Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples

BACKGROUND: DNA polymerase β (pol β) is a key enzyme of base excision repair pathway. It is a 1-kb gene consisting of 14 exons. Its catalytic part lies between exon 8 and exon 14. Exon 12 has a role in deoxyribonucleotide triphosphate selection for nucleotide transferase activity. METHODS: Genomic D...

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Autores principales: Khanra, Kalyani, Choudhuri, Indranil, Bhattacharyya, Nandan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pasteur Institute 2018
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6058182/
https://www.ncbi.nlm.nih.gov/pubmed/29524322
http://dx.doi.org/10.29252/ibj.22.5.355
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author Khanra, Kalyani
Choudhuri, Indranil
Bhattacharyya, Nandan
author_facet Khanra, Kalyani
Choudhuri, Indranil
Bhattacharyya, Nandan
author_sort Khanra, Kalyani
collection PubMed
description BACKGROUND: DNA polymerase β (pol β) is a key enzyme of base excision repair pathway. It is a 1-kb gene consisting of 14 exons. Its catalytic part lies between exon 8 and exon 14. Exon 12 has a role in deoxyribonucleotide triphosphate selection for nucleotide transferase activity. METHODS: Genomic DNA was isolated from ovarian carcinoma samples. Single strand conformation polymorphism method was used to detect mutation in genomic DNA. RESULTS: Twenty-four patients of the 152 pair of tumor samples (15.8%) exhibited a point mutation (C→G) in position 725 in exon 12, which shifts proline to arginine (P242R). Statistical analysis showed a significant (p < 0.001) relationship between pol β mutation and the age of detection. CONCLUSION: This is a newly reported somatic mutation of pol β in ovarian carcinoma patients from India.
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spelling pubmed-60581822018-09-01 Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples Khanra, Kalyani Choudhuri, Indranil Bhattacharyya, Nandan Iran Biomed J Short Communication BACKGROUND: DNA polymerase β (pol β) is a key enzyme of base excision repair pathway. It is a 1-kb gene consisting of 14 exons. Its catalytic part lies between exon 8 and exon 14. Exon 12 has a role in deoxyribonucleotide triphosphate selection for nucleotide transferase activity. METHODS: Genomic DNA was isolated from ovarian carcinoma samples. Single strand conformation polymorphism method was used to detect mutation in genomic DNA. RESULTS: Twenty-four patients of the 152 pair of tumor samples (15.8%) exhibited a point mutation (C→G) in position 725 in exon 12, which shifts proline to arginine (P242R). Statistical analysis showed a significant (p < 0.001) relationship between pol β mutation and the age of detection. CONCLUSION: This is a newly reported somatic mutation of pol β in ovarian carcinoma patients from India. Pasteur Institute 2018-09 /pmc/articles/PMC6058182/ /pubmed/29524322 http://dx.doi.org/10.29252/ibj.22.5.355 Text en Copyright: © Iranian Biomedical Journal http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Khanra, Kalyani
Choudhuri, Indranil
Bhattacharyya, Nandan
Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples
title Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples
title_full Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples
title_fullStr Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples
title_full_unstemmed Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples
title_short Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples
title_sort detection of somatic mutation in exon 12 of dna polymerase β in ovarian cancer tissue samples
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6058182/
https://www.ncbi.nlm.nih.gov/pubmed/29524322
http://dx.doi.org/10.29252/ibj.22.5.355
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