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Inverted signaling by bacterial chemotaxis receptors

Microorganisms use transmembrane sensory receptors to perceive a wide range of environmental factors. It is unclear how rapidly the sensory properties of these receptors can be modified when microorganisms adapt to novel environments. Here, we demonstrate experimentally that the response of an Esche...

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Detalles Bibliográficos
Autores principales: Bi, Shuangyu, Jin, Fan, Sourjik, Victor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062612/
https://www.ncbi.nlm.nih.gov/pubmed/30050034
http://dx.doi.org/10.1038/s41467-018-05335-w
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author Bi, Shuangyu
Jin, Fan
Sourjik, Victor
author_facet Bi, Shuangyu
Jin, Fan
Sourjik, Victor
author_sort Bi, Shuangyu
collection PubMed
description Microorganisms use transmembrane sensory receptors to perceive a wide range of environmental factors. It is unclear how rapidly the sensory properties of these receptors can be modified when microorganisms adapt to novel environments. Here, we demonstrate experimentally that the response of an Escherichia coli chemotaxis receptor to its chemical ligands can be easily inverted by mutations at several sites along receptor sequence. We also perform molecular dynamics simulations to shed light on the mechanism of the transmembrane signaling by E. coli chemoreceptors. Finally, we use receptors with inverted signaling to map determinants that enable the same receptor to sense multiple environmental factors, including metal ions, aromatic compounds, osmotic pressure, and salt ions. Our findings demonstrate high plasticity of signaling and provide further insights into the mechanisms of stimulus sensing and processing by bacterial chemoreceptors.
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spelling pubmed-60626122018-07-30 Inverted signaling by bacterial chemotaxis receptors Bi, Shuangyu Jin, Fan Sourjik, Victor Nat Commun Article Microorganisms use transmembrane sensory receptors to perceive a wide range of environmental factors. It is unclear how rapidly the sensory properties of these receptors can be modified when microorganisms adapt to novel environments. Here, we demonstrate experimentally that the response of an Escherichia coli chemotaxis receptor to its chemical ligands can be easily inverted by mutations at several sites along receptor sequence. We also perform molecular dynamics simulations to shed light on the mechanism of the transmembrane signaling by E. coli chemoreceptors. Finally, we use receptors with inverted signaling to map determinants that enable the same receptor to sense multiple environmental factors, including metal ions, aromatic compounds, osmotic pressure, and salt ions. Our findings demonstrate high plasticity of signaling and provide further insights into the mechanisms of stimulus sensing and processing by bacterial chemoreceptors. Nature Publishing Group UK 2018-07-26 /pmc/articles/PMC6062612/ /pubmed/30050034 http://dx.doi.org/10.1038/s41467-018-05335-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bi, Shuangyu
Jin, Fan
Sourjik, Victor
Inverted signaling by bacterial chemotaxis receptors
title Inverted signaling by bacterial chemotaxis receptors
title_full Inverted signaling by bacterial chemotaxis receptors
title_fullStr Inverted signaling by bacterial chemotaxis receptors
title_full_unstemmed Inverted signaling by bacterial chemotaxis receptors
title_short Inverted signaling by bacterial chemotaxis receptors
title_sort inverted signaling by bacterial chemotaxis receptors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062612/
https://www.ncbi.nlm.nih.gov/pubmed/30050034
http://dx.doi.org/10.1038/s41467-018-05335-w
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