Cargando…

Volatile compounds emission from teratogenic human pluripotent stem cells observed during their differentiation in vivo

Several investigations point out that the volatile fraction of metabolites, often called volatilome, might signal the difference processes occurring in living beings, both in vitro and in vivo. These studies have been recently applied to stem cells biology, and preliminary results show that the comp...

Descripción completa

Detalles Bibliográficos
Autores principales: Capuano, Rosamaria, Spitalieri, Paola, Talarico, Rosa Valentina, Catini, Alexandro, Domakoski, Ana Carolina, Martinelli, Eugenio, Scioli, Maria Giovanna, Orlandi, Augusto, Cicconi, Rosella, Paolesse, Roberto, Novelli, Giuseppe, Di Natale, Corrado, Sangiuolo, Federica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056464/
https://www.ncbi.nlm.nih.gov/pubmed/30038375
http://dx.doi.org/10.1038/s41598-018-29212-0
_version_ 1783341340970450944
author Capuano, Rosamaria
Spitalieri, Paola
Talarico, Rosa Valentina
Catini, Alexandro
Domakoski, Ana Carolina
Martinelli, Eugenio
Scioli, Maria Giovanna
Orlandi, Augusto
Cicconi, Rosella
Paolesse, Roberto
Novelli, Giuseppe
Di Natale, Corrado
Sangiuolo, Federica
author_facet Capuano, Rosamaria
Spitalieri, Paola
Talarico, Rosa Valentina
Catini, Alexandro
Domakoski, Ana Carolina
Martinelli, Eugenio
Scioli, Maria Giovanna
Orlandi, Augusto
Cicconi, Rosella
Paolesse, Roberto
Novelli, Giuseppe
Di Natale, Corrado
Sangiuolo, Federica
author_sort Capuano, Rosamaria
collection PubMed
description Several investigations point out that the volatile fraction of metabolites, often called volatilome, might signal the difference processes occurring in living beings, both in vitro and in vivo. These studies have been recently applied to stem cells biology, and preliminary results show that the composition of the volatilome of stem cells in vitro changes along the differentiation processes leading from pluripotency to full differentiation. The identification of pluripotent stem cells is of great importance to improve safety in regenerative medicine avoiding the formation of teratomas. In this paper, we applied gas chromatography and gas sensor array to the study of the volatilome released by mice transplanted with human induced pluripotent stem cells (hiPSCs) or embryoid bodies (EBs) derived from hiPSCs at 5 days and spontaneously differentiated cells at 27 day. Gas chromatography analysis finds that, in mice transplanted with hiPSCs, the abundance of 13 volatile compounds increases four weeks after the implant and immediately before the formation of malignant teratomas (grade 3) become observable. The same behaviour is also followed by the signals of the gas sensors. Besides this event, the gas-chromatograms and the sensors signals do not show any appreciable variation related neither among the groups of transplanted mice nor respect to a placebo population. This is the first in vivo observation of the change of volatile metabolites released by human induced pluripotent stem cells and hiPSCs-derived cells during the differentiation process. These results shed further light on the differentiation mechanisms of stem cells and suggest possible applications for diagnostic purposes for an early detection of tumor relapse after surgery.
format Online
Article
Text
id pubmed-6056464
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-60564642018-07-30 Volatile compounds emission from teratogenic human pluripotent stem cells observed during their differentiation in vivo Capuano, Rosamaria Spitalieri, Paola Talarico, Rosa Valentina Catini, Alexandro Domakoski, Ana Carolina Martinelli, Eugenio Scioli, Maria Giovanna Orlandi, Augusto Cicconi, Rosella Paolesse, Roberto Novelli, Giuseppe Di Natale, Corrado Sangiuolo, Federica Sci Rep Article Several investigations point out that the volatile fraction of metabolites, often called volatilome, might signal the difference processes occurring in living beings, both in vitro and in vivo. These studies have been recently applied to stem cells biology, and preliminary results show that the composition of the volatilome of stem cells in vitro changes along the differentiation processes leading from pluripotency to full differentiation. The identification of pluripotent stem cells is of great importance to improve safety in regenerative medicine avoiding the formation of teratomas. In this paper, we applied gas chromatography and gas sensor array to the study of the volatilome released by mice transplanted with human induced pluripotent stem cells (hiPSCs) or embryoid bodies (EBs) derived from hiPSCs at 5 days and spontaneously differentiated cells at 27 day. Gas chromatography analysis finds that, in mice transplanted with hiPSCs, the abundance of 13 volatile compounds increases four weeks after the implant and immediately before the formation of malignant teratomas (grade 3) become observable. The same behaviour is also followed by the signals of the gas sensors. Besides this event, the gas-chromatograms and the sensors signals do not show any appreciable variation related neither among the groups of transplanted mice nor respect to a placebo population. This is the first in vivo observation of the change of volatile metabolites released by human induced pluripotent stem cells and hiPSCs-derived cells during the differentiation process. These results shed further light on the differentiation mechanisms of stem cells and suggest possible applications for diagnostic purposes for an early detection of tumor relapse after surgery. Nature Publishing Group UK 2018-07-23 /pmc/articles/PMC6056464/ /pubmed/30038375 http://dx.doi.org/10.1038/s41598-018-29212-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Capuano, Rosamaria
Spitalieri, Paola
Talarico, Rosa Valentina
Catini, Alexandro
Domakoski, Ana Carolina
Martinelli, Eugenio
Scioli, Maria Giovanna
Orlandi, Augusto
Cicconi, Rosella
Paolesse, Roberto
Novelli, Giuseppe
Di Natale, Corrado
Sangiuolo, Federica
Volatile compounds emission from teratogenic human pluripotent stem cells observed during their differentiation in vivo
title Volatile compounds emission from teratogenic human pluripotent stem cells observed during their differentiation in vivo
title_full Volatile compounds emission from teratogenic human pluripotent stem cells observed during their differentiation in vivo
title_fullStr Volatile compounds emission from teratogenic human pluripotent stem cells observed during their differentiation in vivo
title_full_unstemmed Volatile compounds emission from teratogenic human pluripotent stem cells observed during their differentiation in vivo
title_short Volatile compounds emission from teratogenic human pluripotent stem cells observed during their differentiation in vivo
title_sort volatile compounds emission from teratogenic human pluripotent stem cells observed during their differentiation in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056464/
https://www.ncbi.nlm.nih.gov/pubmed/30038375
http://dx.doi.org/10.1038/s41598-018-29212-0
work_keys_str_mv AT capuanorosamaria volatilecompoundsemissionfromteratogenichumanpluripotentstemcellsobservedduringtheirdifferentiationinvivo
AT spitalieripaola volatilecompoundsemissionfromteratogenichumanpluripotentstemcellsobservedduringtheirdifferentiationinvivo
AT talaricorosavalentina volatilecompoundsemissionfromteratogenichumanpluripotentstemcellsobservedduringtheirdifferentiationinvivo
AT catinialexandro volatilecompoundsemissionfromteratogenichumanpluripotentstemcellsobservedduringtheirdifferentiationinvivo
AT domakoskianacarolina volatilecompoundsemissionfromteratogenichumanpluripotentstemcellsobservedduringtheirdifferentiationinvivo
AT martinellieugenio volatilecompoundsemissionfromteratogenichumanpluripotentstemcellsobservedduringtheirdifferentiationinvivo
AT sciolimariagiovanna volatilecompoundsemissionfromteratogenichumanpluripotentstemcellsobservedduringtheirdifferentiationinvivo
AT orlandiaugusto volatilecompoundsemissionfromteratogenichumanpluripotentstemcellsobservedduringtheirdifferentiationinvivo
AT cicconirosella volatilecompoundsemissionfromteratogenichumanpluripotentstemcellsobservedduringtheirdifferentiationinvivo
AT paolesseroberto volatilecompoundsemissionfromteratogenichumanpluripotentstemcellsobservedduringtheirdifferentiationinvivo
AT novelligiuseppe volatilecompoundsemissionfromteratogenichumanpluripotentstemcellsobservedduringtheirdifferentiationinvivo
AT dinatalecorrado volatilecompoundsemissionfromteratogenichumanpluripotentstemcellsobservedduringtheirdifferentiationinvivo
AT sangiuolofederica volatilecompoundsemissionfromteratogenichumanpluripotentstemcellsobservedduringtheirdifferentiationinvivo