Cargando…

Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids

To detect and study diseases, research and clinical laboratories must quantify specific biomarkers in the plasma and urine of patients with precision, sensitivity, and cost-effectiveness. Newly developed techniques, such as particle-based immunoassays, must be validated in these terms against standa...

Descripción completa

Detalles Bibliográficos
Autores principales: Verma, Sujit Kumar, Albrecht, Anja Karin, Siebecke, Verena, Klöck, Gerd, Kolesnikova, Tatiana A., Springer, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054379/
https://www.ncbi.nlm.nih.gov/pubmed/30028867
http://dx.doi.org/10.1371/journal.pone.0201009
_version_ 1783340993469218816
author Verma, Sujit Kumar
Albrecht, Anja Karin
Siebecke, Verena
Klöck, Gerd
Kolesnikova, Tatiana A.
Springer, Sebastian
author_facet Verma, Sujit Kumar
Albrecht, Anja Karin
Siebecke, Verena
Klöck, Gerd
Kolesnikova, Tatiana A.
Springer, Sebastian
author_sort Verma, Sujit Kumar
collection PubMed
description To detect and study diseases, research and clinical laboratories must quantify specific biomarkers in the plasma and urine of patients with precision, sensitivity, and cost-effectiveness. Newly developed techniques, such as particle-based immunoassays, must be validated in these terms against standard methods such as enzyme-linked immunosorbent assays (ELISAs). Here, we compare the performance of assays that use hollow polyelectrolyte microcapsules with assays based on solid plastic beads, and with standard microplate immunoassays. The polyelectrolyte microcapsules detect the disease biomarker beta-2 microglobulin with a fifty-fold increase in sensitivity than polystyrene (PS) beads. For sequence-specific nucleic acid detection, the oligonucleotide-coated microcapsules exhibit a two-fold lower increase in sensitivity over PS beads. The microcapsules also detect the presence of a monoclonal antibody in hybridoma supernatant at a fifty-six-fold increase in sensitivity compared to a microplate assay. Overall, polyelectrolyte microcapsule-based assays are more sensitive for the detection of protein and nucleic acid analytes than PS beads and microplate assays, and they are viable alternatives as a platform for the rapid quantitative detection of analytes at very low concentrations.
format Online
Article
Text
id pubmed-6054379
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-60543792018-07-27 Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids Verma, Sujit Kumar Albrecht, Anja Karin Siebecke, Verena Klöck, Gerd Kolesnikova, Tatiana A. Springer, Sebastian PLoS One Research Article To detect and study diseases, research and clinical laboratories must quantify specific biomarkers in the plasma and urine of patients with precision, sensitivity, and cost-effectiveness. Newly developed techniques, such as particle-based immunoassays, must be validated in these terms against standard methods such as enzyme-linked immunosorbent assays (ELISAs). Here, we compare the performance of assays that use hollow polyelectrolyte microcapsules with assays based on solid plastic beads, and with standard microplate immunoassays. The polyelectrolyte microcapsules detect the disease biomarker beta-2 microglobulin with a fifty-fold increase in sensitivity than polystyrene (PS) beads. For sequence-specific nucleic acid detection, the oligonucleotide-coated microcapsules exhibit a two-fold lower increase in sensitivity over PS beads. The microcapsules also detect the presence of a monoclonal antibody in hybridoma supernatant at a fifty-six-fold increase in sensitivity compared to a microplate assay. Overall, polyelectrolyte microcapsule-based assays are more sensitive for the detection of protein and nucleic acid analytes than PS beads and microplate assays, and they are viable alternatives as a platform for the rapid quantitative detection of analytes at very low concentrations. Public Library of Science 2018-07-20 /pmc/articles/PMC6054379/ /pubmed/30028867 http://dx.doi.org/10.1371/journal.pone.0201009 Text en © 2018 Verma et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Verma, Sujit Kumar
Albrecht, Anja Karin
Siebecke, Verena
Klöck, Gerd
Kolesnikova, Tatiana A.
Springer, Sebastian
Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids
title Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids
title_full Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids
title_fullStr Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids
title_full_unstemmed Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids
title_short Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids
title_sort comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054379/
https://www.ncbi.nlm.nih.gov/pubmed/30028867
http://dx.doi.org/10.1371/journal.pone.0201009
work_keys_str_mv AT vermasujitkumar comparativevalidationofamicrocapsulebasedimmunoassayforthedetectionofproteinsandnucleicacids
AT albrechtanjakarin comparativevalidationofamicrocapsulebasedimmunoassayforthedetectionofproteinsandnucleicacids
AT siebeckeverena comparativevalidationofamicrocapsulebasedimmunoassayforthedetectionofproteinsandnucleicacids
AT klockgerd comparativevalidationofamicrocapsulebasedimmunoassayforthedetectionofproteinsandnucleicacids
AT kolesnikovatatianaa comparativevalidationofamicrocapsulebasedimmunoassayforthedetectionofproteinsandnucleicacids
AT springersebastian comparativevalidationofamicrocapsulebasedimmunoassayforthedetectionofproteinsandnucleicacids