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Amniotic fluid stem cell models: A tool for filling the gaps in knowledge for human genetic diseases

Induced pluripotent stem (iPS) cells have attracted attention in recent years as a model of human genetic diseases. Starting from the diseased somatic cells isolated from an affected patient, iPS cells can be created and subsequently differentiated into various cell types that can be used to gain a...

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Autores principales: Antonucci, Ivana, Crowley, Marci G., Stuppia, Liborio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057697/
https://www.ncbi.nlm.nih.gov/pubmed/30276320
http://dx.doi.org/10.4103/bc.bc_23_17
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author Antonucci, Ivana
Crowley, Marci G.
Stuppia, Liborio
author_facet Antonucci, Ivana
Crowley, Marci G.
Stuppia, Liborio
author_sort Antonucci, Ivana
collection PubMed
description Induced pluripotent stem (iPS) cells have attracted attention in recent years as a model of human genetic diseases. Starting from the diseased somatic cells isolated from an affected patient, iPS cells can be created and subsequently differentiated into various cell types that can be used to gain a better understanding of the disease at a cellular and molecular level. There are limitations of iPS cell generation, however, due to low efficiency, high costs, and lengthy protocols. The use of amniotic fluid stem cells (AFS) presents a worthy alternative as a stem cell source for modeling of human genetic diseases. Prenatal identification of chromosomal or Mendelian diseases may require the collection of amniotic fluid which is not only useful for the sake of diagnosis but also from this, AFS cells can be isolated and cultured. Since AFS cells show some characteristics of pluripotency, having the capacity to differentiate into various cell types derived from all three germ layers in vitro, they are a well-suited model for investigations regarding alterations in the molecular biology of a cell due to a specific genetic disease. This readily accessible source of stem cells can replace the necessity for generating iPS cells. Here, we expand on the applicability and importance of AFS cells as a model for discovery in the field of human genetic disease research. This paper is a review article. Referred literature in this paper has been listed in the references section. The data sets supporting the conclusions of this article are available online by searching various databases, including PubMed. Some original points in this article come from the laboratory practice in our research center and the authors’ experiences.
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spelling pubmed-60576972018-10-01 Amniotic fluid stem cell models: A tool for filling the gaps in knowledge for human genetic diseases Antonucci, Ivana Crowley, Marci G. Stuppia, Liborio Brain Circ Review Article Induced pluripotent stem (iPS) cells have attracted attention in recent years as a model of human genetic diseases. Starting from the diseased somatic cells isolated from an affected patient, iPS cells can be created and subsequently differentiated into various cell types that can be used to gain a better understanding of the disease at a cellular and molecular level. There are limitations of iPS cell generation, however, due to low efficiency, high costs, and lengthy protocols. The use of amniotic fluid stem cells (AFS) presents a worthy alternative as a stem cell source for modeling of human genetic diseases. Prenatal identification of chromosomal or Mendelian diseases may require the collection of amniotic fluid which is not only useful for the sake of diagnosis but also from this, AFS cells can be isolated and cultured. Since AFS cells show some characteristics of pluripotency, having the capacity to differentiate into various cell types derived from all three germ layers in vitro, they are a well-suited model for investigations regarding alterations in the molecular biology of a cell due to a specific genetic disease. This readily accessible source of stem cells can replace the necessity for generating iPS cells. Here, we expand on the applicability and importance of AFS cells as a model for discovery in the field of human genetic disease research. This paper is a review article. Referred literature in this paper has been listed in the references section. The data sets supporting the conclusions of this article are available online by searching various databases, including PubMed. Some original points in this article come from the laboratory practice in our research center and the authors’ experiences. Medknow Publications & Media Pvt Ltd 2017 2017-10-12 /pmc/articles/PMC6057697/ /pubmed/30276320 http://dx.doi.org/10.4103/bc.bc_23_17 Text en Copyright: © 2017 Brain Circulation http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Review Article
Antonucci, Ivana
Crowley, Marci G.
Stuppia, Liborio
Amniotic fluid stem cell models: A tool for filling the gaps in knowledge for human genetic diseases
title Amniotic fluid stem cell models: A tool for filling the gaps in knowledge for human genetic diseases
title_full Amniotic fluid stem cell models: A tool for filling the gaps in knowledge for human genetic diseases
title_fullStr Amniotic fluid stem cell models: A tool for filling the gaps in knowledge for human genetic diseases
title_full_unstemmed Amniotic fluid stem cell models: A tool for filling the gaps in knowledge for human genetic diseases
title_short Amniotic fluid stem cell models: A tool for filling the gaps in knowledge for human genetic diseases
title_sort amniotic fluid stem cell models: a tool for filling the gaps in knowledge for human genetic diseases
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057697/
https://www.ncbi.nlm.nih.gov/pubmed/30276320
http://dx.doi.org/10.4103/bc.bc_23_17
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