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The prevalence of plasmid-mediated quinolone resistance and ESBL-production in Enterobacteriaceae isolated from urinary tract infections

INTRODUCTION: β-lactam and fluoroquinolone antibiotics are usually used for the treatment of urinary tract infections (UTIs). The aim of this study was to determine the prevalence of plasmid-mediated quinolone resistance (PMQR) and extended spectrum β-lactamases (ESBLs) in Enterobacteriaceae isolate...

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Autores principales: Azargun, Robab, Sadeghi, Mohammad Reza, Soroush Barhaghi, Mohammad Hossein, Samadi Kafil, Hossein, Yeganeh, Fatemeh, Ahangar Oskouee, Mahin, Ghotaslou, Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6061675/
https://www.ncbi.nlm.nih.gov/pubmed/30087570
http://dx.doi.org/10.2147/IDR.S160720
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author Azargun, Robab
Sadeghi, Mohammad Reza
Soroush Barhaghi, Mohammad Hossein
Samadi Kafil, Hossein
Yeganeh, Fatemeh
Ahangar Oskouee, Mahin
Ghotaslou, Reza
author_facet Azargun, Robab
Sadeghi, Mohammad Reza
Soroush Barhaghi, Mohammad Hossein
Samadi Kafil, Hossein
Yeganeh, Fatemeh
Ahangar Oskouee, Mahin
Ghotaslou, Reza
author_sort Azargun, Robab
collection PubMed
description INTRODUCTION: β-lactam and fluoroquinolone antibiotics are usually used for the treatment of urinary tract infections (UTIs). The aim of this study was to determine the prevalence of plasmid-mediated quinolone resistance (PMQR) and extended spectrum β-lactamases (ESBLs) in Enterobacteriaceae isolated from UTIs. MATERIALS AND METHODS: Two hundred and nineteen samples of Enterobacteriaceae isolated from UTIs were collected in the Northwest of Iran. Antimicrobial susceptibility testing was determined by the disk diffusion method. ESBLs were detected by the double-disk test. ESBL and PMQR-encoding genes were screened using the polymerase chain reaction. RESULTS: The rate of resistance to moxifloxacin, nalidixic acid, gatifloxacin, ofloxacin, ciprofloxacin, and levofloxacin in ESBL-producing isolates was 89.3%, 88%, 84%, 80%, 78.7%, and 73.3%, respectively. PMQR-producing Enterobacteriaceae isolates were identified in 67 samples (89.1%). The most prevalent PMQR genes were aac (6′)-Ib-cr 120 (68.6%) followed by oqxB 72 (41.1%), oqxA 59 (33.7%), qnrB 36 (20.6%), qnrS 33 (18.9%), qnrD 19 (10.9%), qepA 13 (7.4 %), qnrA 10 (5.7%), and qnrC 9 (5.1%). There was a strong association between PMQR genes and bla(CTX-M-15) and bla(TEM-116) and other ESBL genes. CONCLUSION: High resistance rates were detected to quinolones among ESBL-producing isolates from UTIs. There is a high prevalence of PMQR genes in Enterobacteriaceae in Azerbaijan and Iran, and the most common PMQR is aac(6′)-Ib-cr. There is a significant association between PMQR and ESBL-producing isolates.
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spelling pubmed-60616752018-08-07 The prevalence of plasmid-mediated quinolone resistance and ESBL-production in Enterobacteriaceae isolated from urinary tract infections Azargun, Robab Sadeghi, Mohammad Reza Soroush Barhaghi, Mohammad Hossein Samadi Kafil, Hossein Yeganeh, Fatemeh Ahangar Oskouee, Mahin Ghotaslou, Reza Infect Drug Resist Original Research INTRODUCTION: β-lactam and fluoroquinolone antibiotics are usually used for the treatment of urinary tract infections (UTIs). The aim of this study was to determine the prevalence of plasmid-mediated quinolone resistance (PMQR) and extended spectrum β-lactamases (ESBLs) in Enterobacteriaceae isolated from UTIs. MATERIALS AND METHODS: Two hundred and nineteen samples of Enterobacteriaceae isolated from UTIs were collected in the Northwest of Iran. Antimicrobial susceptibility testing was determined by the disk diffusion method. ESBLs were detected by the double-disk test. ESBL and PMQR-encoding genes were screened using the polymerase chain reaction. RESULTS: The rate of resistance to moxifloxacin, nalidixic acid, gatifloxacin, ofloxacin, ciprofloxacin, and levofloxacin in ESBL-producing isolates was 89.3%, 88%, 84%, 80%, 78.7%, and 73.3%, respectively. PMQR-producing Enterobacteriaceae isolates were identified in 67 samples (89.1%). The most prevalent PMQR genes were aac (6′)-Ib-cr 120 (68.6%) followed by oqxB 72 (41.1%), oqxA 59 (33.7%), qnrB 36 (20.6%), qnrS 33 (18.9%), qnrD 19 (10.9%), qepA 13 (7.4 %), qnrA 10 (5.7%), and qnrC 9 (5.1%). There was a strong association between PMQR genes and bla(CTX-M-15) and bla(TEM-116) and other ESBL genes. CONCLUSION: High resistance rates were detected to quinolones among ESBL-producing isolates from UTIs. There is a high prevalence of PMQR genes in Enterobacteriaceae in Azerbaijan and Iran, and the most common PMQR is aac(6′)-Ib-cr. There is a significant association between PMQR and ESBL-producing isolates. Dove Medical Press 2018-07-23 /pmc/articles/PMC6061675/ /pubmed/30087570 http://dx.doi.org/10.2147/IDR.S160720 Text en © 2018 Azargun et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Azargun, Robab
Sadeghi, Mohammad Reza
Soroush Barhaghi, Mohammad Hossein
Samadi Kafil, Hossein
Yeganeh, Fatemeh
Ahangar Oskouee, Mahin
Ghotaslou, Reza
The prevalence of plasmid-mediated quinolone resistance and ESBL-production in Enterobacteriaceae isolated from urinary tract infections
title The prevalence of plasmid-mediated quinolone resistance and ESBL-production in Enterobacteriaceae isolated from urinary tract infections
title_full The prevalence of plasmid-mediated quinolone resistance and ESBL-production in Enterobacteriaceae isolated from urinary tract infections
title_fullStr The prevalence of plasmid-mediated quinolone resistance and ESBL-production in Enterobacteriaceae isolated from urinary tract infections
title_full_unstemmed The prevalence of plasmid-mediated quinolone resistance and ESBL-production in Enterobacteriaceae isolated from urinary tract infections
title_short The prevalence of plasmid-mediated quinolone resistance and ESBL-production in Enterobacteriaceae isolated from urinary tract infections
title_sort prevalence of plasmid-mediated quinolone resistance and esbl-production in enterobacteriaceae isolated from urinary tract infections
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6061675/
https://www.ncbi.nlm.nih.gov/pubmed/30087570
http://dx.doi.org/10.2147/IDR.S160720
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