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Genomic organisation and alternative splicing of mouse and human thioredoxin reductase 1 genes

BACKGROUND: Thioredoxin reductase (TR) is a redox active protein involved in many cellular processes as part of the thioredoxin system. Presently there are three recognised forms of mammalian thioredoxin reductase designated as TR1, TR3 and TGR, that represent the cytosolic, mitochondrial and novel...

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Autores principales: Osborne, Simone A, Tonissen, Kathryn F
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC60534/
https://www.ncbi.nlm.nih.gov/pubmed/11737861
http://dx.doi.org/10.1186/1471-2164-2-10
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author Osborne, Simone A
Tonissen, Kathryn F
author_facet Osborne, Simone A
Tonissen, Kathryn F
author_sort Osborne, Simone A
collection PubMed
description BACKGROUND: Thioredoxin reductase (TR) is a redox active protein involved in many cellular processes as part of the thioredoxin system. Presently there are three recognised forms of mammalian thioredoxin reductase designated as TR1, TR3 and TGR, that represent the cytosolic, mitochondrial and novel forms respectively. In this study we elucidated the genomic organisation of the mouse (Txnrd1) and human thioredoxin reductase 1 genes (TXNRD1) through library screening, restriction mapping and database mining. RESULTS: The human TXNRD1 gene spans 100 kb of genomic DNA organised into 16 exons and the mouse Txnrd1 gene has a similar exon/intron arrangement. We also analysed the alternative splicing patterns displayed by the mouse and human thioredoxin reductase 1 genes and mapped the different mRNA isoforms with respect to genomic organisation. These isoforms differ at the 5' end and encode putative proteins of different molecular mass. Genomic DNA sequences upstream of mouse exon 1 were compared to the human promoter to identify conserved elements. CONCLUSIONS: The human and mouse thioredoxin reductase 1 gene organisation is highly conserved and both genes exhibit alternative splicing at the 5' end. The mouse and human promoters share some conserved sequences.
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spelling pubmed-605342001-12-07 Genomic organisation and alternative splicing of mouse and human thioredoxin reductase 1 genes Osborne, Simone A Tonissen, Kathryn F BMC Genomics Research Article BACKGROUND: Thioredoxin reductase (TR) is a redox active protein involved in many cellular processes as part of the thioredoxin system. Presently there are three recognised forms of mammalian thioredoxin reductase designated as TR1, TR3 and TGR, that represent the cytosolic, mitochondrial and novel forms respectively. In this study we elucidated the genomic organisation of the mouse (Txnrd1) and human thioredoxin reductase 1 genes (TXNRD1) through library screening, restriction mapping and database mining. RESULTS: The human TXNRD1 gene spans 100 kb of genomic DNA organised into 16 exons and the mouse Txnrd1 gene has a similar exon/intron arrangement. We also analysed the alternative splicing patterns displayed by the mouse and human thioredoxin reductase 1 genes and mapped the different mRNA isoforms with respect to genomic organisation. These isoforms differ at the 5' end and encode putative proteins of different molecular mass. Genomic DNA sequences upstream of mouse exon 1 were compared to the human promoter to identify conserved elements. CONCLUSIONS: The human and mouse thioredoxin reductase 1 gene organisation is highly conserved and both genes exhibit alternative splicing at the 5' end. The mouse and human promoters share some conserved sequences. BioMed Central 2001-11-22 /pmc/articles/PMC60534/ /pubmed/11737861 http://dx.doi.org/10.1186/1471-2164-2-10 Text en Copyright © 2001 Osborne and Tonissen; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Osborne, Simone A
Tonissen, Kathryn F
Genomic organisation and alternative splicing of mouse and human thioredoxin reductase 1 genes
title Genomic organisation and alternative splicing of mouse and human thioredoxin reductase 1 genes
title_full Genomic organisation and alternative splicing of mouse and human thioredoxin reductase 1 genes
title_fullStr Genomic organisation and alternative splicing of mouse and human thioredoxin reductase 1 genes
title_full_unstemmed Genomic organisation and alternative splicing of mouse and human thioredoxin reductase 1 genes
title_short Genomic organisation and alternative splicing of mouse and human thioredoxin reductase 1 genes
title_sort genomic organisation and alternative splicing of mouse and human thioredoxin reductase 1 genes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC60534/
https://www.ncbi.nlm.nih.gov/pubmed/11737861
http://dx.doi.org/10.1186/1471-2164-2-10
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