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Chemically controlled aggregation of pluripotent stem cells

Heterogeneity in pluripotent stem cell (PSC) aggregation leads to variability in mass transfer and signaling gradients between aggregates, which results in heterogeneous differentiation and therefore variability in product quality and yield. We have characterized a chemical‐based method to control a...

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Detalles Bibliográficos
Autores principales: Lipsitz, Yonatan Y., Tonge, Peter D., Zandstra, Peter W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6055717/
https://www.ncbi.nlm.nih.gov/pubmed/29679475
http://dx.doi.org/10.1002/bit.26719
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author Lipsitz, Yonatan Y.
Tonge, Peter D.
Zandstra, Peter W.
author_facet Lipsitz, Yonatan Y.
Tonge, Peter D.
Zandstra, Peter W.
author_sort Lipsitz, Yonatan Y.
collection PubMed
description Heterogeneity in pluripotent stem cell (PSC) aggregation leads to variability in mass transfer and signaling gradients between aggregates, which results in heterogeneous differentiation and therefore variability in product quality and yield. We have characterized a chemical‐based method to control aggregate size within a specific, tunable range with low heterogeneity, thereby reducing process variability in PSC expansion. This method enables controlled, scalable, stirred suspension‐based manufacturing of PSC cultures that are critical for the translation of regenerative medicine strategies to clinical products.
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spelling pubmed-60557172018-07-23 Chemically controlled aggregation of pluripotent stem cells Lipsitz, Yonatan Y. Tonge, Peter D. Zandstra, Peter W. Biotechnol Bioeng COMMUNICATION TO THE EDITOR Heterogeneity in pluripotent stem cell (PSC) aggregation leads to variability in mass transfer and signaling gradients between aggregates, which results in heterogeneous differentiation and therefore variability in product quality and yield. We have characterized a chemical‐based method to control aggregate size within a specific, tunable range with low heterogeneity, thereby reducing process variability in PSC expansion. This method enables controlled, scalable, stirred suspension‐based manufacturing of PSC cultures that are critical for the translation of regenerative medicine strategies to clinical products. John Wiley and Sons Inc. 2018-05-01 2018-08 /pmc/articles/PMC6055717/ /pubmed/29679475 http://dx.doi.org/10.1002/bit.26719 Text en © 2018 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle COMMUNICATION TO THE EDITOR
Lipsitz, Yonatan Y.
Tonge, Peter D.
Zandstra, Peter W.
Chemically controlled aggregation of pluripotent stem cells
title Chemically controlled aggregation of pluripotent stem cells
title_full Chemically controlled aggregation of pluripotent stem cells
title_fullStr Chemically controlled aggregation of pluripotent stem cells
title_full_unstemmed Chemically controlled aggregation of pluripotent stem cells
title_short Chemically controlled aggregation of pluripotent stem cells
title_sort chemically controlled aggregation of pluripotent stem cells
topic COMMUNICATION TO THE EDITOR
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6055717/
https://www.ncbi.nlm.nih.gov/pubmed/29679475
http://dx.doi.org/10.1002/bit.26719
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