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Age-related changes in vascular responses to angiotensin-(1-7) in female mice
The vasodilatory effect of angiotensin-(1-7) seems to vary between sexes, and estradiol (E2) can modulate the magnitude of the Ang-(1-7) vasodilatory response in female rats. However, there are few studies addressing the influence of sex on the age-related vasodilatory effect of Ang-(1-7). Here, we...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6053867/ https://www.ncbi.nlm.nih.gov/pubmed/30024321 http://dx.doi.org/10.1177/1470320318789332 |
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author | Costa-Fraga, Fabiana P Goncalves, Gleisy K Souza-Neto, Fernando P Reis, Adelina M Capettini, Luciano AS Santos, Robson AS Fraga-Silva, Rodrigo A Stergiopulos, Nikolaos da Silva, Rafaela F |
author_facet | Costa-Fraga, Fabiana P Goncalves, Gleisy K Souza-Neto, Fernando P Reis, Adelina M Capettini, Luciano AS Santos, Robson AS Fraga-Silva, Rodrigo A Stergiopulos, Nikolaos da Silva, Rafaela F |
author_sort | Costa-Fraga, Fabiana P |
collection | PubMed |
description | The vasodilatory effect of angiotensin-(1-7) seems to vary between sexes, and estradiol (E2) can modulate the magnitude of the Ang-(1-7) vasodilatory response in female rats. However, there are few studies addressing the influence of sex on the age-related vasodilatory effect of Ang-(1-7). Here, we evaluated the vasodilatory response to Ang-(1-7) on vascular ageing. Ang-(1-7) dose–response curves were determined in mice aortic rings from males (old and young) and females (E2 treated/non-treated old and young) mounted in an isolated organ chamber. Abdominal aortic rings were used for protein expression analysis and determination of reactive oxygen species (ROS) and nitric oxide (NO) production. Our results showed that the Ang-(1-7) vasodilatory effect was absent in aorta from old females, contrasting with a full response in vessels from young females. The Ang-(1-7) vasodilatory effect was restored by E2 replacement in old females. A robust increase in Mas receptor, SOD2, NRF-2 and NOX2 expression was observed in aorta from old females, which was normalized by E2. This effect of E2 was also associated with lower production of ROS and normal levels of NO. In conclusion, our data demonstrated that pathways involved in the Ang-(1-7) vasodilatory response in female mice is affected by hormonal changes in ageing and rescued by E2. |
format | Online Article Text |
id | pubmed-6053867 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-60538672018-07-23 Age-related changes in vascular responses to angiotensin-(1-7) in female mice Costa-Fraga, Fabiana P Goncalves, Gleisy K Souza-Neto, Fernando P Reis, Adelina M Capettini, Luciano AS Santos, Robson AS Fraga-Silva, Rodrigo A Stergiopulos, Nikolaos da Silva, Rafaela F J Renin Angiotensin Aldosterone Syst Original Article The vasodilatory effect of angiotensin-(1-7) seems to vary between sexes, and estradiol (E2) can modulate the magnitude of the Ang-(1-7) vasodilatory response in female rats. However, there are few studies addressing the influence of sex on the age-related vasodilatory effect of Ang-(1-7). Here, we evaluated the vasodilatory response to Ang-(1-7) on vascular ageing. Ang-(1-7) dose–response curves were determined in mice aortic rings from males (old and young) and females (E2 treated/non-treated old and young) mounted in an isolated organ chamber. Abdominal aortic rings were used for protein expression analysis and determination of reactive oxygen species (ROS) and nitric oxide (NO) production. Our results showed that the Ang-(1-7) vasodilatory effect was absent in aorta from old females, contrasting with a full response in vessels from young females. The Ang-(1-7) vasodilatory effect was restored by E2 replacement in old females. A robust increase in Mas receptor, SOD2, NRF-2 and NOX2 expression was observed in aorta from old females, which was normalized by E2. This effect of E2 was also associated with lower production of ROS and normal levels of NO. In conclusion, our data demonstrated that pathways involved in the Ang-(1-7) vasodilatory response in female mice is affected by hormonal changes in ageing and rescued by E2. SAGE Publications 2018-07-19 /pmc/articles/PMC6053867/ /pubmed/30024321 http://dx.doi.org/10.1177/1470320318789332 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Costa-Fraga, Fabiana P Goncalves, Gleisy K Souza-Neto, Fernando P Reis, Adelina M Capettini, Luciano AS Santos, Robson AS Fraga-Silva, Rodrigo A Stergiopulos, Nikolaos da Silva, Rafaela F Age-related changes in vascular responses to angiotensin-(1-7) in female mice |
title | Age-related changes in vascular responses to angiotensin-(1-7) in
female mice |
title_full | Age-related changes in vascular responses to angiotensin-(1-7) in
female mice |
title_fullStr | Age-related changes in vascular responses to angiotensin-(1-7) in
female mice |
title_full_unstemmed | Age-related changes in vascular responses to angiotensin-(1-7) in
female mice |
title_short | Age-related changes in vascular responses to angiotensin-(1-7) in
female mice |
title_sort | age-related changes in vascular responses to angiotensin-(1-7) in
female mice |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6053867/ https://www.ncbi.nlm.nih.gov/pubmed/30024321 http://dx.doi.org/10.1177/1470320318789332 |
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