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bHLH13 Regulates Jasmonate-Mediated Defense Responses and Growth

Jasmonates (JAs) regulate plant growth and defense responses. On perception of bioactive JAs, the JA receptor CORONATINE INSENSITIVE1 (COI1) recruits JA ZIM-domain (JAZ) proteins for degradation, and JAZ-targeted transcription factors are released to regulate JA responses. The subgroup IIId bHLH tra...

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Autores principales: Huang, Huang, Gao, Hua, Liu, Bei, Fan, Meng, Wang, Jiaojiao, Wang, Cuili, Tian, Haixia, Wang, Lanxiang, Xie, Chengyuan, Wu, Dewei, Liu, Liangyu, Yan, Jianbin, Qi, Tiancong, Song, Susheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056788/
https://www.ncbi.nlm.nih.gov/pubmed/30046236
http://dx.doi.org/10.1177/1176934318790265
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author Huang, Huang
Gao, Hua
Liu, Bei
Fan, Meng
Wang, Jiaojiao
Wang, Cuili
Tian, Haixia
Wang, Lanxiang
Xie, Chengyuan
Wu, Dewei
Liu, Liangyu
Yan, Jianbin
Qi, Tiancong
Song, Susheng
author_facet Huang, Huang
Gao, Hua
Liu, Bei
Fan, Meng
Wang, Jiaojiao
Wang, Cuili
Tian, Haixia
Wang, Lanxiang
Xie, Chengyuan
Wu, Dewei
Liu, Liangyu
Yan, Jianbin
Qi, Tiancong
Song, Susheng
author_sort Huang, Huang
collection PubMed
description Jasmonates (JAs) regulate plant growth and defense responses. On perception of bioactive JAs, the JA receptor CORONATINE INSENSITIVE1 (COI1) recruits JA ZIM-domain (JAZ) proteins for degradation, and JAZ-targeted transcription factors are released to regulate JA responses. The subgroup IIId bHLH transcriptional factors, including bHLH17, bHLH13, bHLH3, and bHLH14, interact with JAZs and repress JA responses. In this study, we show that IIId bHLH factors form dimers via the C-terminus in yeast. N-terminus of bHLH13 is essential for its transcriptional repression function. bHLH13 overexpression inhibits Arabidopsis resistance to the necrotrophic fungi Botrytis cinerea and defense against the insect Spodoptera exigua. COI1 mutation disrupts the oversensitivity of the quadruple mutant bhlh3 bhlh13 bhlh14 bhlh17 in various JA responses, including anthocyanin accumulation, root growth inhibition, and defense against B cinerea and S exigua. Disruption of the TTG1/bHLH/MYB complex blocks anthocyanin accumulation of bhlh3 bhlh13 bhlh14 bhlh17, whereas abolishment of MYC2 attenuates JA-inhibitory root growth of bhlh3 bhlh13 bhlh14 bhlh17. These results genetically demonstrate that IIId bHLH factors function downstream of COI1 to inhibit distinctive JA responses via antagonizing different transcriptional activators.
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spelling pubmed-60567882018-07-25 bHLH13 Regulates Jasmonate-Mediated Defense Responses and Growth Huang, Huang Gao, Hua Liu, Bei Fan, Meng Wang, Jiaojiao Wang, Cuili Tian, Haixia Wang, Lanxiang Xie, Chengyuan Wu, Dewei Liu, Liangyu Yan, Jianbin Qi, Tiancong Song, Susheng Evol Bioinform Online Original Research Jasmonates (JAs) regulate plant growth and defense responses. On perception of bioactive JAs, the JA receptor CORONATINE INSENSITIVE1 (COI1) recruits JA ZIM-domain (JAZ) proteins for degradation, and JAZ-targeted transcription factors are released to regulate JA responses. The subgroup IIId bHLH transcriptional factors, including bHLH17, bHLH13, bHLH3, and bHLH14, interact with JAZs and repress JA responses. In this study, we show that IIId bHLH factors form dimers via the C-terminus in yeast. N-terminus of bHLH13 is essential for its transcriptional repression function. bHLH13 overexpression inhibits Arabidopsis resistance to the necrotrophic fungi Botrytis cinerea and defense against the insect Spodoptera exigua. COI1 mutation disrupts the oversensitivity of the quadruple mutant bhlh3 bhlh13 bhlh14 bhlh17 in various JA responses, including anthocyanin accumulation, root growth inhibition, and defense against B cinerea and S exigua. Disruption of the TTG1/bHLH/MYB complex blocks anthocyanin accumulation of bhlh3 bhlh13 bhlh14 bhlh17, whereas abolishment of MYC2 attenuates JA-inhibitory root growth of bhlh3 bhlh13 bhlh14 bhlh17. These results genetically demonstrate that IIId bHLH factors function downstream of COI1 to inhibit distinctive JA responses via antagonizing different transcriptional activators. SAGE Publications 2018-07-23 /pmc/articles/PMC6056788/ /pubmed/30046236 http://dx.doi.org/10.1177/1176934318790265 Text en © The Author(s) 2018 http://www.creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Research
Huang, Huang
Gao, Hua
Liu, Bei
Fan, Meng
Wang, Jiaojiao
Wang, Cuili
Tian, Haixia
Wang, Lanxiang
Xie, Chengyuan
Wu, Dewei
Liu, Liangyu
Yan, Jianbin
Qi, Tiancong
Song, Susheng
bHLH13 Regulates Jasmonate-Mediated Defense Responses and Growth
title bHLH13 Regulates Jasmonate-Mediated Defense Responses and Growth
title_full bHLH13 Regulates Jasmonate-Mediated Defense Responses and Growth
title_fullStr bHLH13 Regulates Jasmonate-Mediated Defense Responses and Growth
title_full_unstemmed bHLH13 Regulates Jasmonate-Mediated Defense Responses and Growth
title_short bHLH13 Regulates Jasmonate-Mediated Defense Responses and Growth
title_sort bhlh13 regulates jasmonate-mediated defense responses and growth
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056788/
https://www.ncbi.nlm.nih.gov/pubmed/30046236
http://dx.doi.org/10.1177/1176934318790265
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