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LncRNA MIR100HG promotes cell proliferation in triple-negative breast cancer through triplex formation with p27 loci
Triple-negative breast cancer (TNBC) exhibits poor prognosis, with high metastasis and low survival. Long non-coding RNAs (lncRNAs) play critical roles in tumor progression. Here, we identified lncRNA MIR100HG as a pro-oncogene for TNBC progression. Knockdown of MIR100HG decreased cell proliferation...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057987/ https://www.ncbi.nlm.nih.gov/pubmed/30042378 http://dx.doi.org/10.1038/s41419-018-0869-2 |
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author | Wang, Shaowei Ke, Hao Zhang, Honglei Ma, Yujie Ao, Lei Zou, Li Yang, Qin Zhu, Hao Nie, Jianyun Wu, Chunlian Jiao, Baowei |
author_facet | Wang, Shaowei Ke, Hao Zhang, Honglei Ma, Yujie Ao, Lei Zou, Li Yang, Qin Zhu, Hao Nie, Jianyun Wu, Chunlian Jiao, Baowei |
author_sort | Wang, Shaowei |
collection | PubMed |
description | Triple-negative breast cancer (TNBC) exhibits poor prognosis, with high metastasis and low survival. Long non-coding RNAs (lncRNAs) play critical roles in tumor progression. Here, we identified lncRNA MIR100HG as a pro-oncogene for TNBC progression. Knockdown of MIR100HG decreased cell proliferation and induced cell arrest in the G1 phase, whereas overexpression of MIR100HG significantly increased cell proliferation. Furthermore, MIR100HG regulated the p27 gene to control the cell cycle, and subsequently impacted the progression of TNBC. In analyzing its underlying mechanism, bioinformatics prediction and experimental data demonstrated that MIR100HG participated in the formation of RNA–DNA triplex structures. MIR100HG in The Cancer Genome Atlas (TCGA) and breast cancer cell lines showed higher expression in TNBC than in other tumor types with poor prognosis. In conclusion, our data indicated a novel working pattern of lncRNA in TNBC progression, which may be a potential therapeutic target in such cancers. |
format | Online Article Text |
id | pubmed-6057987 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60579872018-07-27 LncRNA MIR100HG promotes cell proliferation in triple-negative breast cancer through triplex formation with p27 loci Wang, Shaowei Ke, Hao Zhang, Honglei Ma, Yujie Ao, Lei Zou, Li Yang, Qin Zhu, Hao Nie, Jianyun Wu, Chunlian Jiao, Baowei Cell Death Dis Article Triple-negative breast cancer (TNBC) exhibits poor prognosis, with high metastasis and low survival. Long non-coding RNAs (lncRNAs) play critical roles in tumor progression. Here, we identified lncRNA MIR100HG as a pro-oncogene for TNBC progression. Knockdown of MIR100HG decreased cell proliferation and induced cell arrest in the G1 phase, whereas overexpression of MIR100HG significantly increased cell proliferation. Furthermore, MIR100HG regulated the p27 gene to control the cell cycle, and subsequently impacted the progression of TNBC. In analyzing its underlying mechanism, bioinformatics prediction and experimental data demonstrated that MIR100HG participated in the formation of RNA–DNA triplex structures. MIR100HG in The Cancer Genome Atlas (TCGA) and breast cancer cell lines showed higher expression in TNBC than in other tumor types with poor prognosis. In conclusion, our data indicated a novel working pattern of lncRNA in TNBC progression, which may be a potential therapeutic target in such cancers. Nature Publishing Group UK 2018-07-24 /pmc/articles/PMC6057987/ /pubmed/30042378 http://dx.doi.org/10.1038/s41419-018-0869-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wang, Shaowei Ke, Hao Zhang, Honglei Ma, Yujie Ao, Lei Zou, Li Yang, Qin Zhu, Hao Nie, Jianyun Wu, Chunlian Jiao, Baowei LncRNA MIR100HG promotes cell proliferation in triple-negative breast cancer through triplex formation with p27 loci |
title | LncRNA MIR100HG promotes cell proliferation in triple-negative breast cancer through triplex formation with p27 loci |
title_full | LncRNA MIR100HG promotes cell proliferation in triple-negative breast cancer through triplex formation with p27 loci |
title_fullStr | LncRNA MIR100HG promotes cell proliferation in triple-negative breast cancer through triplex formation with p27 loci |
title_full_unstemmed | LncRNA MIR100HG promotes cell proliferation in triple-negative breast cancer through triplex formation with p27 loci |
title_short | LncRNA MIR100HG promotes cell proliferation in triple-negative breast cancer through triplex formation with p27 loci |
title_sort | lncrna mir100hg promotes cell proliferation in triple-negative breast cancer through triplex formation with p27 loci |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057987/ https://www.ncbi.nlm.nih.gov/pubmed/30042378 http://dx.doi.org/10.1038/s41419-018-0869-2 |
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