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Chemically controlled aggregation of pluripotent stem cells
Heterogeneity in pluripotent stem cell (PSC) aggregation leads to variability in mass transfer and signaling gradients between aggregates, which results in heterogeneous differentiation and therefore variability in product quality and yield. We have characterized a chemical‐based method to control a...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6055717/ https://www.ncbi.nlm.nih.gov/pubmed/29679475 http://dx.doi.org/10.1002/bit.26719 |
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author | Lipsitz, Yonatan Y. Tonge, Peter D. Zandstra, Peter W. |
author_facet | Lipsitz, Yonatan Y. Tonge, Peter D. Zandstra, Peter W. |
author_sort | Lipsitz, Yonatan Y. |
collection | PubMed |
description | Heterogeneity in pluripotent stem cell (PSC) aggregation leads to variability in mass transfer and signaling gradients between aggregates, which results in heterogeneous differentiation and therefore variability in product quality and yield. We have characterized a chemical‐based method to control aggregate size within a specific, tunable range with low heterogeneity, thereby reducing process variability in PSC expansion. This method enables controlled, scalable, stirred suspension‐based manufacturing of PSC cultures that are critical for the translation of regenerative medicine strategies to clinical products. |
format | Online Article Text |
id | pubmed-6055717 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60557172018-07-23 Chemically controlled aggregation of pluripotent stem cells Lipsitz, Yonatan Y. Tonge, Peter D. Zandstra, Peter W. Biotechnol Bioeng COMMUNICATION TO THE EDITOR Heterogeneity in pluripotent stem cell (PSC) aggregation leads to variability in mass transfer and signaling gradients between aggregates, which results in heterogeneous differentiation and therefore variability in product quality and yield. We have characterized a chemical‐based method to control aggregate size within a specific, tunable range with low heterogeneity, thereby reducing process variability in PSC expansion. This method enables controlled, scalable, stirred suspension‐based manufacturing of PSC cultures that are critical for the translation of regenerative medicine strategies to clinical products. John Wiley and Sons Inc. 2018-05-01 2018-08 /pmc/articles/PMC6055717/ /pubmed/29679475 http://dx.doi.org/10.1002/bit.26719 Text en © 2018 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | COMMUNICATION TO THE EDITOR Lipsitz, Yonatan Y. Tonge, Peter D. Zandstra, Peter W. Chemically controlled aggregation of pluripotent stem cells |
title | Chemically controlled aggregation of pluripotent stem cells |
title_full | Chemically controlled aggregation of pluripotent stem cells |
title_fullStr | Chemically controlled aggregation of pluripotent stem cells |
title_full_unstemmed | Chemically controlled aggregation of pluripotent stem cells |
title_short | Chemically controlled aggregation of pluripotent stem cells |
title_sort | chemically controlled aggregation of pluripotent stem cells |
topic | COMMUNICATION TO THE EDITOR |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6055717/ https://www.ncbi.nlm.nih.gov/pubmed/29679475 http://dx.doi.org/10.1002/bit.26719 |
work_keys_str_mv | AT lipsitzyonatany chemicallycontrolledaggregationofpluripotentstemcells AT tongepeterd chemicallycontrolledaggregationofpluripotentstemcells AT zandstrapeterw chemicallycontrolledaggregationofpluripotentstemcells |