Cargando…
A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties
Streptococcus pneumoniae, a Gram-positive human pathogen, has evolved three main transporters for iron acquisition from the host: PiaABC, PiuABC, and PitABC. Our previous study had shown that the mRNA and protein levels of SPD_1590 are significantly upregulated in the ΔpiuA/ΔpiaA/ΔpitA triple mutant...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062600/ https://www.ncbi.nlm.nih.gov/pubmed/30079056 http://dx.doi.org/10.3389/fmicb.2018.01624 |
_version_ | 1783342404298866688 |
---|---|
author | Miao, Xinyu He, Jiaojiao Zhang, Liang Zhao, Xinlu Ge, Ruiguang He, Qing-Yu Sun, Xuesong |
author_facet | Miao, Xinyu He, Jiaojiao Zhang, Liang Zhao, Xinlu Ge, Ruiguang He, Qing-Yu Sun, Xuesong |
author_sort | Miao, Xinyu |
collection | PubMed |
description | Streptococcus pneumoniae, a Gram-positive human pathogen, has evolved three main transporters for iron acquisition from the host: PiaABC, PiuABC, and PitABC. Our previous study had shown that the mRNA and protein levels of SPD_1590 are significantly upregulated in the ΔpiuA/ΔpiaA/ΔpitA triple mutant, suggesting that SPD_1590 might be a novel iron transporter in S. pneumoniae. In the present study, using spd1590-knockout, -complemented, and -overexpressing strains and the purified SPD_1590 protein, we show that SPD_1590 can bind hemin, probably supplementing the function of PiuABC, to provide the iron necessary for the bacterium. Furthermore, the results of iTRAQ quantitative proteomics and cell-infection studies demonstrate that, similarly to other metal-ion uptake proteins, SPD_1590 is important for bacterial virulence properties. Overall, these results provide a better understanding of the biology of this clinically important bacterium. |
format | Online Article Text |
id | pubmed-6062600 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60626002018-08-03 A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties Miao, Xinyu He, Jiaojiao Zhang, Liang Zhao, Xinlu Ge, Ruiguang He, Qing-Yu Sun, Xuesong Front Microbiol Microbiology Streptococcus pneumoniae, a Gram-positive human pathogen, has evolved three main transporters for iron acquisition from the host: PiaABC, PiuABC, and PitABC. Our previous study had shown that the mRNA and protein levels of SPD_1590 are significantly upregulated in the ΔpiuA/ΔpiaA/ΔpitA triple mutant, suggesting that SPD_1590 might be a novel iron transporter in S. pneumoniae. In the present study, using spd1590-knockout, -complemented, and -overexpressing strains and the purified SPD_1590 protein, we show that SPD_1590 can bind hemin, probably supplementing the function of PiuABC, to provide the iron necessary for the bacterium. Furthermore, the results of iTRAQ quantitative proteomics and cell-infection studies demonstrate that, similarly to other metal-ion uptake proteins, SPD_1590 is important for bacterial virulence properties. Overall, these results provide a better understanding of the biology of this clinically important bacterium. Frontiers Media S.A. 2018-07-20 /pmc/articles/PMC6062600/ /pubmed/30079056 http://dx.doi.org/10.3389/fmicb.2018.01624 Text en Copyright © 2018 Miao, He, Zhang, Zhao, Ge, He and Sun. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Miao, Xinyu He, Jiaojiao Zhang, Liang Zhao, Xinlu Ge, Ruiguang He, Qing-Yu Sun, Xuesong A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties |
title | A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties |
title_full | A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties |
title_fullStr | A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties |
title_full_unstemmed | A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties |
title_short | A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties |
title_sort | novel iron transporter spd_1590 in streptococcus pneumoniae contributing to bacterial virulence properties |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062600/ https://www.ncbi.nlm.nih.gov/pubmed/30079056 http://dx.doi.org/10.3389/fmicb.2018.01624 |
work_keys_str_mv | AT miaoxinyu anovelirontransporterspd1590instreptococcuspneumoniaecontributingtobacterialvirulenceproperties AT hejiaojiao anovelirontransporterspd1590instreptococcuspneumoniaecontributingtobacterialvirulenceproperties AT zhangliang anovelirontransporterspd1590instreptococcuspneumoniaecontributingtobacterialvirulenceproperties AT zhaoxinlu anovelirontransporterspd1590instreptococcuspneumoniaecontributingtobacterialvirulenceproperties AT geruiguang anovelirontransporterspd1590instreptococcuspneumoniaecontributingtobacterialvirulenceproperties AT heqingyu anovelirontransporterspd1590instreptococcuspneumoniaecontributingtobacterialvirulenceproperties AT sunxuesong anovelirontransporterspd1590instreptococcuspneumoniaecontributingtobacterialvirulenceproperties AT miaoxinyu novelirontransporterspd1590instreptococcuspneumoniaecontributingtobacterialvirulenceproperties AT hejiaojiao novelirontransporterspd1590instreptococcuspneumoniaecontributingtobacterialvirulenceproperties AT zhangliang novelirontransporterspd1590instreptococcuspneumoniaecontributingtobacterialvirulenceproperties AT zhaoxinlu novelirontransporterspd1590instreptococcuspneumoniaecontributingtobacterialvirulenceproperties AT geruiguang novelirontransporterspd1590instreptococcuspneumoniaecontributingtobacterialvirulenceproperties AT heqingyu novelirontransporterspd1590instreptococcuspneumoniaecontributingtobacterialvirulenceproperties AT sunxuesong novelirontransporterspd1590instreptococcuspneumoniaecontributingtobacterialvirulenceproperties |