi.org/10.4103/JLP.JLP_48_18
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