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A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties

Streptococcus pneumoniae, a Gram-positive human pathogen, has evolved three main transporters for iron acquisition from the host: PiaABC, PiuABC, and PitABC. Our previous study had shown that the mRNA and protein levels of SPD_1590 are significantly upregulated in the ΔpiuA/ΔpiaA/ΔpitA triple mutant...

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Autores principales: Miao, Xinyu, He, Jiaojiao, Zhang, Liang, Zhao, Xinlu, Ge, Ruiguang, He, Qing-Yu, Sun, Xuesong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062600/
https://www.ncbi.nlm.nih.gov/pubmed/30079056
http://dx.doi.org/10.3389/fmicb.2018.01624
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author Miao, Xinyu
He, Jiaojiao
Zhang, Liang
Zhao, Xinlu
Ge, Ruiguang
He, Qing-Yu
Sun, Xuesong
author_facet Miao, Xinyu
He, Jiaojiao
Zhang, Liang
Zhao, Xinlu
Ge, Ruiguang
He, Qing-Yu
Sun, Xuesong
author_sort Miao, Xinyu
collection PubMed
description Streptococcus pneumoniae, a Gram-positive human pathogen, has evolved three main transporters for iron acquisition from the host: PiaABC, PiuABC, and PitABC. Our previous study had shown that the mRNA and protein levels of SPD_1590 are significantly upregulated in the ΔpiuA/ΔpiaA/ΔpitA triple mutant, suggesting that SPD_1590 might be a novel iron transporter in S. pneumoniae. In the present study, using spd1590-knockout, -complemented, and -overexpressing strains and the purified SPD_1590 protein, we show that SPD_1590 can bind hemin, probably supplementing the function of PiuABC, to provide the iron necessary for the bacterium. Furthermore, the results of iTRAQ quantitative proteomics and cell-infection studies demonstrate that, similarly to other metal-ion uptake proteins, SPD_1590 is important for bacterial virulence properties. Overall, these results provide a better understanding of the biology of this clinically important bacterium.
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spelling pubmed-60626002018-08-03 A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties Miao, Xinyu He, Jiaojiao Zhang, Liang Zhao, Xinlu Ge, Ruiguang He, Qing-Yu Sun, Xuesong Front Microbiol Microbiology Streptococcus pneumoniae, a Gram-positive human pathogen, has evolved three main transporters for iron acquisition from the host: PiaABC, PiuABC, and PitABC. Our previous study had shown that the mRNA and protein levels of SPD_1590 are significantly upregulated in the ΔpiuA/ΔpiaA/ΔpitA triple mutant, suggesting that SPD_1590 might be a novel iron transporter in S. pneumoniae. In the present study, using spd1590-knockout, -complemented, and -overexpressing strains and the purified SPD_1590 protein, we show that SPD_1590 can bind hemin, probably supplementing the function of PiuABC, to provide the iron necessary for the bacterium. Furthermore, the results of iTRAQ quantitative proteomics and cell-infection studies demonstrate that, similarly to other metal-ion uptake proteins, SPD_1590 is important for bacterial virulence properties. Overall, these results provide a better understanding of the biology of this clinically important bacterium. Frontiers Media S.A. 2018-07-20 /pmc/articles/PMC6062600/ /pubmed/30079056 http://dx.doi.org/10.3389/fmicb.2018.01624 Text en Copyright © 2018 Miao, He, Zhang, Zhao, Ge, He and Sun. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Miao, Xinyu
He, Jiaojiao
Zhang, Liang
Zhao, Xinlu
Ge, Ruiguang
He, Qing-Yu
Sun, Xuesong
A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties
title A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties
title_full A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties
title_fullStr A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties
title_full_unstemmed A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties
title_short A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties
title_sort novel iron transporter spd_1590 in streptococcus pneumoniae contributing to bacterial virulence properties
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062600/
https://www.ncbi.nlm.nih.gov/pubmed/30079056
http://dx.doi.org/10.3389/fmicb.2018.01624
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