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Low-glycosylated forms of both FSH and LH play major roles in the natural ovarian stimulation

BACKGROUND: The natural ovarian stimulation is mediated by four gonadotrophin glycoforms: FSHtri with three, FSHtetra with four, LHdi with two, and LHtri with three N-glycans. The aim of the study was to determine the serum concentrations of the four glycoforms and their contents of anionic monosacc...

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Autores principales: Wide, Leif, Eriksson, Karin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6055748/
https://www.ncbi.nlm.nih.gov/pubmed/29894213
http://dx.doi.org/10.1080/03009734.2018.1467983
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author Wide, Leif
Eriksson, Karin
author_facet Wide, Leif
Eriksson, Karin
author_sort Wide, Leif
collection PubMed
description BACKGROUND: The natural ovarian stimulation is mediated by four gonadotrophin glycoforms: FSHtri with three, FSHtetra with four, LHdi with two, and LHtri with three N-glycans. The aim of the study was to determine the serum concentrations of the four glycoforms and their contents of anionic monosaccharides (AMS), i.e. sialic acid (SA) and sulfonated N-acetylgalactosamine (SU) residues throughout the menstrual cycle. METHODS: Serum samples were collected from 78 healthy women with regular menstrual cycles. The serum glycoform molecules were identified by their distributions at electrophoreses. Analyses were also performed after removal of terminal SA. The hormones were measured with time-resolved sandwich fluoroimmunoassays. RESULTS: The concentration profiles of the four glycoforms were markedly different. FSHtri, which had a 3-fold higher biopotency than FSHtetra, had peak levels on cycle day 5 and at midcycle and nadirs on cycle days 9 and 21–23. FSHtetra had a raised level on cycle days 5–12, followed by a decrease. LHdi and LHtri had similar patterns, but the peak/nadir ratio was much more pronounced for LHdi than for LHtri, 18 versus 4. The numbers of SA residues per molecule were at a maximum around midcycle when the corresponding numbers of SU were at a minimum. The SU/SA ratio was at a minimum on cycle day 12. CONCLUSION: The results indicate that the LHdi and the FSHtri molecules play major roles in the natural ovarian stimulation. The SU/SA ratios per molecule favoured a prolonged circulatory half-life of all glycoforms at the midcycle phase. The observations may lead to more successful inductions of ovulation in anovulatory women.
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spelling pubmed-60557482018-07-24 Low-glycosylated forms of both FSH and LH play major roles in the natural ovarian stimulation Wide, Leif Eriksson, Karin Ups J Med Sci Articles BACKGROUND: The natural ovarian stimulation is mediated by four gonadotrophin glycoforms: FSHtri with three, FSHtetra with four, LHdi with two, and LHtri with three N-glycans. The aim of the study was to determine the serum concentrations of the four glycoforms and their contents of anionic monosaccharides (AMS), i.e. sialic acid (SA) and sulfonated N-acetylgalactosamine (SU) residues throughout the menstrual cycle. METHODS: Serum samples were collected from 78 healthy women with regular menstrual cycles. The serum glycoform molecules were identified by their distributions at electrophoreses. Analyses were also performed after removal of terminal SA. The hormones were measured with time-resolved sandwich fluoroimmunoassays. RESULTS: The concentration profiles of the four glycoforms were markedly different. FSHtri, which had a 3-fold higher biopotency than FSHtetra, had peak levels on cycle day 5 and at midcycle and nadirs on cycle days 9 and 21–23. FSHtetra had a raised level on cycle days 5–12, followed by a decrease. LHdi and LHtri had similar patterns, but the peak/nadir ratio was much more pronounced for LHdi than for LHtri, 18 versus 4. The numbers of SA residues per molecule were at a maximum around midcycle when the corresponding numbers of SU were at a minimum. The SU/SA ratio was at a minimum on cycle day 12. CONCLUSION: The results indicate that the LHdi and the FSHtri molecules play major roles in the natural ovarian stimulation. The SU/SA ratios per molecule favoured a prolonged circulatory half-life of all glycoforms at the midcycle phase. The observations may lead to more successful inductions of ovulation in anovulatory women. Taylor & Francis 2018-06 2018-06-12 /pmc/articles/PMC6055748/ /pubmed/29894213 http://dx.doi.org/10.1080/03009734.2018.1467983 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Wide, Leif
Eriksson, Karin
Low-glycosylated forms of both FSH and LH play major roles in the natural ovarian stimulation
title Low-glycosylated forms of both FSH and LH play major roles in the natural ovarian stimulation
title_full Low-glycosylated forms of both FSH and LH play major roles in the natural ovarian stimulation
title_fullStr Low-glycosylated forms of both FSH and LH play major roles in the natural ovarian stimulation
title_full_unstemmed Low-glycosylated forms of both FSH and LH play major roles in the natural ovarian stimulation
title_short Low-glycosylated forms of both FSH and LH play major roles in the natural ovarian stimulation
title_sort low-glycosylated forms of both fsh and lh play major roles in the natural ovarian stimulation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6055748/
https://www.ncbi.nlm.nih.gov/pubmed/29894213
http://dx.doi.org/10.1080/03009734.2018.1467983
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