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DNA Flow cytometric analysis of the human testicular tissues to investigate the status of spermatogenesis in azoospermic patients

A single, rapid and reproducible diagnostic test to predict the type of azoospermia and outcome of sperm retrieval is not yet available. So the feasibility of employing DNA flow cytometry for rapid investigation of the status of spermatogenesis in the patients with azoospermia was investigated. Test...

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Autores principales: Baksi, Arka, Vasan, S. S., Dighe, Rajan R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057995/
https://www.ncbi.nlm.nih.gov/pubmed/30042518
http://dx.doi.org/10.1038/s41598-018-29369-8
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author Baksi, Arka
Vasan, S. S.
Dighe, Rajan R.
author_facet Baksi, Arka
Vasan, S. S.
Dighe, Rajan R.
author_sort Baksi, Arka
collection PubMed
description A single, rapid and reproducible diagnostic test to predict the type of azoospermia and outcome of sperm retrieval is not yet available. So the feasibility of employing DNA flow cytometry for rapid investigation of the status of spermatogenesis in the patients with azoospermia was investigated. Testicular biopsies of 44 patients with azoospermia undergoing sperm-retrieval surgery and 4 controls were analyzed by flow cytometry to ascertain their testicular germ-cell patterns. The observed germ-cell pattern was further confirmed by RT-PCR analysis of the cell-specific markers and histology for some patients. The patients with Obstructive Azoospermia (OA) exhibited normal spermatogenesis similar to the control fertile patients showing the presence of diploid, double-diploid and haploid cells. The non-obstructive azoospermia (NOA) patients exhibited disrupted spermatogenesis with arrest at the pre-meiotic (only diploid cells present) or meiotic (diploid and double-diploid cells present) stages. The germ-cell pattern, as ascertained by flow cytometry, provided a clear picture of the intra-testicular spermatogenesis and the presence of spermatozoa in the patients’ testes, which was prognostic of their sperm-retrieval. DNA flow cytometry test to ascertain the testicular germ-cell pattern is simple in execution, analysis and interpretation, requires small amount of tissue and provides quantitative data about the status of spermatogenesis in patients. This test would allow comparable analysis of the status of spermatogenesis in patients across clinics and may form the basis for deciding future treatment and intervention strategies.
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spelling pubmed-60579952018-07-31 DNA Flow cytometric analysis of the human testicular tissues to investigate the status of spermatogenesis in azoospermic patients Baksi, Arka Vasan, S. S. Dighe, Rajan R. Sci Rep Article A single, rapid and reproducible diagnostic test to predict the type of azoospermia and outcome of sperm retrieval is not yet available. So the feasibility of employing DNA flow cytometry for rapid investigation of the status of spermatogenesis in the patients with azoospermia was investigated. Testicular biopsies of 44 patients with azoospermia undergoing sperm-retrieval surgery and 4 controls were analyzed by flow cytometry to ascertain their testicular germ-cell patterns. The observed germ-cell pattern was further confirmed by RT-PCR analysis of the cell-specific markers and histology for some patients. The patients with Obstructive Azoospermia (OA) exhibited normal spermatogenesis similar to the control fertile patients showing the presence of diploid, double-diploid and haploid cells. The non-obstructive azoospermia (NOA) patients exhibited disrupted spermatogenesis with arrest at the pre-meiotic (only diploid cells present) or meiotic (diploid and double-diploid cells present) stages. The germ-cell pattern, as ascertained by flow cytometry, provided a clear picture of the intra-testicular spermatogenesis and the presence of spermatozoa in the patients’ testes, which was prognostic of their sperm-retrieval. DNA flow cytometry test to ascertain the testicular germ-cell pattern is simple in execution, analysis and interpretation, requires small amount of tissue and provides quantitative data about the status of spermatogenesis in patients. This test would allow comparable analysis of the status of spermatogenesis in patients across clinics and may form the basis for deciding future treatment and intervention strategies. Nature Publishing Group UK 2018-07-24 /pmc/articles/PMC6057995/ /pubmed/30042518 http://dx.doi.org/10.1038/s41598-018-29369-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Baksi, Arka
Vasan, S. S.
Dighe, Rajan R.
DNA Flow cytometric analysis of the human testicular tissues to investigate the status of spermatogenesis in azoospermic patients
title DNA Flow cytometric analysis of the human testicular tissues to investigate the status of spermatogenesis in azoospermic patients
title_full DNA Flow cytometric analysis of the human testicular tissues to investigate the status of spermatogenesis in azoospermic patients
title_fullStr DNA Flow cytometric analysis of the human testicular tissues to investigate the status of spermatogenesis in azoospermic patients
title_full_unstemmed DNA Flow cytometric analysis of the human testicular tissues to investigate the status of spermatogenesis in azoospermic patients
title_short DNA Flow cytometric analysis of the human testicular tissues to investigate the status of spermatogenesis in azoospermic patients
title_sort dna flow cytometric analysis of the human testicular tissues to investigate the status of spermatogenesis in azoospermic patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057995/
https://www.ncbi.nlm.nih.gov/pubmed/30042518
http://dx.doi.org/10.1038/s41598-018-29369-8
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