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Arabidopsis Lunapark proteins are involved in ER cisternae formation

The plant endoplasmic reticulum (ER) is crucial to the maintenance of cellular homeostasis. The ER consists of a dynamic and continuously remodelling network of tubules and cisternae. Several conserved membrane proteins have been implicated in formation and maintenance of the ER network in plants, s...

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Autores principales: Kriechbaumer, Verena, Breeze, Emily, Pain, Charlotte, Tolmie, Frances, Frigerio, Lorenzo, Hawes, Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6055799/
https://www.ncbi.nlm.nih.gov/pubmed/29797722
http://dx.doi.org/10.1111/nph.15228
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author Kriechbaumer, Verena
Breeze, Emily
Pain, Charlotte
Tolmie, Frances
Frigerio, Lorenzo
Hawes, Chris
author_facet Kriechbaumer, Verena
Breeze, Emily
Pain, Charlotte
Tolmie, Frances
Frigerio, Lorenzo
Hawes, Chris
author_sort Kriechbaumer, Verena
collection PubMed
description The plant endoplasmic reticulum (ER) is crucial to the maintenance of cellular homeostasis. The ER consists of a dynamic and continuously remodelling network of tubules and cisternae. Several conserved membrane proteins have been implicated in formation and maintenance of the ER network in plants, such as RHD3 and the reticulon proteins. Despite the recent work in mammalian and yeast cells, the detailed molecular mechanisms of ER network organization in plants remain largely unknown. Recently, novel ER network‐shaping proteins called Lunapark (LNP) have been identified in yeast and mammalian cells. Here we identify two Arabidopsis LNP homologues and investigate their subcellular localization via confocal microscopy and potential function in shaping the ER network using protein–protein interaction assays and mutant analysis. We show that AtLNP1 overexpression in tobacco leaf epidermal cells mainly labels cisternae in the ER network, whereas AtLNP2 labels the whole ER. Overexpression of LNP proteins results in an increased abundance of ER cisternae and lnp1 and lnp1lnp2 amiRNA lines display a reduction in cisternae and larger polygonal areas. Thus, we hypothesize that AtLNP1 and AtLNP2 are involved in determining the network morphology of the plant ER, possibly by regulating the formation of ER cisternae.
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spelling pubmed-60557992018-07-30 Arabidopsis Lunapark proteins are involved in ER cisternae formation Kriechbaumer, Verena Breeze, Emily Pain, Charlotte Tolmie, Frances Frigerio, Lorenzo Hawes, Chris New Phytol Research The plant endoplasmic reticulum (ER) is crucial to the maintenance of cellular homeostasis. The ER consists of a dynamic and continuously remodelling network of tubules and cisternae. Several conserved membrane proteins have been implicated in formation and maintenance of the ER network in plants, such as RHD3 and the reticulon proteins. Despite the recent work in mammalian and yeast cells, the detailed molecular mechanisms of ER network organization in plants remain largely unknown. Recently, novel ER network‐shaping proteins called Lunapark (LNP) have been identified in yeast and mammalian cells. Here we identify two Arabidopsis LNP homologues and investigate their subcellular localization via confocal microscopy and potential function in shaping the ER network using protein–protein interaction assays and mutant analysis. We show that AtLNP1 overexpression in tobacco leaf epidermal cells mainly labels cisternae in the ER network, whereas AtLNP2 labels the whole ER. Overexpression of LNP proteins results in an increased abundance of ER cisternae and lnp1 and lnp1lnp2 amiRNA lines display a reduction in cisternae and larger polygonal areas. Thus, we hypothesize that AtLNP1 and AtLNP2 are involved in determining the network morphology of the plant ER, possibly by regulating the formation of ER cisternae. John Wiley and Sons Inc. 2018-05-25 2018-08 /pmc/articles/PMC6055799/ /pubmed/29797722 http://dx.doi.org/10.1111/nph.15228 Text en © 2018 The Authors. New Phytologist © 2018 New Phytologist Trust This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Kriechbaumer, Verena
Breeze, Emily
Pain, Charlotte
Tolmie, Frances
Frigerio, Lorenzo
Hawes, Chris
Arabidopsis Lunapark proteins are involved in ER cisternae formation
title Arabidopsis Lunapark proteins are involved in ER cisternae formation
title_full Arabidopsis Lunapark proteins are involved in ER cisternae formation
title_fullStr Arabidopsis Lunapark proteins are involved in ER cisternae formation
title_full_unstemmed Arabidopsis Lunapark proteins are involved in ER cisternae formation
title_short Arabidopsis Lunapark proteins are involved in ER cisternae formation
title_sort arabidopsis lunapark proteins are involved in er cisternae formation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6055799/
https://www.ncbi.nlm.nih.gov/pubmed/29797722
http://dx.doi.org/10.1111/nph.15228
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