Cargando…

Genetic Polymorphism of CYP2A6 and Its Relationship with Nicotine Metabolism in Male Bataknese Smokers Suffered from Lung Cancer in Indonesia

BACKGROUND: Cytochrome P450 2A6 (CYP2A6) is known as an enzyme which is responsible for the metabolism of chemical compounds. AIM: This study aimed to analyse the relationship between CYP2A6 gene polymorphism with nicotine metabolism rates and lung cancer incidence among smokers of Batak ethnic grou...

Descripción completa

Detalles Bibliográficos
Autores principales: Soeroso, Noni Novisari, Zain-Hamid, Rozaimah, Sinaga, Bintang Y. M., Sadewa, Ahmad Hamim, Syafiuddin, Tamsil, Syahruddin, Elisna, Tann, Gino, Mutiara, Erna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Republic of Macedonia 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062282/
https://www.ncbi.nlm.nih.gov/pubmed/30087722
http://dx.doi.org/10.3889/oamjms.2018.259
_version_ 1783342363878359040
author Soeroso, Noni Novisari
Zain-Hamid, Rozaimah
Sinaga, Bintang Y. M.
Sadewa, Ahmad Hamim
Syafiuddin, Tamsil
Syahruddin, Elisna
Tann, Gino
Mutiara, Erna
author_facet Soeroso, Noni Novisari
Zain-Hamid, Rozaimah
Sinaga, Bintang Y. M.
Sadewa, Ahmad Hamim
Syafiuddin, Tamsil
Syahruddin, Elisna
Tann, Gino
Mutiara, Erna
author_sort Soeroso, Noni Novisari
collection PubMed
description BACKGROUND: Cytochrome P450 2A6 (CYP2A6) is known as an enzyme which is responsible for the metabolism of chemical compounds. AIM: This study aimed to analyse the relationship between CYP2A6 gene polymorphism with nicotine metabolism rates and lung cancer incidence among smokers of Batak ethnic group in Indonesia. METHODS: This study was a case-control study involving 140 research subjects through a purposive sampling technique from three hospitals in Medan, Indonesia. An examination of nicotine metabolism rates was conducted for all subjects using the 3HC/cotinine ratio parameter with LC-MS/MS technique. The examination of the CYP2A6 gene was performed with PCR-RFLP. Data were analysed with Conditional Logistic Regression test using Epi Info 7.0 software. RESULTS: The allele frequencies of CYP2A6*1A, CYP2A6*1B, and CYP2A6*4A found were 44.3%, 48.9%, and 6.8%, respectively. The *1B allele showed the highest metabolism rate. It is found that slow metabolizer individuals were 5.49 times more likely to develop lung cancer (P = 0.01, 95%CI 1.2-24.8). CONCLUSION: Among the Bataknese smokers studied, the CYP2A6*1B allele was found to be the most common allele and showed the highest rate of nicotine metabolism. However, the results show the insignificant relationship among CYP2A6 genetic polymorphism, nicotine metabolism, and lung cancer incidence.
format Online
Article
Text
id pubmed-6062282
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Republic of Macedonia
record_format MEDLINE/PubMed
spelling pubmed-60622822018-08-07 Genetic Polymorphism of CYP2A6 and Its Relationship with Nicotine Metabolism in Male Bataknese Smokers Suffered from Lung Cancer in Indonesia Soeroso, Noni Novisari Zain-Hamid, Rozaimah Sinaga, Bintang Y. M. Sadewa, Ahmad Hamim Syafiuddin, Tamsil Syahruddin, Elisna Tann, Gino Mutiara, Erna Open Access Maced J Med Sci Basic Science BACKGROUND: Cytochrome P450 2A6 (CYP2A6) is known as an enzyme which is responsible for the metabolism of chemical compounds. AIM: This study aimed to analyse the relationship between CYP2A6 gene polymorphism with nicotine metabolism rates and lung cancer incidence among smokers of Batak ethnic group in Indonesia. METHODS: This study was a case-control study involving 140 research subjects through a purposive sampling technique from three hospitals in Medan, Indonesia. An examination of nicotine metabolism rates was conducted for all subjects using the 3HC/cotinine ratio parameter with LC-MS/MS technique. The examination of the CYP2A6 gene was performed with PCR-RFLP. Data were analysed with Conditional Logistic Regression test using Epi Info 7.0 software. RESULTS: The allele frequencies of CYP2A6*1A, CYP2A6*1B, and CYP2A6*4A found were 44.3%, 48.9%, and 6.8%, respectively. The *1B allele showed the highest metabolism rate. It is found that slow metabolizer individuals were 5.49 times more likely to develop lung cancer (P = 0.01, 95%CI 1.2-24.8). CONCLUSION: Among the Bataknese smokers studied, the CYP2A6*1B allele was found to be the most common allele and showed the highest rate of nicotine metabolism. However, the results show the insignificant relationship among CYP2A6 genetic polymorphism, nicotine metabolism, and lung cancer incidence. Republic of Macedonia 2018-07-15 /pmc/articles/PMC6062282/ /pubmed/30087722 http://dx.doi.org/10.3889/oamjms.2018.259 Text en Copyright: © 2018 Noni Novisari Soeroso, Rozaimah Zain-Hamid, Bintang Y. M. Sinaga, Ahmad Hamim Sadewa, Tamsil Syafiuddin, Elisna Syahruddin, Gino Tann, Erna Mutiara http://creativecommons.org/licenses/CC BY-NC/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
spellingShingle Basic Science
Soeroso, Noni Novisari
Zain-Hamid, Rozaimah
Sinaga, Bintang Y. M.
Sadewa, Ahmad Hamim
Syafiuddin, Tamsil
Syahruddin, Elisna
Tann, Gino
Mutiara, Erna
Genetic Polymorphism of CYP2A6 and Its Relationship with Nicotine Metabolism in Male Bataknese Smokers Suffered from Lung Cancer in Indonesia
title Genetic Polymorphism of CYP2A6 and Its Relationship with Nicotine Metabolism in Male Bataknese Smokers Suffered from Lung Cancer in Indonesia
title_full Genetic Polymorphism of CYP2A6 and Its Relationship with Nicotine Metabolism in Male Bataknese Smokers Suffered from Lung Cancer in Indonesia
title_fullStr Genetic Polymorphism of CYP2A6 and Its Relationship with Nicotine Metabolism in Male Bataknese Smokers Suffered from Lung Cancer in Indonesia
title_full_unstemmed Genetic Polymorphism of CYP2A6 and Its Relationship with Nicotine Metabolism in Male Bataknese Smokers Suffered from Lung Cancer in Indonesia
title_short Genetic Polymorphism of CYP2A6 and Its Relationship with Nicotine Metabolism in Male Bataknese Smokers Suffered from Lung Cancer in Indonesia
title_sort genetic polymorphism of cyp2a6 and its relationship with nicotine metabolism in male bataknese smokers suffered from lung cancer in indonesia
topic Basic Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062282/
https://www.ncbi.nlm.nih.gov/pubmed/30087722
http://dx.doi.org/10.3889/oamjms.2018.259
work_keys_str_mv AT soerosononinovisari geneticpolymorphismofcyp2a6anditsrelationshipwithnicotinemetabolisminmalebataknesesmokerssufferedfromlungcancerinindonesia
AT zainhamidrozaimah geneticpolymorphismofcyp2a6anditsrelationshipwithnicotinemetabolisminmalebataknesesmokerssufferedfromlungcancerinindonesia
AT sinagabintangym geneticpolymorphismofcyp2a6anditsrelationshipwithnicotinemetabolisminmalebataknesesmokerssufferedfromlungcancerinindonesia
AT sadewaahmadhamim geneticpolymorphismofcyp2a6anditsrelationshipwithnicotinemetabolisminmalebataknesesmokerssufferedfromlungcancerinindonesia
AT syafiuddintamsil geneticpolymorphismofcyp2a6anditsrelationshipwithnicotinemetabolisminmalebataknesesmokerssufferedfromlungcancerinindonesia
AT syahruddinelisna geneticpolymorphismofcyp2a6anditsrelationshipwithnicotinemetabolisminmalebataknesesmokerssufferedfromlungcancerinindonesia
AT tanngino geneticpolymorphismofcyp2a6anditsrelationshipwithnicotinemetabolisminmalebataknesesmokerssufferedfromlungcancerinindonesia
AT mutiaraerna geneticpolymorphismofcyp2a6anditsrelationshipwithnicotinemetabolisminmalebataknesesmokerssufferedfromlungcancerinindonesia