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Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids
To detect and study diseases, research and clinical laboratories must quantify specific biomarkers in the plasma and urine of patients with precision, sensitivity, and cost-effectiveness. Newly developed techniques, such as particle-based immunoassays, must be validated in these terms against standa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054379/ https://www.ncbi.nlm.nih.gov/pubmed/30028867 http://dx.doi.org/10.1371/journal.pone.0201009 |
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author | Verma, Sujit Kumar Albrecht, Anja Karin Siebecke, Verena Klöck, Gerd Kolesnikova, Tatiana A. Springer, Sebastian |
author_facet | Verma, Sujit Kumar Albrecht, Anja Karin Siebecke, Verena Klöck, Gerd Kolesnikova, Tatiana A. Springer, Sebastian |
author_sort | Verma, Sujit Kumar |
collection | PubMed |
description | To detect and study diseases, research and clinical laboratories must quantify specific biomarkers in the plasma and urine of patients with precision, sensitivity, and cost-effectiveness. Newly developed techniques, such as particle-based immunoassays, must be validated in these terms against standard methods such as enzyme-linked immunosorbent assays (ELISAs). Here, we compare the performance of assays that use hollow polyelectrolyte microcapsules with assays based on solid plastic beads, and with standard microplate immunoassays. The polyelectrolyte microcapsules detect the disease biomarker beta-2 microglobulin with a fifty-fold increase in sensitivity than polystyrene (PS) beads. For sequence-specific nucleic acid detection, the oligonucleotide-coated microcapsules exhibit a two-fold lower increase in sensitivity over PS beads. The microcapsules also detect the presence of a monoclonal antibody in hybridoma supernatant at a fifty-six-fold increase in sensitivity compared to a microplate assay. Overall, polyelectrolyte microcapsule-based assays are more sensitive for the detection of protein and nucleic acid analytes than PS beads and microplate assays, and they are viable alternatives as a platform for the rapid quantitative detection of analytes at very low concentrations. |
format | Online Article Text |
id | pubmed-6054379 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-60543792018-07-27 Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids Verma, Sujit Kumar Albrecht, Anja Karin Siebecke, Verena Klöck, Gerd Kolesnikova, Tatiana A. Springer, Sebastian PLoS One Research Article To detect and study diseases, research and clinical laboratories must quantify specific biomarkers in the plasma and urine of patients with precision, sensitivity, and cost-effectiveness. Newly developed techniques, such as particle-based immunoassays, must be validated in these terms against standard methods such as enzyme-linked immunosorbent assays (ELISAs). Here, we compare the performance of assays that use hollow polyelectrolyte microcapsules with assays based on solid plastic beads, and with standard microplate immunoassays. The polyelectrolyte microcapsules detect the disease biomarker beta-2 microglobulin with a fifty-fold increase in sensitivity than polystyrene (PS) beads. For sequence-specific nucleic acid detection, the oligonucleotide-coated microcapsules exhibit a two-fold lower increase in sensitivity over PS beads. The microcapsules also detect the presence of a monoclonal antibody in hybridoma supernatant at a fifty-six-fold increase in sensitivity compared to a microplate assay. Overall, polyelectrolyte microcapsule-based assays are more sensitive for the detection of protein and nucleic acid analytes than PS beads and microplate assays, and they are viable alternatives as a platform for the rapid quantitative detection of analytes at very low concentrations. Public Library of Science 2018-07-20 /pmc/articles/PMC6054379/ /pubmed/30028867 http://dx.doi.org/10.1371/journal.pone.0201009 Text en © 2018 Verma et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Verma, Sujit Kumar Albrecht, Anja Karin Siebecke, Verena Klöck, Gerd Kolesnikova, Tatiana A. Springer, Sebastian Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids |
title | Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids |
title_full | Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids |
title_fullStr | Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids |
title_full_unstemmed | Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids |
title_short | Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids |
title_sort | comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054379/ https://www.ncbi.nlm.nih.gov/pubmed/30028867 http://dx.doi.org/10.1371/journal.pone.0201009 |
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