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Investigation of wound healing process guided by nano-scale topographic patterns integrated within a microfluidic system

When living tissues are injured, they undergo a sequential process of homeostasis, inflammation, proliferation and maturation, which is called wound healing. The working mechanism of wound healing has not been wholly understood due to its complex environments with various mechanical and chemical fac...

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Autores principales: Lee, Insu, Kim, Daegyu, Park, Ga-Lahm, Jeon, Tae-Joon, Kim, Sun Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062108/
https://www.ncbi.nlm.nih.gov/pubmed/30048525
http://dx.doi.org/10.1371/journal.pone.0201418
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author Lee, Insu
Kim, Daegyu
Park, Ga-Lahm
Jeon, Tae-Joon
Kim, Sun Min
author_facet Lee, Insu
Kim, Daegyu
Park, Ga-Lahm
Jeon, Tae-Joon
Kim, Sun Min
author_sort Lee, Insu
collection PubMed
description When living tissues are injured, they undergo a sequential process of homeostasis, inflammation, proliferation and maturation, which is called wound healing. The working mechanism of wound healing has not been wholly understood due to its complex environments with various mechanical and chemical factors. In this study, we propose a novel in vitro wound healing model using a microfluidic system that can manipulate the topography of the wound bed. The topography of the extracellular matrix (ECM) in the wound bed is one of the most important mechanical properties for rapid and effective wound healing. We focused our work on the topographical factor which is one of crucial mechanical cues in wound healing process by using various nano-patterns on the cell attachment surface. First, we analyzed the cell morphology and dynamic cellular behaviors of NIH-3T3 fibroblasts on the nano-patterned surface. Their morphology and dynamic behaviors were investigated for relevance with regard to the recovery function. Second, we developed a highly reproducible and inexpensive research platform for wound formation and the wound healing process by combining the nano-patterned surface and a microfluidic channel. The effect of topography on wound recovery performance was analyzed. This in vitro wound healing research platform will provide well-controlled topographic cue of wound bed and contribute to the study on the fundamental mechanism of wound healing.
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spelling pubmed-60621082018-08-03 Investigation of wound healing process guided by nano-scale topographic patterns integrated within a microfluidic system Lee, Insu Kim, Daegyu Park, Ga-Lahm Jeon, Tae-Joon Kim, Sun Min PLoS One Research Article When living tissues are injured, they undergo a sequential process of homeostasis, inflammation, proliferation and maturation, which is called wound healing. The working mechanism of wound healing has not been wholly understood due to its complex environments with various mechanical and chemical factors. In this study, we propose a novel in vitro wound healing model using a microfluidic system that can manipulate the topography of the wound bed. The topography of the extracellular matrix (ECM) in the wound bed is one of the most important mechanical properties for rapid and effective wound healing. We focused our work on the topographical factor which is one of crucial mechanical cues in wound healing process by using various nano-patterns on the cell attachment surface. First, we analyzed the cell morphology and dynamic cellular behaviors of NIH-3T3 fibroblasts on the nano-patterned surface. Their morphology and dynamic behaviors were investigated for relevance with regard to the recovery function. Second, we developed a highly reproducible and inexpensive research platform for wound formation and the wound healing process by combining the nano-patterned surface and a microfluidic channel. The effect of topography on wound recovery performance was analyzed. This in vitro wound healing research platform will provide well-controlled topographic cue of wound bed and contribute to the study on the fundamental mechanism of wound healing. Public Library of Science 2018-07-26 /pmc/articles/PMC6062108/ /pubmed/30048525 http://dx.doi.org/10.1371/journal.pone.0201418 Text en © 2018 Lee et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lee, Insu
Kim, Daegyu
Park, Ga-Lahm
Jeon, Tae-Joon
Kim, Sun Min
Investigation of wound healing process guided by nano-scale topographic patterns integrated within a microfluidic system
title Investigation of wound healing process guided by nano-scale topographic patterns integrated within a microfluidic system
title_full Investigation of wound healing process guided by nano-scale topographic patterns integrated within a microfluidic system
title_fullStr Investigation of wound healing process guided by nano-scale topographic patterns integrated within a microfluidic system
title_full_unstemmed Investigation of wound healing process guided by nano-scale topographic patterns integrated within a microfluidic system
title_short Investigation of wound healing process guided by nano-scale topographic patterns integrated within a microfluidic system
title_sort investigation of wound healing process guided by nano-scale topographic patterns integrated within a microfluidic system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062108/
https://www.ncbi.nlm.nih.gov/pubmed/30048525
http://dx.doi.org/10.1371/journal.pone.0201418
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