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Single nucleotide polymorphism rs11614913 associated with CC genotype in miR-196a2 is overrepresented in laryngeal squamous cell carcinoma, but not salivary gland tumors in Polish population

The miRNA-196a2 has shown significance in the development of various neoplasms, including head and neck squamous cell carcinoma (HNSCC). The oncogenic functionality of this miRNA is mediated via its potential to target annexin A1 mRNA, a tumor suppressor gene involved in inhibition of the NF-κB path...

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Autores principales: Skalski, Marcin, Ustaszewski, Adam, Jaskiewicz, Katarzyna, Kiwerska, Katarzyna, Wierzbicka, Malgorzata, Klimza, Hanna, Grenman, Reidar, Giefing, Maciej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6060993/
https://www.ncbi.nlm.nih.gov/pubmed/29705927
http://dx.doi.org/10.1007/s13353-018-0445-6
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author Skalski, Marcin
Ustaszewski, Adam
Jaskiewicz, Katarzyna
Kiwerska, Katarzyna
Wierzbicka, Malgorzata
Klimza, Hanna
Grenman, Reidar
Giefing, Maciej
author_facet Skalski, Marcin
Ustaszewski, Adam
Jaskiewicz, Katarzyna
Kiwerska, Katarzyna
Wierzbicka, Malgorzata
Klimza, Hanna
Grenman, Reidar
Giefing, Maciej
author_sort Skalski, Marcin
collection PubMed
description The miRNA-196a2 has shown significance in the development of various neoplasms, including head and neck squamous cell carcinoma (HNSCC). The oncogenic functionality of this miRNA is mediated via its potential to target annexin A1 mRNA, a tumor suppressor gene involved in inhibition of the NF-κB pathway. Interestingly, recent data indicate a susceptibility for aforementioned neoplasms in patients with the CC genotype vs the CT and TT genotypes of the rs11614913 SNP located within the DNA sequence of the miR-196a2 that results in elevated expression of the gene. To further investigate this phenomenon, we genotyped this SNP in 40 patients with laryngeal squamous cell carcinoma (LSCC), the most common tumor of the head and neck region and 60 patients with salivary gland tumors (SGT) that show a yet unexplained incidence increase in the last two decades. In agreement with previous reports, we have identified a statistically significant (p < 0.05) overrepresentation of the CC genotype in LSCC patients and demonstrated in LSCC cell lines that it results in elevated expression of miR-196a2 as compared to cell lines with the TT genotype of the respective SNP. Importantly, none of these correlations was found in patients with SGT. These findings underline the importance of the SNP rs11614913 for LSCC development in the Polish population and moreover highlight the different genetic background of the two studied neoplasms of the head and neck region. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13353-018-0445-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-60609932018-08-09 Single nucleotide polymorphism rs11614913 associated with CC genotype in miR-196a2 is overrepresented in laryngeal squamous cell carcinoma, but not salivary gland tumors in Polish population Skalski, Marcin Ustaszewski, Adam Jaskiewicz, Katarzyna Kiwerska, Katarzyna Wierzbicka, Malgorzata Klimza, Hanna Grenman, Reidar Giefing, Maciej J Appl Genet Human Genetics • Short Communication The miRNA-196a2 has shown significance in the development of various neoplasms, including head and neck squamous cell carcinoma (HNSCC). The oncogenic functionality of this miRNA is mediated via its potential to target annexin A1 mRNA, a tumor suppressor gene involved in inhibition of the NF-κB pathway. Interestingly, recent data indicate a susceptibility for aforementioned neoplasms in patients with the CC genotype vs the CT and TT genotypes of the rs11614913 SNP located within the DNA sequence of the miR-196a2 that results in elevated expression of the gene. To further investigate this phenomenon, we genotyped this SNP in 40 patients with laryngeal squamous cell carcinoma (LSCC), the most common tumor of the head and neck region and 60 patients with salivary gland tumors (SGT) that show a yet unexplained incidence increase in the last two decades. In agreement with previous reports, we have identified a statistically significant (p < 0.05) overrepresentation of the CC genotype in LSCC patients and demonstrated in LSCC cell lines that it results in elevated expression of miR-196a2 as compared to cell lines with the TT genotype of the respective SNP. Importantly, none of these correlations was found in patients with SGT. These findings underline the importance of the SNP rs11614913 for LSCC development in the Polish population and moreover highlight the different genetic background of the two studied neoplasms of the head and neck region. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13353-018-0445-6) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-04-29 2018 /pmc/articles/PMC6060993/ /pubmed/29705927 http://dx.doi.org/10.1007/s13353-018-0445-6 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Human Genetics • Short Communication
Skalski, Marcin
Ustaszewski, Adam
Jaskiewicz, Katarzyna
Kiwerska, Katarzyna
Wierzbicka, Malgorzata
Klimza, Hanna
Grenman, Reidar
Giefing, Maciej
Single nucleotide polymorphism rs11614913 associated with CC genotype in miR-196a2 is overrepresented in laryngeal squamous cell carcinoma, but not salivary gland tumors in Polish population
title Single nucleotide polymorphism rs11614913 associated with CC genotype in miR-196a2 is overrepresented in laryngeal squamous cell carcinoma, but not salivary gland tumors in Polish population
title_full Single nucleotide polymorphism rs11614913 associated with CC genotype in miR-196a2 is overrepresented in laryngeal squamous cell carcinoma, but not salivary gland tumors in Polish population
title_fullStr Single nucleotide polymorphism rs11614913 associated with CC genotype in miR-196a2 is overrepresented in laryngeal squamous cell carcinoma, but not salivary gland tumors in Polish population
title_full_unstemmed Single nucleotide polymorphism rs11614913 associated with CC genotype in miR-196a2 is overrepresented in laryngeal squamous cell carcinoma, but not salivary gland tumors in Polish population
title_short Single nucleotide polymorphism rs11614913 associated with CC genotype in miR-196a2 is overrepresented in laryngeal squamous cell carcinoma, but not salivary gland tumors in Polish population
title_sort single nucleotide polymorphism rs11614913 associated with cc genotype in mir-196a2 is overrepresented in laryngeal squamous cell carcinoma, but not salivary gland tumors in polish population
topic Human Genetics • Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6060993/
https://www.ncbi.nlm.nih.gov/pubmed/29705927
http://dx.doi.org/10.1007/s13353-018-0445-6
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