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Metagenomic insights into diazotrophic communities across Arctic glacier forefields

Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In to...

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Autores principales: Nash, Maisie V, Anesio, Alexandre M, Barker, Gary, Tranter, Martyn, Varliero, Gilda, Eloe-Fadrosh, Emiley A, Nielsen, Torben, Turpin-Jelfs, Thomas, Benning, Liane G, Sánchez-Baracaldo, Patricia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054269/
https://www.ncbi.nlm.nih.gov/pubmed/29901729
http://dx.doi.org/10.1093/femsec/fiy114
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author Nash, Maisie V
Anesio, Alexandre M
Barker, Gary
Tranter, Martyn
Varliero, Gilda
Eloe-Fadrosh, Emiley A
Nielsen, Torben
Turpin-Jelfs, Thomas
Benning, Liane G
Sánchez-Baracaldo, Patricia
author_facet Nash, Maisie V
Anesio, Alexandre M
Barker, Gary
Tranter, Martyn
Varliero, Gilda
Eloe-Fadrosh, Emiley A
Nielsen, Torben
Turpin-Jelfs, Thomas
Benning, Liane G
Sánchez-Baracaldo, Patricia
author_sort Nash, Maisie V
collection PubMed
description Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes.
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spelling pubmed-60542692018-07-25 Metagenomic insights into diazotrophic communities across Arctic glacier forefields Nash, Maisie V Anesio, Alexandre M Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G Sánchez-Baracaldo, Patricia FEMS Microbiol Ecol Research Article Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes. Oxford University Press 2018-06-12 /pmc/articles/PMC6054269/ /pubmed/29901729 http://dx.doi.org/10.1093/femsec/fiy114 Text en © FEMS 2018. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nash, Maisie V
Anesio, Alexandre M
Barker, Gary
Tranter, Martyn
Varliero, Gilda
Eloe-Fadrosh, Emiley A
Nielsen, Torben
Turpin-Jelfs, Thomas
Benning, Liane G
Sánchez-Baracaldo, Patricia
Metagenomic insights into diazotrophic communities across Arctic glacier forefields
title Metagenomic insights into diazotrophic communities across Arctic glacier forefields
title_full Metagenomic insights into diazotrophic communities across Arctic glacier forefields
title_fullStr Metagenomic insights into diazotrophic communities across Arctic glacier forefields
title_full_unstemmed Metagenomic insights into diazotrophic communities across Arctic glacier forefields
title_short Metagenomic insights into diazotrophic communities across Arctic glacier forefields
title_sort metagenomic insights into diazotrophic communities across arctic glacier forefields
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054269/
https://www.ncbi.nlm.nih.gov/pubmed/29901729
http://dx.doi.org/10.1093/femsec/fiy114
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