Cargando…

Evolutionary cell biology traces the rise of the exomer complex in Fungi from an ancient eukaryotic component

Cargo is transported from the trans-Golgi Network to the plasma membrane by adaptor complexes, which are pan-eukaryotic components. However, in yeast, cargo can also be exported by the exomer complex, a heterotetrameric protein complex consisting of two copies of Chs5, and any two members of four pa...

Descripción completa

Detalles Bibliográficos
Autores principales: Ramirez-Macias, Inmaculada, Barlow, Lael D., Anton, Carlos, Spang, Anne, Roncero, Cesar, Dacks, Joel B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057913/
https://www.ncbi.nlm.nih.gov/pubmed/30042439
http://dx.doi.org/10.1038/s41598-018-29416-4
_version_ 1783341596397273088
author Ramirez-Macias, Inmaculada
Barlow, Lael D.
Anton, Carlos
Spang, Anne
Roncero, Cesar
Dacks, Joel B.
author_facet Ramirez-Macias, Inmaculada
Barlow, Lael D.
Anton, Carlos
Spang, Anne
Roncero, Cesar
Dacks, Joel B.
author_sort Ramirez-Macias, Inmaculada
collection PubMed
description Cargo is transported from the trans-Golgi Network to the plasma membrane by adaptor complexes, which are pan-eukaryotic components. However, in yeast, cargo can also be exported by the exomer complex, a heterotetrameric protein complex consisting of two copies of Chs5, and any two members of four paralogous proteins (ChAPs). To understand the larger relevance of exomer, its phylogenetic distribution and function outside of yeast need to be explored. We find that the four ChAP proteins are derived from gene duplications after the divergence of Yarrowia from the remaining Saccharomycotina, with BC8 paralogues (Bch2 and Chs6) being more diverged relative to the BB8 paralogues (Bch1 and Bud7), suggesting neofunctionalization. Outside Ascomycota, a single preduplicate ChAP is present in nearly all Fungi and in diverse eukaryotes, but has been repeatedly lost. Chs5, however, is a fungal specific feature, appearing coincidentally with the loss of AP-4. In contrast, the ChAP protein is a wide-spread, yet uncharacterized, membrane-trafficking component, adding one more piece to the increasingly complex machinery deduced as being present in our ancient eukaryotic ancestor.
format Online
Article
Text
id pubmed-6057913
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-60579132018-07-31 Evolutionary cell biology traces the rise of the exomer complex in Fungi from an ancient eukaryotic component Ramirez-Macias, Inmaculada Barlow, Lael D. Anton, Carlos Spang, Anne Roncero, Cesar Dacks, Joel B. Sci Rep Article Cargo is transported from the trans-Golgi Network to the plasma membrane by adaptor complexes, which are pan-eukaryotic components. However, in yeast, cargo can also be exported by the exomer complex, a heterotetrameric protein complex consisting of two copies of Chs5, and any two members of four paralogous proteins (ChAPs). To understand the larger relevance of exomer, its phylogenetic distribution and function outside of yeast need to be explored. We find that the four ChAP proteins are derived from gene duplications after the divergence of Yarrowia from the remaining Saccharomycotina, with BC8 paralogues (Bch2 and Chs6) being more diverged relative to the BB8 paralogues (Bch1 and Bud7), suggesting neofunctionalization. Outside Ascomycota, a single preduplicate ChAP is present in nearly all Fungi and in diverse eukaryotes, but has been repeatedly lost. Chs5, however, is a fungal specific feature, appearing coincidentally with the loss of AP-4. In contrast, the ChAP protein is a wide-spread, yet uncharacterized, membrane-trafficking component, adding one more piece to the increasingly complex machinery deduced as being present in our ancient eukaryotic ancestor. Nature Publishing Group UK 2018-07-24 /pmc/articles/PMC6057913/ /pubmed/30042439 http://dx.doi.org/10.1038/s41598-018-29416-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ramirez-Macias, Inmaculada
Barlow, Lael D.
Anton, Carlos
Spang, Anne
Roncero, Cesar
Dacks, Joel B.
Evolutionary cell biology traces the rise of the exomer complex in Fungi from an ancient eukaryotic component
title Evolutionary cell biology traces the rise of the exomer complex in Fungi from an ancient eukaryotic component
title_full Evolutionary cell biology traces the rise of the exomer complex in Fungi from an ancient eukaryotic component
title_fullStr Evolutionary cell biology traces the rise of the exomer complex in Fungi from an ancient eukaryotic component
title_full_unstemmed Evolutionary cell biology traces the rise of the exomer complex in Fungi from an ancient eukaryotic component
title_short Evolutionary cell biology traces the rise of the exomer complex in Fungi from an ancient eukaryotic component
title_sort evolutionary cell biology traces the rise of the exomer complex in fungi from an ancient eukaryotic component
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057913/
https://www.ncbi.nlm.nih.gov/pubmed/30042439
http://dx.doi.org/10.1038/s41598-018-29416-4
work_keys_str_mv AT ramirezmaciasinmaculada evolutionarycellbiologytracestheriseoftheexomercomplexinfungifromanancienteukaryoticcomponent
AT barlowlaeld evolutionarycellbiologytracestheriseoftheexomercomplexinfungifromanancienteukaryoticcomponent
AT antoncarlos evolutionarycellbiologytracestheriseoftheexomercomplexinfungifromanancienteukaryoticcomponent
AT spanganne evolutionarycellbiologytracestheriseoftheexomercomplexinfungifromanancienteukaryoticcomponent
AT roncerocesar evolutionarycellbiologytracestheriseoftheexomercomplexinfungifromanancienteukaryoticcomponent
AT dacksjoelb evolutionarycellbiologytracestheriseoftheexomercomplexinfungifromanancienteukaryoticcomponent