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Mesenchymal stem cells derived from human iPS cells via mesoderm and neuroepithelium have different features and therapeutic potentials

Mesenchymal stem cells (MSCs) isolated from adult human tissues are capable of proliferating in vitro and maintaining their multipotency, making them attractive cell sources for regenerative medicine. However, the availability and capability of self-renewal under current preparation regimes are limi...

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Autores principales: Eto, Shinya, Goto, Mizuki, Soga, Minami, Kaneko, Yumi, Uehara, Yusuke, Mizuta, Hiroshi, Era, Takumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6059447/
https://www.ncbi.nlm.nih.gov/pubmed/30044827
http://dx.doi.org/10.1371/journal.pone.0200790
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author Eto, Shinya
Goto, Mizuki
Soga, Minami
Kaneko, Yumi
Uehara, Yusuke
Mizuta, Hiroshi
Era, Takumi
author_facet Eto, Shinya
Goto, Mizuki
Soga, Minami
Kaneko, Yumi
Uehara, Yusuke
Mizuta, Hiroshi
Era, Takumi
author_sort Eto, Shinya
collection PubMed
description Mesenchymal stem cells (MSCs) isolated from adult human tissues are capable of proliferating in vitro and maintaining their multipotency, making them attractive cell sources for regenerative medicine. However, the availability and capability of self-renewal under current preparation regimes are limited. Induced pluripotent stem cells (iPSCs) now offer an alternative, similar cell source to MSCs. Herein, we established new methods for differentiating hiPSCs into MSCs via mesoderm-like and neuroepithelium-like cells. Both derived MSC populations exhibited self-renewal and multipotency, as well as therapeutic potential in mouse models of skin wounds, pressure ulcers, and osteoarthritis. Interestingly, the therapeutic effects differ between the two types of MSCs in the disease models, suggesting that the therapeutic effect depends on the cell origin. Our results provide valuable basic insights for the clinical application of such cells.
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spelling pubmed-60594472018-08-09 Mesenchymal stem cells derived from human iPS cells via mesoderm and neuroepithelium have different features and therapeutic potentials Eto, Shinya Goto, Mizuki Soga, Minami Kaneko, Yumi Uehara, Yusuke Mizuta, Hiroshi Era, Takumi PLoS One Research Article Mesenchymal stem cells (MSCs) isolated from adult human tissues are capable of proliferating in vitro and maintaining their multipotency, making them attractive cell sources for regenerative medicine. However, the availability and capability of self-renewal under current preparation regimes are limited. Induced pluripotent stem cells (iPSCs) now offer an alternative, similar cell source to MSCs. Herein, we established new methods for differentiating hiPSCs into MSCs via mesoderm-like and neuroepithelium-like cells. Both derived MSC populations exhibited self-renewal and multipotency, as well as therapeutic potential in mouse models of skin wounds, pressure ulcers, and osteoarthritis. Interestingly, the therapeutic effects differ between the two types of MSCs in the disease models, suggesting that the therapeutic effect depends on the cell origin. Our results provide valuable basic insights for the clinical application of such cells. Public Library of Science 2018-07-25 /pmc/articles/PMC6059447/ /pubmed/30044827 http://dx.doi.org/10.1371/journal.pone.0200790 Text en © 2018 Eto et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Eto, Shinya
Goto, Mizuki
Soga, Minami
Kaneko, Yumi
Uehara, Yusuke
Mizuta, Hiroshi
Era, Takumi
Mesenchymal stem cells derived from human iPS cells via mesoderm and neuroepithelium have different features and therapeutic potentials
title Mesenchymal stem cells derived from human iPS cells via mesoderm and neuroepithelium have different features and therapeutic potentials
title_full Mesenchymal stem cells derived from human iPS cells via mesoderm and neuroepithelium have different features and therapeutic potentials
title_fullStr Mesenchymal stem cells derived from human iPS cells via mesoderm and neuroepithelium have different features and therapeutic potentials
title_full_unstemmed Mesenchymal stem cells derived from human iPS cells via mesoderm and neuroepithelium have different features and therapeutic potentials
title_short Mesenchymal stem cells derived from human iPS cells via mesoderm and neuroepithelium have different features and therapeutic potentials
title_sort mesenchymal stem cells derived from human ips cells via mesoderm and neuroepithelium have different features and therapeutic potentials
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6059447/
https://www.ncbi.nlm.nih.gov/pubmed/30044827
http://dx.doi.org/10.1371/journal.pone.0200790
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