Cargando…

Extracellular enzymes of Colletotrichum fructicola  isolates  associated to Apple bitter rot and Glomerella leaf spot

Colletotrichum fructicola causes two important diseases on apple in Southern Brazil, bitter rot (ABR) and Glomerella leaf spot (GLS). In this pathosystem, the Colletotrichum ability to cause different symptoms could be related to differences of extracellular enzymes produced by the fungi. Thus, the...

Descripción completa

Detalles Bibliográficos
Autores principales: Velho, Aline Cristina, Mondino, Pedro, Stadnik, Marciel J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6059057/
https://www.ncbi.nlm.nih.gov/pubmed/30123670
http://dx.doi.org/10.1080/21501203.2018.1464525
_version_ 1783341810310971392
author Velho, Aline Cristina
Mondino, Pedro
Stadnik, Marciel J.
author_facet Velho, Aline Cristina
Mondino, Pedro
Stadnik, Marciel J.
author_sort Velho, Aline Cristina
collection PubMed
description Colletotrichum fructicola causes two important diseases on apple in Southern Brazil, bitter rot (ABR) and Glomerella leaf spot (GLS). In this pathosystem, the Colletotrichum ability to cause different symptoms could be related to differences of extracellular enzymes produced by the fungi. Thus, the objectives of this study were to compare the production of these enzymes between ABR- and GLS-isolate in vitro and to evaluate their involvement on infected apple leaves with C. fructicola. In agar plate enzymatic assay, ABR- showed significantly higher amylolytic and pectolytic activity than GLS-isolate. In contrast, for lipolytic and proteolytic no significant differences were observed between isolates. In culture broth, ABR-isolate also had higher activity of pectin lyase (PNL), polygalacturonase (PG) and laccase (LAC). Notably, LAC was significantly five-fold higher in ABR- than GLS-isolate. On the other hand, in infected apple leaves no significant difference was observed between isolates for PNL, PG and LAC. Although differences in extracellular enzymes of ABR- and GLS-isolate have not been observed in vivo, these results contributed to highlight the importance to investigate such enzymes in depth.
format Online
Article
Text
id pubmed-6059057
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-60590572018-08-17 Extracellular enzymes of Colletotrichum fructicola  isolates  associated to Apple bitter rot and Glomerella leaf spot Velho, Aline Cristina Mondino, Pedro Stadnik, Marciel J. Mycology Article Colletotrichum fructicola causes two important diseases on apple in Southern Brazil, bitter rot (ABR) and Glomerella leaf spot (GLS). In this pathosystem, the Colletotrichum ability to cause different symptoms could be related to differences of extracellular enzymes produced by the fungi. Thus, the objectives of this study were to compare the production of these enzymes between ABR- and GLS-isolate in vitro and to evaluate their involvement on infected apple leaves with C. fructicola. In agar plate enzymatic assay, ABR- showed significantly higher amylolytic and pectolytic activity than GLS-isolate. In contrast, for lipolytic and proteolytic no significant differences were observed between isolates. In culture broth, ABR-isolate also had higher activity of pectin lyase (PNL), polygalacturonase (PG) and laccase (LAC). Notably, LAC was significantly five-fold higher in ABR- than GLS-isolate. On the other hand, in infected apple leaves no significant difference was observed between isolates for PNL, PG and LAC. Although differences in extracellular enzymes of ABR- and GLS-isolate have not been observed in vivo, these results contributed to highlight the importance to investigate such enzymes in depth. Taylor & Francis 2018-04-24 /pmc/articles/PMC6059057/ /pubmed/30123670 http://dx.doi.org/10.1080/21501203.2018.1464525 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Velho, Aline Cristina
Mondino, Pedro
Stadnik, Marciel J.
Extracellular enzymes of Colletotrichum fructicola  isolates  associated to Apple bitter rot and Glomerella leaf spot
title Extracellular enzymes of Colletotrichum fructicola  isolates  associated to Apple bitter rot and Glomerella leaf spot
title_full Extracellular enzymes of Colletotrichum fructicola  isolates  associated to Apple bitter rot and Glomerella leaf spot
title_fullStr Extracellular enzymes of Colletotrichum fructicola  isolates  associated to Apple bitter rot and Glomerella leaf spot
title_full_unstemmed Extracellular enzymes of Colletotrichum fructicola  isolates  associated to Apple bitter rot and Glomerella leaf spot
title_short Extracellular enzymes of Colletotrichum fructicola  isolates  associated to Apple bitter rot and Glomerella leaf spot
title_sort extracellular enzymes of colletotrichum fructicola  isolates  associated to apple bitter rot and glomerella leaf spot
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6059057/
https://www.ncbi.nlm.nih.gov/pubmed/30123670
http://dx.doi.org/10.1080/21501203.2018.1464525
work_keys_str_mv AT velhoalinecristina extracellularenzymesofcolletotrichumfructicolaisolatesassociatedtoapplebitterrotandglomerellaleafspot
AT mondinopedro extracellularenzymesofcolletotrichumfructicolaisolatesassociatedtoapplebitterrotandglomerellaleafspot
AT stadnikmarcielj extracellularenzymesofcolletotrichumfructicolaisolatesassociatedtoapplebitterrotandglomerellaleafspot