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Ligand binding studies by high speed centrifugal precipitation and linear spectral summation using ultraviolet–visible absorption spectroscopy
In ligand-protein binding experiments the major challenge is to separate bound from free ligand. Equilibrium and gel filtration separation techniques are often hampered by competition for the ligand and non-specific binding. Biophysical assays have attempted to circumvent this problem using titratio...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6058075/ https://www.ncbi.nlm.nih.gov/pubmed/30050754 http://dx.doi.org/10.1016/j.mex.2018.04.007 |