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Identification and characterization of known and novel microRNAs in strawberry fruits induced by Botrytis cinerea

MicroRNAs are endogenous small non-coding RNAs that negatively regulate mRNAs, mainly at the post-transcriptional level, and play an important role in resistance response of plants. To date, there are few reports on resistance response of strawberry miRNAs to pathogens. In this study, using high-thr...

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Autores principales: Liang, Yaoxin, Guan, Yuhan, Wang, Shaoxi, Li, Yanjun, Zhang, Zhihong, Li, He
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6053406/
https://www.ncbi.nlm.nih.gov/pubmed/30026481
http://dx.doi.org/10.1038/s41598-018-29289-7
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author Liang, Yaoxin
Guan, Yuhan
Wang, Shaoxi
Li, Yanjun
Zhang, Zhihong
Li, He
author_facet Liang, Yaoxin
Guan, Yuhan
Wang, Shaoxi
Li, Yanjun
Zhang, Zhihong
Li, He
author_sort Liang, Yaoxin
collection PubMed
description MicroRNAs are endogenous small non-coding RNAs that negatively regulate mRNAs, mainly at the post-transcriptional level, and play an important role in resistance response of plants. To date, there are few reports on resistance response of strawberry miRNAs to pathogens. In this study, using high-throughput sequencing, 134 conserved and 35 novel miRNAs were identified in six libraries within the treatment of Botrytis cinerea. A total 497 potential target genes were predicted using Fragaria vesca genome. Most of the differential expressed miRNAs in strawberry fruits were up-regulated in early libraries and down-regulated in late libraries. PIRL, the target gene of miR5290a, showed the opposite expressed trend compared with miR5290 from T1 to T3 libraries, and functional analysis of the PIRL gene shows that it has obvious resistance to B. cinerea in the strawberry fruits with overexpressed PIRL gene. We speculate that miR5290a negatively regulates its target gene PIRL to increase resistance to pathogen infection, and further analysis of PIRL function is meaningful for studying the plant-pathogen relationship and improving strawberry fruit quality and yield.
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spelling pubmed-60534062018-07-23 Identification and characterization of known and novel microRNAs in strawberry fruits induced by Botrytis cinerea Liang, Yaoxin Guan, Yuhan Wang, Shaoxi Li, Yanjun Zhang, Zhihong Li, He Sci Rep Article MicroRNAs are endogenous small non-coding RNAs that negatively regulate mRNAs, mainly at the post-transcriptional level, and play an important role in resistance response of plants. To date, there are few reports on resistance response of strawberry miRNAs to pathogens. In this study, using high-throughput sequencing, 134 conserved and 35 novel miRNAs were identified in six libraries within the treatment of Botrytis cinerea. A total 497 potential target genes were predicted using Fragaria vesca genome. Most of the differential expressed miRNAs in strawberry fruits were up-regulated in early libraries and down-regulated in late libraries. PIRL, the target gene of miR5290a, showed the opposite expressed trend compared with miR5290 from T1 to T3 libraries, and functional analysis of the PIRL gene shows that it has obvious resistance to B. cinerea in the strawberry fruits with overexpressed PIRL gene. We speculate that miR5290a negatively regulates its target gene PIRL to increase resistance to pathogen infection, and further analysis of PIRL function is meaningful for studying the plant-pathogen relationship and improving strawberry fruit quality and yield. Nature Publishing Group UK 2018-07-19 /pmc/articles/PMC6053406/ /pubmed/30026481 http://dx.doi.org/10.1038/s41598-018-29289-7 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Liang, Yaoxin
Guan, Yuhan
Wang, Shaoxi
Li, Yanjun
Zhang, Zhihong
Li, He
Identification and characterization of known and novel microRNAs in strawberry fruits induced by Botrytis cinerea
title Identification and characterization of known and novel microRNAs in strawberry fruits induced by Botrytis cinerea
title_full Identification and characterization of known and novel microRNAs in strawberry fruits induced by Botrytis cinerea
title_fullStr Identification and characterization of known and novel microRNAs in strawberry fruits induced by Botrytis cinerea
title_full_unstemmed Identification and characterization of known and novel microRNAs in strawberry fruits induced by Botrytis cinerea
title_short Identification and characterization of known and novel microRNAs in strawberry fruits induced by Botrytis cinerea
title_sort identification and characterization of known and novel micrornas in strawberry fruits induced by botrytis cinerea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6053406/
https://www.ncbi.nlm.nih.gov/pubmed/30026481
http://dx.doi.org/10.1038/s41598-018-29289-7
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