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Integrated bioinformatics analysis reveals that the expression of cathepsin S is associated with lymph node metastasis and poor prognosis in papillary thyroid cancer

The prognosis of the majority of patients with papillary thyroid cancer (PTC) is excellent, although there are patients who experience disease recurrence and progression. The aim of the present study was to identify potential prognostic risk markers in PTC. Differentially expressed genes (DEGs), ide...

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Autores principales: Tan, Juan, Qian, Xiaoxiao, Song, Bin, An, Xiumin, Cai, Tingting, Zuo, Zhihua, Ding, Dafa, Lu, Yibing, Li, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6059735/
https://www.ncbi.nlm.nih.gov/pubmed/29749483
http://dx.doi.org/10.3892/or.2018.6428
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author Tan, Juan
Qian, Xiaoxiao
Song, Bin
An, Xiumin
Cai, Tingting
Zuo, Zhihua
Ding, Dafa
Lu, Yibing
Li, Hong
author_facet Tan, Juan
Qian, Xiaoxiao
Song, Bin
An, Xiumin
Cai, Tingting
Zuo, Zhihua
Ding, Dafa
Lu, Yibing
Li, Hong
author_sort Tan, Juan
collection PubMed
description The prognosis of the majority of patients with papillary thyroid cancer (PTC) is excellent, although there are patients who experience disease recurrence and progression. The aim of the present study was to identify potential prognostic risk markers in PTC. Differentially expressed genes (DEGs), identified from four Genome Expression Omnibus cohorts were subjected to functional enrichment analyses with Gene Ontology terms and the Kyoto Encyclopedia of Genes and Genome pathways. Hub genes, filtered from cytoHubba, were validated using the The Cancer Genome Atlas (TCGA) cohort, and their associations with clinicopathological features and prognosis were analyzed. A total of 277 DEGs were identified following data preprocessing. DEGs were primarily enriched in ‘small cell lung cancer’, ‘ECM-receptor interaction’, ‘pathways in cancer’ and ‘tyrosine metabolism’. Hub genes [APOE, cathepsin S (CTSS), insulin receptor substrate 1 (IRS1), KIT, LGALS3, RUNX2 and TGFBR1] were extracted from cytoHubba. Their expression in the TCGA cohort was consistent with that in the GEO cohorts. CTSS (P=0.006) and IRS1 (P=0.005) were associated with disease-free survival, as determined using the Kaplan-Meier analysis. CTSS was an independent risk factor for poor disease-free survival (HR, 2.649; 95% CI, 1.095–6.409; P=0.031). Patients with high expression of CTSS exhibited different histological types (increased tall-cell subtype and reduced follicular subtype; P<0.001), more frequent lymph node metastasis (P<0.001) and advanced tumor-node-metastasis stages (P=0.049) compared with the low-expression group. High expression of CTSS was independently associated with lymph node metastasis (OR, 2.015; 95% CI, 1.225–3.315; P=0.006). Therefore, CTSS may serve as a predictive risk marker for the progression and prognosis of PTC.
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spelling pubmed-60597352018-07-26 Integrated bioinformatics analysis reveals that the expression of cathepsin S is associated with lymph node metastasis and poor prognosis in papillary thyroid cancer Tan, Juan Qian, Xiaoxiao Song, Bin An, Xiumin Cai, Tingting Zuo, Zhihua Ding, Dafa Lu, Yibing Li, Hong Oncol Rep Articles The prognosis of the majority of patients with papillary thyroid cancer (PTC) is excellent, although there are patients who experience disease recurrence and progression. The aim of the present study was to identify potential prognostic risk markers in PTC. Differentially expressed genes (DEGs), identified from four Genome Expression Omnibus cohorts were subjected to functional enrichment analyses with Gene Ontology terms and the Kyoto Encyclopedia of Genes and Genome pathways. Hub genes, filtered from cytoHubba, were validated using the The Cancer Genome Atlas (TCGA) cohort, and their associations with clinicopathological features and prognosis were analyzed. A total of 277 DEGs were identified following data preprocessing. DEGs were primarily enriched in ‘small cell lung cancer’, ‘ECM-receptor interaction’, ‘pathways in cancer’ and ‘tyrosine metabolism’. Hub genes [APOE, cathepsin S (CTSS), insulin receptor substrate 1 (IRS1), KIT, LGALS3, RUNX2 and TGFBR1] were extracted from cytoHubba. Their expression in the TCGA cohort was consistent with that in the GEO cohorts. CTSS (P=0.006) and IRS1 (P=0.005) were associated with disease-free survival, as determined using the Kaplan-Meier analysis. CTSS was an independent risk factor for poor disease-free survival (HR, 2.649; 95% CI, 1.095–6.409; P=0.031). Patients with high expression of CTSS exhibited different histological types (increased tall-cell subtype and reduced follicular subtype; P<0.001), more frequent lymph node metastasis (P<0.001) and advanced tumor-node-metastasis stages (P=0.049) compared with the low-expression group. High expression of CTSS was independently associated with lymph node metastasis (OR, 2.015; 95% CI, 1.225–3.315; P=0.006). Therefore, CTSS may serve as a predictive risk marker for the progression and prognosis of PTC. D.A. Spandidos 2018-07 2018-05-08 /pmc/articles/PMC6059735/ /pubmed/29749483 http://dx.doi.org/10.3892/or.2018.6428 Text en Copyright: © Tan et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Tan, Juan
Qian, Xiaoxiao
Song, Bin
An, Xiumin
Cai, Tingting
Zuo, Zhihua
Ding, Dafa
Lu, Yibing
Li, Hong
Integrated bioinformatics analysis reveals that the expression of cathepsin S is associated with lymph node metastasis and poor prognosis in papillary thyroid cancer
title Integrated bioinformatics analysis reveals that the expression of cathepsin S is associated with lymph node metastasis and poor prognosis in papillary thyroid cancer
title_full Integrated bioinformatics analysis reveals that the expression of cathepsin S is associated with lymph node metastasis and poor prognosis in papillary thyroid cancer
title_fullStr Integrated bioinformatics analysis reveals that the expression of cathepsin S is associated with lymph node metastasis and poor prognosis in papillary thyroid cancer
title_full_unstemmed Integrated bioinformatics analysis reveals that the expression of cathepsin S is associated with lymph node metastasis and poor prognosis in papillary thyroid cancer
title_short Integrated bioinformatics analysis reveals that the expression of cathepsin S is associated with lymph node metastasis and poor prognosis in papillary thyroid cancer
title_sort integrated bioinformatics analysis reveals that the expression of cathepsin s is associated with lymph node metastasis and poor prognosis in papillary thyroid cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6059735/
https://www.ncbi.nlm.nih.gov/pubmed/29749483
http://dx.doi.org/10.3892/or.2018.6428
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