When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing

Here, we sequenced DNA extracted from a necrotic hepatic lesion from a patient with suspected chronic hepatic brucelloma but negative culture results. Although most of the taxonomically classified sequencing reads corresponded to human genome sequences, our data suggest that whole-metagenome shotgun...

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Autores principales: Lazarevic, Vladimir, Gaïa, Nadia, Girard, Myriam, Leo, Stefano, Cherkaoui, Abdessalam, Renzi, Gesuele, Emonet, Stéphane, Jamme, Sharon, Ruppé, Etienne, Vijgen, Sandrine, Rubbia-Brandt, Laura, Toso, Christian, Schrenzel, Jacques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056729/
https://www.ncbi.nlm.nih.gov/pubmed/30065706
http://dx.doi.org/10.3389/fmicb.2018.01566
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author Lazarevic, Vladimir
Gaïa, Nadia
Girard, Myriam
Leo, Stefano
Cherkaoui, Abdessalam
Renzi, Gesuele
Emonet, Stéphane
Jamme, Sharon
Ruppé, Etienne
Vijgen, Sandrine
Rubbia-Brandt, Laura
Toso, Christian
Schrenzel, Jacques
author_facet Lazarevic, Vladimir
Gaïa, Nadia
Girard, Myriam
Leo, Stefano
Cherkaoui, Abdessalam
Renzi, Gesuele
Emonet, Stéphane
Jamme, Sharon
Ruppé, Etienne
Vijgen, Sandrine
Rubbia-Brandt, Laura
Toso, Christian
Schrenzel, Jacques
author_sort Lazarevic, Vladimir
collection PubMed
description Here, we sequenced DNA extracted from a necrotic hepatic lesion from a patient with suspected chronic hepatic brucelloma but negative culture results. Although most of the taxonomically classified sequencing reads corresponded to human genome sequences, our data suggest that whole-metagenome shotgun sequencing may be used together with other tests to strengthen the diagnosis of hepatic brucelloma.
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spelling pubmed-60567292018-07-31 When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing Lazarevic, Vladimir Gaïa, Nadia Girard, Myriam Leo, Stefano Cherkaoui, Abdessalam Renzi, Gesuele Emonet, Stéphane Jamme, Sharon Ruppé, Etienne Vijgen, Sandrine Rubbia-Brandt, Laura Toso, Christian Schrenzel, Jacques Front Microbiol Microbiology Here, we sequenced DNA extracted from a necrotic hepatic lesion from a patient with suspected chronic hepatic brucelloma but negative culture results. Although most of the taxonomically classified sequencing reads corresponded to human genome sequences, our data suggest that whole-metagenome shotgun sequencing may be used together with other tests to strengthen the diagnosis of hepatic brucelloma. Frontiers Media S.A. 2018-07-17 /pmc/articles/PMC6056729/ /pubmed/30065706 http://dx.doi.org/10.3389/fmicb.2018.01566 Text en Copyright © 2018 Lazarevic, Gaïa, Girard, Leo, Cherkaoui, Renzi, Emonet, Jamme, Ruppé, Vijgen, Rubbia-Brandt, Toso and Schrenzel. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lazarevic, Vladimir
Gaïa, Nadia
Girard, Myriam
Leo, Stefano
Cherkaoui, Abdessalam
Renzi, Gesuele
Emonet, Stéphane
Jamme, Sharon
Ruppé, Etienne
Vijgen, Sandrine
Rubbia-Brandt, Laura
Toso, Christian
Schrenzel, Jacques
When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing
title When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing
title_full When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing
title_fullStr When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing
title_full_unstemmed When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing
title_short When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing
title_sort when bacterial culture fails, metagenomics can help: a case of chronic hepatic brucelloma assessed by next-generation sequencing
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056729/
https://www.ncbi.nlm.nih.gov/pubmed/30065706
http://dx.doi.org/10.3389/fmicb.2018.01566
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