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Cardiovascular progenitor cells cultured aboard the International Space Station exhibit altered developmental and functional properties
The heart and its cellular components are profoundly altered by missions to space and injury on Earth. Further research, however, is needed to characterize and address the molecular substrates of such changes. For this reason, neonatal and adult human cardiovascular progenitor cells (CPCs) were cult...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062551/ https://www.ncbi.nlm.nih.gov/pubmed/30062101 http://dx.doi.org/10.1038/s41526-018-0048-x |
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author | Baio, Jonathan Martinez, Aida F. Silva, Ivan Hoehn, Carla V. Countryman, Stephanie Bailey, Leonard Hasaniya, Nahidh Pecaut, Michael J. Kearns-Jonker, Mary |
author_facet | Baio, Jonathan Martinez, Aida F. Silva, Ivan Hoehn, Carla V. Countryman, Stephanie Bailey, Leonard Hasaniya, Nahidh Pecaut, Michael J. Kearns-Jonker, Mary |
author_sort | Baio, Jonathan |
collection | PubMed |
description | The heart and its cellular components are profoundly altered by missions to space and injury on Earth. Further research, however, is needed to characterize and address the molecular substrates of such changes. For this reason, neonatal and adult human cardiovascular progenitor cells (CPCs) were cultured aboard the International Space Station. Upon return to Earth, we measured changes in the expression of microRNAs and of genes related to mechanotransduction, cardiogenesis, cell cycling, DNA repair, and paracrine signaling. We additionally assessed endothelial-like tube formation, cell cycling, and migratory capacity of CPCs. Changes in microRNA expression were predicted to target extracellular matrix interactions and Hippo signaling in both neonatal and adult CPCs. Genes related to mechanotransduction (YAP1, RHOA) were downregulated, while the expression of cytoskeletal genes (VIM, NES, DES, LMNB2, LMNA), non-canonical Wnt ligands (WNT5A, WNT9A), and Wnt/calcium signaling molecules (PLCG1, PRKCA) was significantly elevated in neonatal CPCs. Increased mesendodermal gene expression along with decreased expression of mesodermal derivative markers (TNNT2, VWF, and RUNX2), reduced readiness to form endothelial-like tubes, and elevated expression of Bmp and Tbx genes, were observed in neonatal CPCs. Both neonatal and adult CPCs exhibited increased expression of DNA repair genes and paracrine factors, which was supported by enhanced migration. While spaceflight affects cytoskeletal organization and migration in neonatal and adult CPCs, only neonatal CPCs experienced increased expression of early developmental markers and an enhanced proliferative potential. Efforts to recapitulate the effects of spaceflight on Earth by regulating processes described herein may be a promising avenue for cardiac repair. |
format | Online Article Text |
id | pubmed-6062551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60625512018-07-30 Cardiovascular progenitor cells cultured aboard the International Space Station exhibit altered developmental and functional properties Baio, Jonathan Martinez, Aida F. Silva, Ivan Hoehn, Carla V. Countryman, Stephanie Bailey, Leonard Hasaniya, Nahidh Pecaut, Michael J. Kearns-Jonker, Mary NPJ Microgravity Article The heart and its cellular components are profoundly altered by missions to space and injury on Earth. Further research, however, is needed to characterize and address the molecular substrates of such changes. For this reason, neonatal and adult human cardiovascular progenitor cells (CPCs) were cultured aboard the International Space Station. Upon return to Earth, we measured changes in the expression of microRNAs and of genes related to mechanotransduction, cardiogenesis, cell cycling, DNA repair, and paracrine signaling. We additionally assessed endothelial-like tube formation, cell cycling, and migratory capacity of CPCs. Changes in microRNA expression were predicted to target extracellular matrix interactions and Hippo signaling in both neonatal and adult CPCs. Genes related to mechanotransduction (YAP1, RHOA) were downregulated, while the expression of cytoskeletal genes (VIM, NES, DES, LMNB2, LMNA), non-canonical Wnt ligands (WNT5A, WNT9A), and Wnt/calcium signaling molecules (PLCG1, PRKCA) was significantly elevated in neonatal CPCs. Increased mesendodermal gene expression along with decreased expression of mesodermal derivative markers (TNNT2, VWF, and RUNX2), reduced readiness to form endothelial-like tubes, and elevated expression of Bmp and Tbx genes, were observed in neonatal CPCs. Both neonatal and adult CPCs exhibited increased expression of DNA repair genes and paracrine factors, which was supported by enhanced migration. While spaceflight affects cytoskeletal organization and migration in neonatal and adult CPCs, only neonatal CPCs experienced increased expression of early developmental markers and an enhanced proliferative potential. Efforts to recapitulate the effects of spaceflight on Earth by regulating processes described herein may be a promising avenue for cardiac repair. Nature Publishing Group UK 2018-07-26 /pmc/articles/PMC6062551/ /pubmed/30062101 http://dx.doi.org/10.1038/s41526-018-0048-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Baio, Jonathan Martinez, Aida F. Silva, Ivan Hoehn, Carla V. Countryman, Stephanie Bailey, Leonard Hasaniya, Nahidh Pecaut, Michael J. Kearns-Jonker, Mary Cardiovascular progenitor cells cultured aboard the International Space Station exhibit altered developmental and functional properties |
title | Cardiovascular progenitor cells cultured aboard the International Space Station exhibit altered developmental and functional properties |
title_full | Cardiovascular progenitor cells cultured aboard the International Space Station exhibit altered developmental and functional properties |
title_fullStr | Cardiovascular progenitor cells cultured aboard the International Space Station exhibit altered developmental and functional properties |
title_full_unstemmed | Cardiovascular progenitor cells cultured aboard the International Space Station exhibit altered developmental and functional properties |
title_short | Cardiovascular progenitor cells cultured aboard the International Space Station exhibit altered developmental and functional properties |
title_sort | cardiovascular progenitor cells cultured aboard the international space station exhibit altered developmental and functional properties |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062551/ https://www.ncbi.nlm.nih.gov/pubmed/30062101 http://dx.doi.org/10.1038/s41526-018-0048-x |
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