Percutaneous Sampling of Local Biomolecule Gradients Across Coronary Artery Atherosclerotic Plaques

A percutaneous catheter device, the Liquid Biopsy System, was developed to sample the unstirred boundary layer of blood upstream and downstream of intact and disrupted human coronary atherosclerotic plaques. Using multiplexed proximity extension assays, release of 20 biomolecules was simultaneously...

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Detalles Bibliográficos
Autores principales: West, Nick E.J., Corrigan, Joseph P., Owen, Richard H.G., Hoole, Stephen P., Brown, Adam J., Blatcher, Stephen, Newby, Andrew C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
CLINICAL RESEARCH
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6058996/
https://www.ncbi.nlm.nih.gov/pubmed/30062180
http://dx.doi.org/10.1016/j.jacbts.2017.07.007
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author West, Nick E.J.
Corrigan, Joseph P.
Owen, Richard H.G.
Hoole, Stephen P.
Brown, Adam J.
Blatcher, Stephen
Newby, Andrew C.
author_facet West, Nick E.J.
Corrigan, Joseph P.
Owen, Richard H.G.
Hoole, Stephen P.
Brown, Adam J.
Blatcher, Stephen
Newby, Andrew C.
author_sort West, Nick E.J.
collection PubMed
description A percutaneous catheter device, the Liquid Biopsy System, was developed to sample the unstirred boundary layer of blood upstream and downstream of intact and disrupted human coronary atherosclerotic plaques. Using multiplexed proximity extension assays, release of 20 biomolecules was simultaneously detected in samples taken across plaques before balloon angioplasty, including the soluble form of the endothelial lectin-like oxidized LDL receptor. Additional biomolecules, including matrix metalloproteinase-12, were released after plaque disruption with angioplasty. These experiments demonstrate the power of the Liquid Biopsy System to yield new scientific insights and its ultimate potential to generate new biomarkers and surrogate endpoints for clinical trials.
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spelling pubmed-60589962018-07-30 Percutaneous Sampling of Local Biomolecule Gradients Across Coronary Artery Atherosclerotic Plaques West, Nick E.J. Corrigan, Joseph P. Owen, Richard H.G. Hoole, Stephen P. Brown, Adam J. Blatcher, Stephen Newby, Andrew C. JACC Basic Transl Sci CLINICAL RESEARCH A percutaneous catheter device, the Liquid Biopsy System, was developed to sample the unstirred boundary layer of blood upstream and downstream of intact and disrupted human coronary atherosclerotic plaques. Using multiplexed proximity extension assays, release of 20 biomolecules was simultaneously detected in samples taken across plaques before balloon angioplasty, including the soluble form of the endothelial lectin-like oxidized LDL receptor. Additional biomolecules, including matrix metalloproteinase-12, were released after plaque disruption with angioplasty. These experiments demonstrate the power of the Liquid Biopsy System to yield new scientific insights and its ultimate potential to generate new biomarkers and surrogate endpoints for clinical trials. Elsevier 2017-12-25 /pmc/articles/PMC6058996/ /pubmed/30062180 http://dx.doi.org/10.1016/j.jacbts.2017.07.007 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle CLINICAL RESEARCH
West, Nick E.J.
Corrigan, Joseph P.
Owen, Richard H.G.
Hoole, Stephen P.
Brown, Adam J.
Blatcher, Stephen
Newby, Andrew C.
Percutaneous Sampling of Local Biomolecule Gradients Across Coronary Artery Atherosclerotic Plaques
title Percutaneous Sampling of Local Biomolecule Gradients Across Coronary Artery Atherosclerotic Plaques
title_full Percutaneous Sampling of Local Biomolecule Gradients Across Coronary Artery Atherosclerotic Plaques
title_fullStr Percutaneous Sampling of Local Biomolecule Gradients Across Coronary Artery Atherosclerotic Plaques
title_full_unstemmed Percutaneous Sampling of Local Biomolecule Gradients Across Coronary Artery Atherosclerotic Plaques
title_short Percutaneous Sampling of Local Biomolecule Gradients Across Coronary Artery Atherosclerotic Plaques
title_sort percutaneous sampling of local biomolecule gradients across coronary artery atherosclerotic plaques
topic CLINICAL RESEARCH
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6058996/
https://www.ncbi.nlm.nih.gov/pubmed/30062180
http://dx.doi.org/10.1016/j.jacbts.2017.07.007
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