Intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes

Glycosphingolipids (GSLs) are found in cellular membranes of most organisms and play important roles in cell-cell recognition, signaling, growth, and adhesion, among others. A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC C...

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Autores principales: Wong, Maurice, Xu, Gege, Park, Dayoung, Barboza, Mariana, Lebrilla, Carlito B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054638/
https://www.ncbi.nlm.nih.gov/pubmed/30030471
http://dx.doi.org/10.1038/s41598-018-29324-7
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author Wong, Maurice
Xu, Gege
Park, Dayoung
Barboza, Mariana
Lebrilla, Carlito B.
author_facet Wong, Maurice
Xu, Gege
Park, Dayoung
Barboza, Mariana
Lebrilla, Carlito B.
author_sort Wong, Maurice
collection PubMed
description Glycosphingolipids (GSLs) are found in cellular membranes of most organisms and play important roles in cell-cell recognition, signaling, growth, and adhesion, among others. A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue. The method provides the composition and sequence of the glycan, as well as variations in the ceramide portion of the GSL. It was used to profile the changes in the glycolipidome of Caco-2 cells as they undergo differentiation. A total of 226 unique GSLs were found among Caco-2 samples from five differentiation time-points. The method provided a comprehensive glycolipidomic profile of a cell during differentiation to yield the dynamic variation of intact GSL structures.
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spelling pubmed-60546382018-07-23 Intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes Wong, Maurice Xu, Gege Park, Dayoung Barboza, Mariana Lebrilla, Carlito B. Sci Rep Article Glycosphingolipids (GSLs) are found in cellular membranes of most organisms and play important roles in cell-cell recognition, signaling, growth, and adhesion, among others. A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue. The method provides the composition and sequence of the glycan, as well as variations in the ceramide portion of the GSL. It was used to profile the changes in the glycolipidome of Caco-2 cells as they undergo differentiation. A total of 226 unique GSLs were found among Caco-2 samples from five differentiation time-points. The method provided a comprehensive glycolipidomic profile of a cell during differentiation to yield the dynamic variation of intact GSL structures. Nature Publishing Group UK 2018-07-20 /pmc/articles/PMC6054638/ /pubmed/30030471 http://dx.doi.org/10.1038/s41598-018-29324-7 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wong, Maurice
Xu, Gege
Park, Dayoung
Barboza, Mariana
Lebrilla, Carlito B.
Intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes
title Intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes
title_full Intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes
title_fullStr Intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes
title_full_unstemmed Intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes
title_short Intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes
title_sort intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054638/
https://www.ncbi.nlm.nih.gov/pubmed/30030471
http://dx.doi.org/10.1038/s41598-018-29324-7
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