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CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates

Polyploidization occurs widely in eukaryotes, and especially in plants. Polyploid plants and some fishes have been commercialized. Typically, severe genomic perturbations immediately follow polyploidization and little is known about how polyploid offspring survives the genetic and epigenetic changes...

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Autores principales: Yin, Fanqian, Liu, Wenfu, Chai, Jing, Lu, Bin, Murphy, Robert W., Luo, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062590/
https://www.ncbi.nlm.nih.gov/pubmed/30079079
http://dx.doi.org/10.3389/fgene.2018.00260
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author Yin, Fanqian
Liu, Wenfu
Chai, Jing
Lu, Bin
Murphy, Robert W.
Luo, Jing
author_facet Yin, Fanqian
Liu, Wenfu
Chai, Jing
Lu, Bin
Murphy, Robert W.
Luo, Jing
author_sort Yin, Fanqian
collection PubMed
description Polyploidization occurs widely in eukaryotes, and especially in plants. Polyploid plants and some fishes have been commercialized. Typically, severe genomic perturbations immediately follow polyploidization and little is known about how polyploid offspring survives the genetic and epigenetic changes. Investigations into this require the identification of genes related to polyploidization and the discrimination of dosage-balance from paternal and maternal copies, and regardless of the mechanism being either autopolyploidization or allopolyploidization. New approaches and technologies may discern the mosaic of novel gene functions gained through the recombination of paternal and maternal genes in allopolyploidization. Modifications of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) with CRISPR-associated system (Cas) protein 9 (CRISPR/Cas9) have been employed in studies of polyploidization of plants. However, the approach has seldom been applied to polyploidization in vertebrates. Herein, we use CRISPR/Cas9 to trace gene-fate in tetraploid goldfish, and specifically to identify the functional differentiation of two divergent copies of fgf20a, which are expressed differently throughout embryonic development. We expect this gene editing system will be applicable to studies of polyploids and the genetic improvement of polyploid livestock.
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spelling pubmed-60625902018-08-03 CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates Yin, Fanqian Liu, Wenfu Chai, Jing Lu, Bin Murphy, Robert W. Luo, Jing Front Genet Genetics Polyploidization occurs widely in eukaryotes, and especially in plants. Polyploid plants and some fishes have been commercialized. Typically, severe genomic perturbations immediately follow polyploidization and little is known about how polyploid offspring survives the genetic and epigenetic changes. Investigations into this require the identification of genes related to polyploidization and the discrimination of dosage-balance from paternal and maternal copies, and regardless of the mechanism being either autopolyploidization or allopolyploidization. New approaches and technologies may discern the mosaic of novel gene functions gained through the recombination of paternal and maternal genes in allopolyploidization. Modifications of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) with CRISPR-associated system (Cas) protein 9 (CRISPR/Cas9) have been employed in studies of polyploidization of plants. However, the approach has seldom been applied to polyploidization in vertebrates. Herein, we use CRISPR/Cas9 to trace gene-fate in tetraploid goldfish, and specifically to identify the functional differentiation of two divergent copies of fgf20a, which are expressed differently throughout embryonic development. We expect this gene editing system will be applicable to studies of polyploids and the genetic improvement of polyploid livestock. Frontiers Media S.A. 2018-07-20 /pmc/articles/PMC6062590/ /pubmed/30079079 http://dx.doi.org/10.3389/fgene.2018.00260 Text en Copyright © 2018 Yin, Liu, Chai, Lu, Murphy and Luo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Yin, Fanqian
Liu, Wenfu
Chai, Jing
Lu, Bin
Murphy, Robert W.
Luo, Jing
CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates
title CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates
title_full CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates
title_fullStr CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates
title_full_unstemmed CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates
title_short CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates
title_sort crispr/cas9 application for gene copy fate survey of polyploid vertebrates
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062590/
https://www.ncbi.nlm.nih.gov/pubmed/30079079
http://dx.doi.org/10.3389/fgene.2018.00260
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