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PYGB siRNA inhibits the cell proliferation of human osteosarcoma cell lines

Osteosarcoma is the most common malignant bone carcinoma that primarily occurs between childhood to adolescence. It was suggested by recent research that the Brain type glycogen phosphorylase (PYGB) gene may serve an important role in various types of cancer. In the present study, the PYGB gene was...

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Autores principales: Zhang, Shuwei, Zhou, Yichi, Zha, Yuanyu, Yang, Yang, Wang, Linlong, Li, Jingfeng, Jin, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6059700/
https://www.ncbi.nlm.nih.gov/pubmed/29845265
http://dx.doi.org/10.3892/mmr.2018.9022
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author Zhang, Shuwei
Zhou, Yichi
Zha, Yuanyu
Yang, Yang
Wang, Linlong
Li, Jingfeng
Jin, Wei
author_facet Zhang, Shuwei
Zhou, Yichi
Zha, Yuanyu
Yang, Yang
Wang, Linlong
Li, Jingfeng
Jin, Wei
author_sort Zhang, Shuwei
collection PubMed
description Osteosarcoma is the most common malignant bone carcinoma that primarily occurs between childhood to adolescence. It was suggested by recent research that the Brain type glycogen phosphorylase (PYGB) gene may serve an important role in various types of cancer. In the present study, the PYGB gene was knocked down in order to evaluate the cell viability, invasion and migration of the human osteosarcoma cell lines MG63 and HOS. The expression levels of PYGB in osteosarcoma and bone cyst tissue samples, as well as in the osteosarcoma cell lines were identified using reverse transcription-quantitative polymerase chain reaction and western blot assay. Subsequently, a Cell Counting kit 8 assay was employed to evaluate cell proliferation. Cell apoptosis rate and cell cycle distribution were measured by flow cytometry. In addition, cell invasion and migration were evaluated through a Transwell assay. The expression levels of the cell apoptosis and tumor metastasis associated proteins B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein, E-cadherin, Twist, matrix metalloproteinase (MMP)-9 and MMP2 were measured via western blotting. PYGB exhibited a higher expression level in the osteosarcoma tissue samples, particularly in the human osteosarcoma cell lines MG63 and HOS. Knockdown of PYGB resulted in a decline in cell proliferation, invasion and migration, which was coupled with induced cell apoptosis and cell cycle arrest in MG63 and HOS cells. Furthermore, alterations in the expression of apoptosis and metastasis associated proteins indicated that small interfering (si)PYGB may have regulated cell viability by targeting the Bcl/Caspase and cyclin dependent kinase (CDK)-1 signaling pathway. In conclusion, PYGB siRNA exerted an inhibitory effect on the cell viability of the human osteosarcoma cells MG63 and HOS by blocking the Caspase/Bcl and CDK1 signaling pathway, highlighting novel potential therapeutic methods for treating osteosarcoma.
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spelling pubmed-60597002018-07-26 PYGB siRNA inhibits the cell proliferation of human osteosarcoma cell lines Zhang, Shuwei Zhou, Yichi Zha, Yuanyu Yang, Yang Wang, Linlong Li, Jingfeng Jin, Wei Mol Med Rep Articles Osteosarcoma is the most common malignant bone carcinoma that primarily occurs between childhood to adolescence. It was suggested by recent research that the Brain type glycogen phosphorylase (PYGB) gene may serve an important role in various types of cancer. In the present study, the PYGB gene was knocked down in order to evaluate the cell viability, invasion and migration of the human osteosarcoma cell lines MG63 and HOS. The expression levels of PYGB in osteosarcoma and bone cyst tissue samples, as well as in the osteosarcoma cell lines were identified using reverse transcription-quantitative polymerase chain reaction and western blot assay. Subsequently, a Cell Counting kit 8 assay was employed to evaluate cell proliferation. Cell apoptosis rate and cell cycle distribution were measured by flow cytometry. In addition, cell invasion and migration were evaluated through a Transwell assay. The expression levels of the cell apoptosis and tumor metastasis associated proteins B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein, E-cadherin, Twist, matrix metalloproteinase (MMP)-9 and MMP2 were measured via western blotting. PYGB exhibited a higher expression level in the osteosarcoma tissue samples, particularly in the human osteosarcoma cell lines MG63 and HOS. Knockdown of PYGB resulted in a decline in cell proliferation, invasion and migration, which was coupled with induced cell apoptosis and cell cycle arrest in MG63 and HOS cells. Furthermore, alterations in the expression of apoptosis and metastasis associated proteins indicated that small interfering (si)PYGB may have regulated cell viability by targeting the Bcl/Caspase and cyclin dependent kinase (CDK)-1 signaling pathway. In conclusion, PYGB siRNA exerted an inhibitory effect on the cell viability of the human osteosarcoma cells MG63 and HOS by blocking the Caspase/Bcl and CDK1 signaling pathway, highlighting novel potential therapeutic methods for treating osteosarcoma. D.A. Spandidos 2018-07 2018-05-16 /pmc/articles/PMC6059700/ /pubmed/29845265 http://dx.doi.org/10.3892/mmr.2018.9022 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhang, Shuwei
Zhou, Yichi
Zha, Yuanyu
Yang, Yang
Wang, Linlong
Li, Jingfeng
Jin, Wei
PYGB siRNA inhibits the cell proliferation of human osteosarcoma cell lines
title PYGB siRNA inhibits the cell proliferation of human osteosarcoma cell lines
title_full PYGB siRNA inhibits the cell proliferation of human osteosarcoma cell lines
title_fullStr PYGB siRNA inhibits the cell proliferation of human osteosarcoma cell lines
title_full_unstemmed PYGB siRNA inhibits the cell proliferation of human osteosarcoma cell lines
title_short PYGB siRNA inhibits the cell proliferation of human osteosarcoma cell lines
title_sort pygb sirna inhibits the cell proliferation of human osteosarcoma cell lines
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6059700/
https://www.ncbi.nlm.nih.gov/pubmed/29845265
http://dx.doi.org/10.3892/mmr.2018.9022
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