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Mouse mammary tumour virus-like env nucleotide and p14 signal peptide are present in feline mammary carcinomas, but not in neoplastic or dysplastic canine mammary lesions

Mouse mammary tumour virus-like (MMTV-like) is suspected to be involved in human breast cancer and it has been hypothesized that companion animals might have a role in viral transmission. The aim of our study was to investigate the presence of MMTV-like nucleotide sequences and viral protein in a la...

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Autores principales: Civita, Prospero, Menicagli, Michele, Scopelliti, Claudia, Lessi, Francesca, Millanta, Francesca, Borsacchi, Sara, Parisi, Francesca, Freer, Giulia, Pistello, Mauro, Mazzanti, Chiara Maria, Poli, Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057629/
https://www.ncbi.nlm.nih.gov/pubmed/30040851
http://dx.doi.org/10.1371/journal.pone.0200839
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author Civita, Prospero
Menicagli, Michele
Scopelliti, Claudia
Lessi, Francesca
Millanta, Francesca
Borsacchi, Sara
Parisi, Francesca
Freer, Giulia
Pistello, Mauro
Mazzanti, Chiara Maria
Poli, Alessandro
author_facet Civita, Prospero
Menicagli, Michele
Scopelliti, Claudia
Lessi, Francesca
Millanta, Francesca
Borsacchi, Sara
Parisi, Francesca
Freer, Giulia
Pistello, Mauro
Mazzanti, Chiara Maria
Poli, Alessandro
author_sort Civita, Prospero
collection PubMed
description Mouse mammary tumour virus-like (MMTV-like) is suspected to be involved in human breast cancer and it has been hypothesized that companion animals might have a role in viral transmission. The aim of our study was to investigate the presence of MMTV-like nucleotide sequences and viral protein in a larger number of feline (FMCs) and canine mammary carcinomas (CMCs) by nested PCR and immunohistochemistry. Results showed that the presence of MMTV-like env sequence in FMCs was 7% (6/86), while all the CMCs and canine dysplastic lesions scored negative. All PCR-positive FMCs scored positive for the MMTV p14 signal peptide of the envelope precursor protein of the virus. In contrast, all PCR-negative FMCs and canine mammary lesions were also negative for immunohistochemistry analysis. Canine and feline normal mammary gland tissues scored negative for both PCR and MMTV-p14 protein. Multiple nucleotide alignment of MMTV-like env gene sequences isolated from cat showed 97% and 99% similarity with HMTV and MMTV, respectively, while the others two presented some polimorphisms. Particularly the sequences of one of these two tumors showed a polymorphism (c.7575 A> G), that causes a previously unreported amino acid substitution (Thr > Ala). In conclusion, the results of our study showed the presence of MMTV-like sequences and viral protein in some FMCs. Further studies are needed to understand whether this virus does play a role in the development of FMCs, if MMTV-like is an exogenous virus as these data suggest and, in such a case, how and from whom this virus was acquired.
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spelling pubmed-60576292018-08-06 Mouse mammary tumour virus-like env nucleotide and p14 signal peptide are present in feline mammary carcinomas, but not in neoplastic or dysplastic canine mammary lesions Civita, Prospero Menicagli, Michele Scopelliti, Claudia Lessi, Francesca Millanta, Francesca Borsacchi, Sara Parisi, Francesca Freer, Giulia Pistello, Mauro Mazzanti, Chiara Maria Poli, Alessandro PLoS One Research Article Mouse mammary tumour virus-like (MMTV-like) is suspected to be involved in human breast cancer and it has been hypothesized that companion animals might have a role in viral transmission. The aim of our study was to investigate the presence of MMTV-like nucleotide sequences and viral protein in a larger number of feline (FMCs) and canine mammary carcinomas (CMCs) by nested PCR and immunohistochemistry. Results showed that the presence of MMTV-like env sequence in FMCs was 7% (6/86), while all the CMCs and canine dysplastic lesions scored negative. All PCR-positive FMCs scored positive for the MMTV p14 signal peptide of the envelope precursor protein of the virus. In contrast, all PCR-negative FMCs and canine mammary lesions were also negative for immunohistochemistry analysis. Canine and feline normal mammary gland tissues scored negative for both PCR and MMTV-p14 protein. Multiple nucleotide alignment of MMTV-like env gene sequences isolated from cat showed 97% and 99% similarity with HMTV and MMTV, respectively, while the others two presented some polimorphisms. Particularly the sequences of one of these two tumors showed a polymorphism (c.7575 A> G), that causes a previously unreported amino acid substitution (Thr > Ala). In conclusion, the results of our study showed the presence of MMTV-like sequences and viral protein in some FMCs. Further studies are needed to understand whether this virus does play a role in the development of FMCs, if MMTV-like is an exogenous virus as these data suggest and, in such a case, how and from whom this virus was acquired. Public Library of Science 2018-07-24 /pmc/articles/PMC6057629/ /pubmed/30040851 http://dx.doi.org/10.1371/journal.pone.0200839 Text en © 2018 Civita et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Civita, Prospero
Menicagli, Michele
Scopelliti, Claudia
Lessi, Francesca
Millanta, Francesca
Borsacchi, Sara
Parisi, Francesca
Freer, Giulia
Pistello, Mauro
Mazzanti, Chiara Maria
Poli, Alessandro
Mouse mammary tumour virus-like env nucleotide and p14 signal peptide are present in feline mammary carcinomas, but not in neoplastic or dysplastic canine mammary lesions
title Mouse mammary tumour virus-like env nucleotide and p14 signal peptide are present in feline mammary carcinomas, but not in neoplastic or dysplastic canine mammary lesions
title_full Mouse mammary tumour virus-like env nucleotide and p14 signal peptide are present in feline mammary carcinomas, but not in neoplastic or dysplastic canine mammary lesions
title_fullStr Mouse mammary tumour virus-like env nucleotide and p14 signal peptide are present in feline mammary carcinomas, but not in neoplastic or dysplastic canine mammary lesions
title_full_unstemmed Mouse mammary tumour virus-like env nucleotide and p14 signal peptide are present in feline mammary carcinomas, but not in neoplastic or dysplastic canine mammary lesions
title_short Mouse mammary tumour virus-like env nucleotide and p14 signal peptide are present in feline mammary carcinomas, but not in neoplastic or dysplastic canine mammary lesions
title_sort mouse mammary tumour virus-like env nucleotide and p14 signal peptide are present in feline mammary carcinomas, but not in neoplastic or dysplastic canine mammary lesions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057629/
https://www.ncbi.nlm.nih.gov/pubmed/30040851
http://dx.doi.org/10.1371/journal.pone.0200839
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