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βKlotho inhibits androgen/androgen receptor-associated epithelial-mesenchymal transition in prostate cancer through inactivation of ERK1/2 signaling

The epithelial-mesenchymal transition (EMT) is reported to have intimate crosstalk with androgen receptor (AR) signaling in prostate cancer (PCa) and is known to be responsible for castration resistance. Fibroblast growth factor/receptor (FGF/FGFR) signaling is also involved in tumor progression and...

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Autores principales: Liu, Zhao, Zhang, Hui, Ding, Sentai, Qi, Shasha, Liu, Shuai, Sun, Dingqi, Dong, Wei, Yin, Lei, Li, Mingjiang, Zhao, Xingbo, Lu, Jiaju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6059743/
https://www.ncbi.nlm.nih.gov/pubmed/29749458
http://dx.doi.org/10.3892/or.2018.6399
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author Liu, Zhao
Zhang, Hui
Ding, Sentai
Qi, Shasha
Liu, Shuai
Sun, Dingqi
Dong, Wei
Yin, Lei
Li, Mingjiang
Zhao, Xingbo
Lu, Jiaju
author_facet Liu, Zhao
Zhang, Hui
Ding, Sentai
Qi, Shasha
Liu, Shuai
Sun, Dingqi
Dong, Wei
Yin, Lei
Li, Mingjiang
Zhao, Xingbo
Lu, Jiaju
author_sort Liu, Zhao
collection PubMed
description The epithelial-mesenchymal transition (EMT) is reported to have intimate crosstalk with androgen receptor (AR) signaling in prostate cancer (PCa) and is known to be responsible for castration resistance. Fibroblast growth factor/receptor (FGF/FGFR) signaling is also involved in tumor progression and EMT in multiple tissues. Several studies have investigated the role of βKlotho, an FGF/FGFR signaling co-receptor in tumorigenesis. However, its role in PCa remains unknown. In the present study, the role of androgen in the EMT of PCa cells was examined by western blotting. The expression of βKlotho was examined in prostate cells and PCa tissues by western blotting and immunohistochemistry, respectively. The biological role of βKlotho was revealed by a series of functional in vitro and in vivo studies. We determined that βKlotho expression was significantly decreased in PCa tissues compared with benign prostatic hyperplasia (BPH) tissues, and low βKlotho expression was associated with a high Gleason score of PCa. βKlotho overexpression inhibited the viability, migration, and androgen/AR-associated EMT of PCa cells through the inactivation of ERK1/2 signaling. Notably, βKlotho overexpression inhibited prostate tumor growth and EMT in vivo. Knockdown of βKlotho produced the opposite effects. In conclusion, βKlotho inhibits EMT and plays a tumor-suppressive role in PCa, linking FGF/FGFR/βKlotho signaling to the regulation of PCa progression.
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spelling pubmed-60597432018-07-26 βKlotho inhibits androgen/androgen receptor-associated epithelial-mesenchymal transition in prostate cancer through inactivation of ERK1/2 signaling Liu, Zhao Zhang, Hui Ding, Sentai Qi, Shasha Liu, Shuai Sun, Dingqi Dong, Wei Yin, Lei Li, Mingjiang Zhao, Xingbo Lu, Jiaju Oncol Rep Articles The epithelial-mesenchymal transition (EMT) is reported to have intimate crosstalk with androgen receptor (AR) signaling in prostate cancer (PCa) and is known to be responsible for castration resistance. Fibroblast growth factor/receptor (FGF/FGFR) signaling is also involved in tumor progression and EMT in multiple tissues. Several studies have investigated the role of βKlotho, an FGF/FGFR signaling co-receptor in tumorigenesis. However, its role in PCa remains unknown. In the present study, the role of androgen in the EMT of PCa cells was examined by western blotting. The expression of βKlotho was examined in prostate cells and PCa tissues by western blotting and immunohistochemistry, respectively. The biological role of βKlotho was revealed by a series of functional in vitro and in vivo studies. We determined that βKlotho expression was significantly decreased in PCa tissues compared with benign prostatic hyperplasia (BPH) tissues, and low βKlotho expression was associated with a high Gleason score of PCa. βKlotho overexpression inhibited the viability, migration, and androgen/AR-associated EMT of PCa cells through the inactivation of ERK1/2 signaling. Notably, βKlotho overexpression inhibited prostate tumor growth and EMT in vivo. Knockdown of βKlotho produced the opposite effects. In conclusion, βKlotho inhibits EMT and plays a tumor-suppressive role in PCa, linking FGF/FGFR/βKlotho signaling to the regulation of PCa progression. D.A. Spandidos 2018-07 2018-04-25 /pmc/articles/PMC6059743/ /pubmed/29749458 http://dx.doi.org/10.3892/or.2018.6399 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Zhao
Zhang, Hui
Ding, Sentai
Qi, Shasha
Liu, Shuai
Sun, Dingqi
Dong, Wei
Yin, Lei
Li, Mingjiang
Zhao, Xingbo
Lu, Jiaju
βKlotho inhibits androgen/androgen receptor-associated epithelial-mesenchymal transition in prostate cancer through inactivation of ERK1/2 signaling
title βKlotho inhibits androgen/androgen receptor-associated epithelial-mesenchymal transition in prostate cancer through inactivation of ERK1/2 signaling
title_full βKlotho inhibits androgen/androgen receptor-associated epithelial-mesenchymal transition in prostate cancer through inactivation of ERK1/2 signaling
title_fullStr βKlotho inhibits androgen/androgen receptor-associated epithelial-mesenchymal transition in prostate cancer through inactivation of ERK1/2 signaling
title_full_unstemmed βKlotho inhibits androgen/androgen receptor-associated epithelial-mesenchymal transition in prostate cancer through inactivation of ERK1/2 signaling
title_short βKlotho inhibits androgen/androgen receptor-associated epithelial-mesenchymal transition in prostate cancer through inactivation of ERK1/2 signaling
title_sort βklotho inhibits androgen/androgen receptor-associated epithelial-mesenchymal transition in prostate cancer through inactivation of erk1/2 signaling
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6059743/
https://www.ncbi.nlm.nih.gov/pubmed/29749458
http://dx.doi.org/10.3892/or.2018.6399
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