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Non-contact mechanical and chemical analysis of single living cells by microspectroscopic techniques

Innovative label-free microspectroscopy, which can simultaneously collect Brillouin and Raman signals, is used to characterize the viscoelastic properties and chemical composition of living cells with sub-micrometric resolution. The unprecedented statistical accuracy of the data combined with the hi...

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Autores principales: Mattana, Sara, Mattarelli, Maurizio, Urbanelli, Lorena, Sagini, Krizia, Emiliani, Carla, Serra, Mauro Dalla, Fioretto, Daniele, Caponi, Silvia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6060066/
https://www.ncbi.nlm.nih.gov/pubmed/30839528
http://dx.doi.org/10.1038/lsa.2017.139
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author Mattana, Sara
Mattarelli, Maurizio
Urbanelli, Lorena
Sagini, Krizia
Emiliani, Carla
Serra, Mauro Dalla
Fioretto, Daniele
Caponi, Silvia
author_facet Mattana, Sara
Mattarelli, Maurizio
Urbanelli, Lorena
Sagini, Krizia
Emiliani, Carla
Serra, Mauro Dalla
Fioretto, Daniele
Caponi, Silvia
author_sort Mattana, Sara
collection PubMed
description Innovative label-free microspectroscopy, which can simultaneously collect Brillouin and Raman signals, is used to characterize the viscoelastic properties and chemical composition of living cells with sub-micrometric resolution. The unprecedented statistical accuracy of the data combined with the high-frequency resolution and the high contrast of the recently built experimental setup permits the study of single living cells immersed in their buffer solution by contactless measurements. The Brillouin signal is deconvoluted in the buffer and the cell components, thereby revealing the mechanical heterogeneity inside the cell. In particular, a 20% increase is observed in the elastic modulus passing from the plasmatic membrane to the nucleus as distinguished by comparison with the Raman spectroscopic marker. Brillouin line shape analysis is even more relevant for the comparison of cells under physiological and pathological conditions. Following oncogene expression, cells show an overall reduction in the elastic modulus (15%) and apparent viscosity (50%). In a proof-of-principle experiment, the ability of this spectroscopic technique to characterize subcellular compartments and distinguish cell status was successfully tested. The results strongly support the future application of this technique for fundamental issues in the biomedical field.
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spelling pubmed-60600662018-08-30 Non-contact mechanical and chemical analysis of single living cells by microspectroscopic techniques Mattana, Sara Mattarelli, Maurizio Urbanelli, Lorena Sagini, Krizia Emiliani, Carla Serra, Mauro Dalla Fioretto, Daniele Caponi, Silvia Light Sci Appl Article Innovative label-free microspectroscopy, which can simultaneously collect Brillouin and Raman signals, is used to characterize the viscoelastic properties and chemical composition of living cells with sub-micrometric resolution. The unprecedented statistical accuracy of the data combined with the high-frequency resolution and the high contrast of the recently built experimental setup permits the study of single living cells immersed in their buffer solution by contactless measurements. The Brillouin signal is deconvoluted in the buffer and the cell components, thereby revealing the mechanical heterogeneity inside the cell. In particular, a 20% increase is observed in the elastic modulus passing from the plasmatic membrane to the nucleus as distinguished by comparison with the Raman spectroscopic marker. Brillouin line shape analysis is even more relevant for the comparison of cells under physiological and pathological conditions. Following oncogene expression, cells show an overall reduction in the elastic modulus (15%) and apparent viscosity (50%). In a proof-of-principle experiment, the ability of this spectroscopic technique to characterize subcellular compartments and distinguish cell status was successfully tested. The results strongly support the future application of this technique for fundamental issues in the biomedical field. Nature Publishing Group 2018-02-09 /pmc/articles/PMC6060066/ /pubmed/30839528 http://dx.doi.org/10.1038/lsa.2017.139 Text en Copyright © 2018 The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Mattana, Sara
Mattarelli, Maurizio
Urbanelli, Lorena
Sagini, Krizia
Emiliani, Carla
Serra, Mauro Dalla
Fioretto, Daniele
Caponi, Silvia
Non-contact mechanical and chemical analysis of single living cells by microspectroscopic techniques
title Non-contact mechanical and chemical analysis of single living cells by microspectroscopic techniques
title_full Non-contact mechanical and chemical analysis of single living cells by microspectroscopic techniques
title_fullStr Non-contact mechanical and chemical analysis of single living cells by microspectroscopic techniques
title_full_unstemmed Non-contact mechanical and chemical analysis of single living cells by microspectroscopic techniques
title_short Non-contact mechanical and chemical analysis of single living cells by microspectroscopic techniques
title_sort non-contact mechanical and chemical analysis of single living cells by microspectroscopic techniques
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6060066/
https://www.ncbi.nlm.nih.gov/pubmed/30839528
http://dx.doi.org/10.1038/lsa.2017.139
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