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Production of α-1,3-L-arabinofuranosidase active on substituted xylan does not improve compost degradation by Agaricus bisporus
Agaricus bisporus consumes carbohydrates contained in wheat straw based compost used for commercial mushroom production. Double substituted arabinoxylan is part of the ~40% of the compost polysaccharides that are not degraded by A. bisporus during its growth and development. Genes encoding α-1,3-l-a...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057652/ https://www.ncbi.nlm.nih.gov/pubmed/30040824 http://dx.doi.org/10.1371/journal.pone.0201090 |
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author | Vos, Aurin M. Jurak, Edita de Gijsel, Peter Ohm, Robin A. Henrissat, Bernard Lugones, Luis G. Kabel, Mirjam A. Wösten, Han A. B. |
author_facet | Vos, Aurin M. Jurak, Edita de Gijsel, Peter Ohm, Robin A. Henrissat, Bernard Lugones, Luis G. Kabel, Mirjam A. Wösten, Han A. B. |
author_sort | Vos, Aurin M. |
collection | PubMed |
description | Agaricus bisporus consumes carbohydrates contained in wheat straw based compost used for commercial mushroom production. Double substituted arabinoxylan is part of the ~40% of the compost polysaccharides that are not degraded by A. bisporus during its growth and development. Genes encoding α-1,3-l-arabinofuranosidase (AXHd3) enzymes that act on xylosyl residues doubly substituted with arabinosyl residues are absent in this mushroom forming fungus. Here, the AXHd3 encoding hgh43 gene of Humicola insolens was expressed in A. bisporus with the aim to improve its substrate utilization and mushroom yield. Transformants secreted active AXHd3 in compost as shown by the degradation of double substituted arabinoxylan oligomers in an in vitro assay. However, carbohydrate composition and degree of arabinosyl substitution of arabinoxylans were not affected in compost possibly due to inaccessibility of the doubly substituted xylosyl residues. |
format | Online Article Text |
id | pubmed-6057652 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-60576522018-08-06 Production of α-1,3-L-arabinofuranosidase active on substituted xylan does not improve compost degradation by Agaricus bisporus Vos, Aurin M. Jurak, Edita de Gijsel, Peter Ohm, Robin A. Henrissat, Bernard Lugones, Luis G. Kabel, Mirjam A. Wösten, Han A. B. PLoS One Research Article Agaricus bisporus consumes carbohydrates contained in wheat straw based compost used for commercial mushroom production. Double substituted arabinoxylan is part of the ~40% of the compost polysaccharides that are not degraded by A. bisporus during its growth and development. Genes encoding α-1,3-l-arabinofuranosidase (AXHd3) enzymes that act on xylosyl residues doubly substituted with arabinosyl residues are absent in this mushroom forming fungus. Here, the AXHd3 encoding hgh43 gene of Humicola insolens was expressed in A. bisporus with the aim to improve its substrate utilization and mushroom yield. Transformants secreted active AXHd3 in compost as shown by the degradation of double substituted arabinoxylan oligomers in an in vitro assay. However, carbohydrate composition and degree of arabinosyl substitution of arabinoxylans were not affected in compost possibly due to inaccessibility of the doubly substituted xylosyl residues. Public Library of Science 2018-07-24 /pmc/articles/PMC6057652/ /pubmed/30040824 http://dx.doi.org/10.1371/journal.pone.0201090 Text en © 2018 Vos et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Vos, Aurin M. Jurak, Edita de Gijsel, Peter Ohm, Robin A. Henrissat, Bernard Lugones, Luis G. Kabel, Mirjam A. Wösten, Han A. B. Production of α-1,3-L-arabinofuranosidase active on substituted xylan does not improve compost degradation by Agaricus bisporus |
title | Production of α-1,3-L-arabinofuranosidase active on substituted xylan does not improve compost degradation by Agaricus bisporus |
title_full | Production of α-1,3-L-arabinofuranosidase active on substituted xylan does not improve compost degradation by Agaricus bisporus |
title_fullStr | Production of α-1,3-L-arabinofuranosidase active on substituted xylan does not improve compost degradation by Agaricus bisporus |
title_full_unstemmed | Production of α-1,3-L-arabinofuranosidase active on substituted xylan does not improve compost degradation by Agaricus bisporus |
title_short | Production of α-1,3-L-arabinofuranosidase active on substituted xylan does not improve compost degradation by Agaricus bisporus |
title_sort | production of α-1,3-l-arabinofuranosidase active on substituted xylan does not improve compost degradation by agaricus bisporus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057652/ https://www.ncbi.nlm.nih.gov/pubmed/30040824 http://dx.doi.org/10.1371/journal.pone.0201090 |
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