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Association of furanone C-30 with biofilm formation & antibiotic resistance in Pseudomonas aeruginosa

BACKGROUND & OBJECTIVES: Pseudomonas aeruginosa is an opportunistic pathogen that can cause nosocomial bloodstream infections in humans. This study was aimed to explore the association of furanone C-30 with biofilm formation, quorum sensing (QS) system and antibiotic resistance in P. aeruginosa....

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Autores principales: Zhao, Jingming, Cheng, Wei, He, Xigang, Liu, Yanli, Li, Ji, Sun, Jiaxing, Li, Jinfeng, Wang, Fangfang, Gao, Yufang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057246/
https://www.ncbi.nlm.nih.gov/pubmed/29998876
http://dx.doi.org/10.4103/ijmr.IJMR_2010_16
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author Zhao, Jingming
Cheng, Wei
He, Xigang
Liu, Yanli
Li, Ji
Sun, Jiaxing
Li, Jinfeng
Wang, Fangfang
Gao, Yufang
author_facet Zhao, Jingming
Cheng, Wei
He, Xigang
Liu, Yanli
Li, Ji
Sun, Jiaxing
Li, Jinfeng
Wang, Fangfang
Gao, Yufang
author_sort Zhao, Jingming
collection PubMed
description BACKGROUND & OBJECTIVES: Pseudomonas aeruginosa is an opportunistic pathogen that can cause nosocomial bloodstream infections in humans. This study was aimed to explore the association of furanone C-30 with biofilm formation, quorum sensing (QS) system and antibiotic resistance in P. aeruginosa. METHODS: An in vitro model of P. aeruginosa bacterial biofilm was established using the standard P. aeruginosa strain (PAO-1). After treatment with 2.5 and 5 μg/ml of furanone C-30, the change of biofilm morphology of PAO-1 was observed, and the expression levels of QS-regulated virulence genes (lasB, rhlA and phzA2), QS receptor genes (lasR, rhlR and pqsR) as well as QS signal molecule synthase genes (lasI, rhlI, pqsE and pqsH) were determined. Besides, the AmpC expression was quantified in planktonic and mature biofilm induced by antibiotics. RESULTS: Furanone C-30 treatment significantly inhibited biofilm formation in a dose-dependent manner. With the increase of furanone C-30 concentration, the expression levels of lasB, rhlA, phzA2, pqsR, lasI, rhlI, pqsE and pqsH significantly decreased in mature biofilm bacteria while the expression levels of lasR and rhlR markedly increased. The AmpC expression was significantly decreased in both planktonic and biofilm bacteria induced by imipenem and ceftazidime. INTERPRETATION & CONCLUSIONS: Furanone C-30 may inhibit biofilm formation and antibiotic resistance in P. aeruginosa through regulating QS genes. The inhibitory effect of furanone C-30 on las system appeared to be stronger than that on rhl system. Further studies need to be done with different strains of P. aeruginosa to confirm our findings.
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spelling pubmed-60572462018-08-08 Association of furanone C-30 with biofilm formation & antibiotic resistance in Pseudomonas aeruginosa Zhao, Jingming Cheng, Wei He, Xigang Liu, Yanli Li, Ji Sun, Jiaxing Li, Jinfeng Wang, Fangfang Gao, Yufang Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Pseudomonas aeruginosa is an opportunistic pathogen that can cause nosocomial bloodstream infections in humans. This study was aimed to explore the association of furanone C-30 with biofilm formation, quorum sensing (QS) system and antibiotic resistance in P. aeruginosa. METHODS: An in vitro model of P. aeruginosa bacterial biofilm was established using the standard P. aeruginosa strain (PAO-1). After treatment with 2.5 and 5 μg/ml of furanone C-30, the change of biofilm morphology of PAO-1 was observed, and the expression levels of QS-regulated virulence genes (lasB, rhlA and phzA2), QS receptor genes (lasR, rhlR and pqsR) as well as QS signal molecule synthase genes (lasI, rhlI, pqsE and pqsH) were determined. Besides, the AmpC expression was quantified in planktonic and mature biofilm induced by antibiotics. RESULTS: Furanone C-30 treatment significantly inhibited biofilm formation in a dose-dependent manner. With the increase of furanone C-30 concentration, the expression levels of lasB, rhlA, phzA2, pqsR, lasI, rhlI, pqsE and pqsH significantly decreased in mature biofilm bacteria while the expression levels of lasR and rhlR markedly increased. The AmpC expression was significantly decreased in both planktonic and biofilm bacteria induced by imipenem and ceftazidime. INTERPRETATION & CONCLUSIONS: Furanone C-30 may inhibit biofilm formation and antibiotic resistance in P. aeruginosa through regulating QS genes. The inhibitory effect of furanone C-30 on las system appeared to be stronger than that on rhl system. Further studies need to be done with different strains of P. aeruginosa to confirm our findings. Medknow Publications & Media Pvt Ltd 2018-04 /pmc/articles/PMC6057246/ /pubmed/29998876 http://dx.doi.org/10.4103/ijmr.IJMR_2010_16 Text en Copyright: © 2018 Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Zhao, Jingming
Cheng, Wei
He, Xigang
Liu, Yanli
Li, Ji
Sun, Jiaxing
Li, Jinfeng
Wang, Fangfang
Gao, Yufang
Association of furanone C-30 with biofilm formation & antibiotic resistance in Pseudomonas aeruginosa
title Association of furanone C-30 with biofilm formation & antibiotic resistance in Pseudomonas aeruginosa
title_full Association of furanone C-30 with biofilm formation & antibiotic resistance in Pseudomonas aeruginosa
title_fullStr Association of furanone C-30 with biofilm formation & antibiotic resistance in Pseudomonas aeruginosa
title_full_unstemmed Association of furanone C-30 with biofilm formation & antibiotic resistance in Pseudomonas aeruginosa
title_short Association of furanone C-30 with biofilm formation & antibiotic resistance in Pseudomonas aeruginosa
title_sort association of furanone c-30 with biofilm formation & antibiotic resistance in pseudomonas aeruginosa
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057246/
https://www.ncbi.nlm.nih.gov/pubmed/29998876
http://dx.doi.org/10.4103/ijmr.IJMR_2010_16
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