Cargando…

Genome-Wide Association Study of Haploid Male Fertility in Maize (Zea Mays L.)

Large-scale application of the doubled haploid (DH) technology by in vivo haploid induction has greatly improved the efficiency of maize breeding. While the haploid induction rate and the efficiency of identifying haploid plants have greatly improved in recent years, the low efficiency of doubling o...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Hailin, Li, Guoliang, Würschum, Tobias, Zhang, Yao, Zheng, Debo, Yang, Xiaohong, Li, Jiansheng, Liu, Wenxin, Yan, Jianbing, Chen, Shaojiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057118/
https://www.ncbi.nlm.nih.gov/pubmed/30065732
http://dx.doi.org/10.3389/fpls.2018.00974
_version_ 1783341463321444352
author Ma, Hailin
Li, Guoliang
Würschum, Tobias
Zhang, Yao
Zheng, Debo
Yang, Xiaohong
Li, Jiansheng
Liu, Wenxin
Yan, Jianbing
Chen, Shaojiang
author_facet Ma, Hailin
Li, Guoliang
Würschum, Tobias
Zhang, Yao
Zheng, Debo
Yang, Xiaohong
Li, Jiansheng
Liu, Wenxin
Yan, Jianbing
Chen, Shaojiang
author_sort Ma, Hailin
collection PubMed
description Large-scale application of the doubled haploid (DH) technology by in vivo haploid induction has greatly improved the efficiency of maize breeding. While the haploid induction rate and the efficiency of identifying haploid plants have greatly improved in recent years, the low efficiency of doubling of haploid plants has remained and currently presents the main limitation to maize DH line production. In this study, we aimed to assess the available genetic variation for haploid male fertility (HMF), i.e., the production of fertile pollen on haploid plants, and to investigate the underlying genetic architecture. To this end, a diversity panel of 481 maize inbred lines was crossed with “Mo17” and “Zheng58,” the F(1) hybrids subjected to haploid induction, and resulting haploid plants assessed for male fertility in two environments. Across both genetic backgrounds, we observed a large variation of HMF ranging from zero to ~60%, with a mean of 18%, and a heritability of 0.65. HMF was higher in the “Mo17” than in the “Zheng58” background and the correlation between both genetic backgrounds was 0.68. Genome-wide association mapping identified only few putative QTL that jointly explained 22.5% of the phenotypic variance. With the exception of one association explaining 11.77% of the phenotypic variance, all other putative QTL were of minor importance. A genome-wide prediction approach further corroborated the quantitative nature of HMF in maize. Analysis of the 14 significantly associated SNPs revealed several candidate genes. Collectively, our results illustrate the large variation of HMF that can be exploited for maize DH breeding. Owing to the apparent genetic complexity of this trait, this might best be achieved by rapid recurrent phenotypic selection coupled with marker-assisted selection for individual QTL.
format Online
Article
Text
id pubmed-6057118
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-60571182018-07-31 Genome-Wide Association Study of Haploid Male Fertility in Maize (Zea Mays L.) Ma, Hailin Li, Guoliang Würschum, Tobias Zhang, Yao Zheng, Debo Yang, Xiaohong Li, Jiansheng Liu, Wenxin Yan, Jianbing Chen, Shaojiang Front Plant Sci Plant Science Large-scale application of the doubled haploid (DH) technology by in vivo haploid induction has greatly improved the efficiency of maize breeding. While the haploid induction rate and the efficiency of identifying haploid plants have greatly improved in recent years, the low efficiency of doubling of haploid plants has remained and currently presents the main limitation to maize DH line production. In this study, we aimed to assess the available genetic variation for haploid male fertility (HMF), i.e., the production of fertile pollen on haploid plants, and to investigate the underlying genetic architecture. To this end, a diversity panel of 481 maize inbred lines was crossed with “Mo17” and “Zheng58,” the F(1) hybrids subjected to haploid induction, and resulting haploid plants assessed for male fertility in two environments. Across both genetic backgrounds, we observed a large variation of HMF ranging from zero to ~60%, with a mean of 18%, and a heritability of 0.65. HMF was higher in the “Mo17” than in the “Zheng58” background and the correlation between both genetic backgrounds was 0.68. Genome-wide association mapping identified only few putative QTL that jointly explained 22.5% of the phenotypic variance. With the exception of one association explaining 11.77% of the phenotypic variance, all other putative QTL were of minor importance. A genome-wide prediction approach further corroborated the quantitative nature of HMF in maize. Analysis of the 14 significantly associated SNPs revealed several candidate genes. Collectively, our results illustrate the large variation of HMF that can be exploited for maize DH breeding. Owing to the apparent genetic complexity of this trait, this might best be achieved by rapid recurrent phenotypic selection coupled with marker-assisted selection for individual QTL. Frontiers Media S.A. 2018-07-17 /pmc/articles/PMC6057118/ /pubmed/30065732 http://dx.doi.org/10.3389/fpls.2018.00974 Text en Copyright © 2018 Ma, Li, Würschum, Zhang, Zheng, Yang, Li, Liu, Yan and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ma, Hailin
Li, Guoliang
Würschum, Tobias
Zhang, Yao
Zheng, Debo
Yang, Xiaohong
Li, Jiansheng
Liu, Wenxin
Yan, Jianbing
Chen, Shaojiang
Genome-Wide Association Study of Haploid Male Fertility in Maize (Zea Mays L.)
title Genome-Wide Association Study of Haploid Male Fertility in Maize (Zea Mays L.)
title_full Genome-Wide Association Study of Haploid Male Fertility in Maize (Zea Mays L.)
title_fullStr Genome-Wide Association Study of Haploid Male Fertility in Maize (Zea Mays L.)
title_full_unstemmed Genome-Wide Association Study of Haploid Male Fertility in Maize (Zea Mays L.)
title_short Genome-Wide Association Study of Haploid Male Fertility in Maize (Zea Mays L.)
title_sort genome-wide association study of haploid male fertility in maize (zea mays l.)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057118/
https://www.ncbi.nlm.nih.gov/pubmed/30065732
http://dx.doi.org/10.3389/fpls.2018.00974
work_keys_str_mv AT mahailin genomewideassociationstudyofhaploidmalefertilityinmaizezeamaysl
AT liguoliang genomewideassociationstudyofhaploidmalefertilityinmaizezeamaysl
AT wurschumtobias genomewideassociationstudyofhaploidmalefertilityinmaizezeamaysl
AT zhangyao genomewideassociationstudyofhaploidmalefertilityinmaizezeamaysl
AT zhengdebo genomewideassociationstudyofhaploidmalefertilityinmaizezeamaysl
AT yangxiaohong genomewideassociationstudyofhaploidmalefertilityinmaizezeamaysl
AT lijiansheng genomewideassociationstudyofhaploidmalefertilityinmaizezeamaysl
AT liuwenxin genomewideassociationstudyofhaploidmalefertilityinmaizezeamaysl
AT yanjianbing genomewideassociationstudyofhaploidmalefertilityinmaizezeamaysl
AT chenshaojiang genomewideassociationstudyofhaploidmalefertilityinmaizezeamaysl