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A temporal shift of the evolutionary principle shaping intratumor heterogeneity in colorectal cancer

Advanced colorectal cancer harbors extensive intratumor heterogeneity shaped by neutral evolution; however, intratumor heterogeneity in colorectal precancerous lesions has been poorly studied. We perform multiregion whole-exome sequencing on ten early colorectal tumors, which contained adenoma and c...

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Autores principales: Saito, Tomoko, Niida, Atsushi, Uchi, Ryutaro, Hirata, Hidenari, Komatsu, Hisateru, Sakimura, Shotaro, Hayashi, Shuto, Nambara, Sho, Kuroda, Yosuke, Ito, Shuhei, Eguchi, Hidetoshi, Masuda, Takaaki, Sugimachi, Keishi, Tobo, Taro, Nishida, Haruto, Daa, Tsutomu, Chiba, Kenichi, Shiraishi, Yuichi, Yoshizato, Tetsuichi, Kodama, Masaaki, Okimoto, Tadayoshi, Mizukami, Kazuhiro, Ogawa, Ryo, Okamoto, Kazuhisa, Shuto, Mitsutaka, Fukuda, Kensuke, Matsui, Yusuke, Shimamura, Teppei, Hasegawa, Takanori, Doki, Yuichiro, Nagayama, Satoshi, Yamada, Kazutaka, Kato, Mamoru, Shibata, Tatsuhiro, Mori, Masaki, Aburatani, Hiroyuki, Murakami, Kazunari, Suzuki, Yutaka, Ogawa, Seishi, Miyano, Satoru, Mimori, Koshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056524/
https://www.ncbi.nlm.nih.gov/pubmed/30038269
http://dx.doi.org/10.1038/s41467-018-05226-0
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author Saito, Tomoko
Niida, Atsushi
Uchi, Ryutaro
Hirata, Hidenari
Komatsu, Hisateru
Sakimura, Shotaro
Hayashi, Shuto
Nambara, Sho
Kuroda, Yosuke
Ito, Shuhei
Eguchi, Hidetoshi
Masuda, Takaaki
Sugimachi, Keishi
Tobo, Taro
Nishida, Haruto
Daa, Tsutomu
Chiba, Kenichi
Shiraishi, Yuichi
Yoshizato, Tetsuichi
Kodama, Masaaki
Okimoto, Tadayoshi
Mizukami, Kazuhiro
Ogawa, Ryo
Okamoto, Kazuhisa
Shuto, Mitsutaka
Fukuda, Kensuke
Matsui, Yusuke
Shimamura, Teppei
Hasegawa, Takanori
Doki, Yuichiro
Nagayama, Satoshi
Yamada, Kazutaka
Kato, Mamoru
Shibata, Tatsuhiro
Mori, Masaki
Aburatani, Hiroyuki
Murakami, Kazunari
Suzuki, Yutaka
Ogawa, Seishi
Miyano, Satoru
Mimori, Koshi
author_facet Saito, Tomoko
Niida, Atsushi
Uchi, Ryutaro
Hirata, Hidenari
Komatsu, Hisateru
Sakimura, Shotaro
Hayashi, Shuto
Nambara, Sho
Kuroda, Yosuke
Ito, Shuhei
Eguchi, Hidetoshi
Masuda, Takaaki
Sugimachi, Keishi
Tobo, Taro
Nishida, Haruto
Daa, Tsutomu
Chiba, Kenichi
Shiraishi, Yuichi
Yoshizato, Tetsuichi
Kodama, Masaaki
Okimoto, Tadayoshi
Mizukami, Kazuhiro
Ogawa, Ryo
Okamoto, Kazuhisa
Shuto, Mitsutaka
Fukuda, Kensuke
Matsui, Yusuke
Shimamura, Teppei
Hasegawa, Takanori
Doki, Yuichiro
Nagayama, Satoshi
Yamada, Kazutaka
Kato, Mamoru
Shibata, Tatsuhiro
Mori, Masaki
Aburatani, Hiroyuki
Murakami, Kazunari
Suzuki, Yutaka
Ogawa, Seishi
Miyano, Satoru
Mimori, Koshi
author_sort Saito, Tomoko
collection PubMed
description Advanced colorectal cancer harbors extensive intratumor heterogeneity shaped by neutral evolution; however, intratumor heterogeneity in colorectal precancerous lesions has been poorly studied. We perform multiregion whole-exome sequencing on ten early colorectal tumors, which contained adenoma and carcinoma in situ. By comparing with sequencing data from advanced colorectal tumors, we show that the early tumors accumulate a higher proportion of subclonal driver mutations than the advanced tumors, which is highlighted by subclonal mutations in KRAS and APC. We also demonstrate that variant allele frequencies of subclonal mutations tend to be higher in early tumors, suggesting that the subclonal mutations are subject to selective sweep in early tumorigenesis while neutral evolution is dominant in advanced ones. This study establishes that the evolutionary principle underlying intratumor heterogeneity shifts from Darwinian to neutral evolution during colorectal tumor progression.
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spelling pubmed-60565242018-07-26 A temporal shift of the evolutionary principle shaping intratumor heterogeneity in colorectal cancer Saito, Tomoko Niida, Atsushi Uchi, Ryutaro Hirata, Hidenari Komatsu, Hisateru Sakimura, Shotaro Hayashi, Shuto Nambara, Sho Kuroda, Yosuke Ito, Shuhei Eguchi, Hidetoshi Masuda, Takaaki Sugimachi, Keishi Tobo, Taro Nishida, Haruto Daa, Tsutomu Chiba, Kenichi Shiraishi, Yuichi Yoshizato, Tetsuichi Kodama, Masaaki Okimoto, Tadayoshi Mizukami, Kazuhiro Ogawa, Ryo Okamoto, Kazuhisa Shuto, Mitsutaka Fukuda, Kensuke Matsui, Yusuke Shimamura, Teppei Hasegawa, Takanori Doki, Yuichiro Nagayama, Satoshi Yamada, Kazutaka Kato, Mamoru Shibata, Tatsuhiro Mori, Masaki Aburatani, Hiroyuki Murakami, Kazunari Suzuki, Yutaka Ogawa, Seishi Miyano, Satoru Mimori, Koshi Nat Commun Article Advanced colorectal cancer harbors extensive intratumor heterogeneity shaped by neutral evolution; however, intratumor heterogeneity in colorectal precancerous lesions has been poorly studied. We perform multiregion whole-exome sequencing on ten early colorectal tumors, which contained adenoma and carcinoma in situ. By comparing with sequencing data from advanced colorectal tumors, we show that the early tumors accumulate a higher proportion of subclonal driver mutations than the advanced tumors, which is highlighted by subclonal mutations in KRAS and APC. We also demonstrate that variant allele frequencies of subclonal mutations tend to be higher in early tumors, suggesting that the subclonal mutations are subject to selective sweep in early tumorigenesis while neutral evolution is dominant in advanced ones. This study establishes that the evolutionary principle underlying intratumor heterogeneity shifts from Darwinian to neutral evolution during colorectal tumor progression. Nature Publishing Group UK 2018-07-23 /pmc/articles/PMC6056524/ /pubmed/30038269 http://dx.doi.org/10.1038/s41467-018-05226-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Saito, Tomoko
Niida, Atsushi
Uchi, Ryutaro
Hirata, Hidenari
Komatsu, Hisateru
Sakimura, Shotaro
Hayashi, Shuto
Nambara, Sho
Kuroda, Yosuke
Ito, Shuhei
Eguchi, Hidetoshi
Masuda, Takaaki
Sugimachi, Keishi
Tobo, Taro
Nishida, Haruto
Daa, Tsutomu
Chiba, Kenichi
Shiraishi, Yuichi
Yoshizato, Tetsuichi
Kodama, Masaaki
Okimoto, Tadayoshi
Mizukami, Kazuhiro
Ogawa, Ryo
Okamoto, Kazuhisa
Shuto, Mitsutaka
Fukuda, Kensuke
Matsui, Yusuke
Shimamura, Teppei
Hasegawa, Takanori
Doki, Yuichiro
Nagayama, Satoshi
Yamada, Kazutaka
Kato, Mamoru
Shibata, Tatsuhiro
Mori, Masaki
Aburatani, Hiroyuki
Murakami, Kazunari
Suzuki, Yutaka
Ogawa, Seishi
Miyano, Satoru
Mimori, Koshi
A temporal shift of the evolutionary principle shaping intratumor heterogeneity in colorectal cancer
title A temporal shift of the evolutionary principle shaping intratumor heterogeneity in colorectal cancer
title_full A temporal shift of the evolutionary principle shaping intratumor heterogeneity in colorectal cancer
title_fullStr A temporal shift of the evolutionary principle shaping intratumor heterogeneity in colorectal cancer
title_full_unstemmed A temporal shift of the evolutionary principle shaping intratumor heterogeneity in colorectal cancer
title_short A temporal shift of the evolutionary principle shaping intratumor heterogeneity in colorectal cancer
title_sort temporal shift of the evolutionary principle shaping intratumor heterogeneity in colorectal cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056524/
https://www.ncbi.nlm.nih.gov/pubmed/30038269
http://dx.doi.org/10.1038/s41467-018-05226-0
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