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Diagnostic algorithm for determining primary tumor sites using peritoneal fluid

This study was conducted to develop a novel algorithm for determining the origin of tumors by combining analysis of cluster patterns with immunocytochemistry (ICC) for markers in cells from fine-needle aspirates of ascites. We used LBC, based on SurePath(TM) (BD Diagnostics) technology, to screen 96...

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Detalles Bibliográficos
Autores principales: Park, Cheol Keun, Malinowski, Douglas P., Cho, Nam Hoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6053134/
https://www.ncbi.nlm.nih.gov/pubmed/30024911
http://dx.doi.org/10.1371/journal.pone.0199715
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author Park, Cheol Keun
Malinowski, Douglas P.
Cho, Nam Hoon
author_facet Park, Cheol Keun
Malinowski, Douglas P.
Cho, Nam Hoon
author_sort Park, Cheol Keun
collection PubMed
description This study was conducted to develop a novel algorithm for determining the origin of tumors by combining analysis of cluster patterns with immunocytochemistry (ICC) for markers in cells from fine-needle aspirates of ascites. We used LBC, based on SurePath(TM) (BD Diagnostics) technology, to screen 96 peritoneal fluid samples from patients with known malignancies and from 10 control patients with cirrhosis. Following dual ICC staining for cytokeratin 7 (CK7) and paired box gene 8 (PAX8), we developed an algorithm using immunoreactivity and three-dimensional (3D) cluster patterns to correlate staining and 3D cluster patterns with common primary origins that included stomach, ovarian, pancreatobiliary tract, colon, lung, and breast cancers. With the application of an automatic digitalized image analyzer, competence performance was analyzed using receiver operating characteristics (ROC) curve analysis. CK7 and PAX8 staining and 3D cluster patterns were used to differentiate primary origins. Samples from patients with stomach cancer were no 3D cluster /CK7(+)/PAX8(-) with area under the curve (AUC) of 0.8699 in ROC curve analysis. Samples from ovarian cancer patients were large 3D cluster/CK7(+)/PAX8(+) with AUC of 0.9812. Samples from pancreatobiliary tract cancer patients were small 3D cluster/CK7(+)/PAX8(-) with AUC of 0.8772. The remaining cancer samples, including breast, lung and colon cancer samples, had similar patterns of large 3D clusters/CK7(+)/PAX8(-) with AUC of 0.882, especially for lung cancer. SurePath(TM) technology, using 3D cluster patterns and dual ICC for CK7 and PAX8 in peritoneal fluid samples, can provide important information for determining specific primary origins in cases of unknown primary carcinoma.
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spelling pubmed-60531342018-07-27 Diagnostic algorithm for determining primary tumor sites using peritoneal fluid Park, Cheol Keun Malinowski, Douglas P. Cho, Nam Hoon PLoS One Research Article This study was conducted to develop a novel algorithm for determining the origin of tumors by combining analysis of cluster patterns with immunocytochemistry (ICC) for markers in cells from fine-needle aspirates of ascites. We used LBC, based on SurePath(TM) (BD Diagnostics) technology, to screen 96 peritoneal fluid samples from patients with known malignancies and from 10 control patients with cirrhosis. Following dual ICC staining for cytokeratin 7 (CK7) and paired box gene 8 (PAX8), we developed an algorithm using immunoreactivity and three-dimensional (3D) cluster patterns to correlate staining and 3D cluster patterns with common primary origins that included stomach, ovarian, pancreatobiliary tract, colon, lung, and breast cancers. With the application of an automatic digitalized image analyzer, competence performance was analyzed using receiver operating characteristics (ROC) curve analysis. CK7 and PAX8 staining and 3D cluster patterns were used to differentiate primary origins. Samples from patients with stomach cancer were no 3D cluster /CK7(+)/PAX8(-) with area under the curve (AUC) of 0.8699 in ROC curve analysis. Samples from ovarian cancer patients were large 3D cluster/CK7(+)/PAX8(+) with AUC of 0.9812. Samples from pancreatobiliary tract cancer patients were small 3D cluster/CK7(+)/PAX8(-) with AUC of 0.8772. The remaining cancer samples, including breast, lung and colon cancer samples, had similar patterns of large 3D clusters/CK7(+)/PAX8(-) with AUC of 0.882, especially for lung cancer. SurePath(TM) technology, using 3D cluster patterns and dual ICC for CK7 and PAX8 in peritoneal fluid samples, can provide important information for determining specific primary origins in cases of unknown primary carcinoma. Public Library of Science 2018-07-19 /pmc/articles/PMC6053134/ /pubmed/30024911 http://dx.doi.org/10.1371/journal.pone.0199715 Text en © 2018 Park et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Park, Cheol Keun
Malinowski, Douglas P.
Cho, Nam Hoon
Diagnostic algorithm for determining primary tumor sites using peritoneal fluid
title Diagnostic algorithm for determining primary tumor sites using peritoneal fluid
title_full Diagnostic algorithm for determining primary tumor sites using peritoneal fluid
title_fullStr Diagnostic algorithm for determining primary tumor sites using peritoneal fluid
title_full_unstemmed Diagnostic algorithm for determining primary tumor sites using peritoneal fluid
title_short Diagnostic algorithm for determining primary tumor sites using peritoneal fluid
title_sort diagnostic algorithm for determining primary tumor sites using peritoneal fluid
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6053134/
https://www.ncbi.nlm.nih.gov/pubmed/30024911
http://dx.doi.org/10.1371/journal.pone.0199715
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