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A Rapid Immunochromatography Test Based on Hcp1 Is a Potential Point-of-Care Test for Serological Diagnosis of Melioidosis

Melioidosis is a fatal infectious disease caused by the environmental bacterium Burkholderia pseudomallei. It is highly endemic in Asia and northern Australia but neglected in many other tropical countries. Melioidosis patients have a wide range of clinical manifestations, and definitive diagnosis r...

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Autores principales: Phokrai, Phornpun, Karoonboonyanan, Wisansanee, Thanapattarapairoj, Nida, Promkong, Chidchanok, Dulsuk, Adul, Koosakulnirand, Sirikamon, Canovali, Sasha, Indrawattana, Nitaya, Jutrakul, Yaowaruk, Wuthiekanun, Vanaporn, Limmathurotsakul, Direk, Brett, Paul J., Burtnick, Mary N., Lertmemongkolchai, Ganjana, Chantratita, Narisara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062804/
https://www.ncbi.nlm.nih.gov/pubmed/29848565
http://dx.doi.org/10.1128/JCM.00346-18
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author Phokrai, Phornpun
Karoonboonyanan, Wisansanee
Thanapattarapairoj, Nida
Promkong, Chidchanok
Dulsuk, Adul
Koosakulnirand, Sirikamon
Canovali, Sasha
Indrawattana, Nitaya
Jutrakul, Yaowaruk
Wuthiekanun, Vanaporn
Limmathurotsakul, Direk
Brett, Paul J.
Burtnick, Mary N.
Lertmemongkolchai, Ganjana
Chantratita, Narisara
author_facet Phokrai, Phornpun
Karoonboonyanan, Wisansanee
Thanapattarapairoj, Nida
Promkong, Chidchanok
Dulsuk, Adul
Koosakulnirand, Sirikamon
Canovali, Sasha
Indrawattana, Nitaya
Jutrakul, Yaowaruk
Wuthiekanun, Vanaporn
Limmathurotsakul, Direk
Brett, Paul J.
Burtnick, Mary N.
Lertmemongkolchai, Ganjana
Chantratita, Narisara
author_sort Phokrai, Phornpun
collection PubMed
description Melioidosis is a fatal infectious disease caused by the environmental bacterium Burkholderia pseudomallei. It is highly endemic in Asia and northern Australia but neglected in many other tropical countries. Melioidosis patients have a wide range of clinical manifestations, and definitive diagnosis requires bacterial culture, which can be time-consuming. A reliable rapid serological tool is greatly needed for disease surveillance and diagnosis. We previously demonstrated by enzyme-linked immunosorbent assay (ELISA) that a hemolysin-coregulated protein (Hcp1) is a promising target for serodiagnosis of melioidosis. In this study, we developed a rapid immunochromatography test (ICT) using Hcp1 as the target antigen (Hcp1-ICT). We evaluated this test for specific antibody detection using serum samples obtained from 4 groups of human subjects, including the following: (i) 487 culture-confirmed melioidosis patients from four hospitals in northeast Thailand; (ii) 202 healthy donors from northeast Thailand; (iii) 90 U.S. healthy donors; and (iv) 207 patients infected with other organisms. Compared to culture results as a gold standard, the sensitivity of ICT for all hospitals was 88.3%. The specificities for Thai donors and U.S. donors were 86.1% and 100%, respectively, and the specificity for other infections was 91.8%. The results of the Hcp1-ICT demonstrated 92.4% agreement with the Hcp1-ELISA results with a kappa value of 0.829, indicating that the method is much improved compared with the current serological method, the indirect hemagglutination assay (IHA) (69.5% sensitivity and 67.6% specificity for Thais). The Hcp1-ICT represents a potential point-of-care (POC) test and may be used to replace the IHA for screening of melioidosis in hospitals as well as in resource-limited areas.
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spelling pubmed-60628042018-08-08 A Rapid Immunochromatography Test Based on Hcp1 Is a Potential Point-of-Care Test for Serological Diagnosis of Melioidosis Phokrai, Phornpun Karoonboonyanan, Wisansanee Thanapattarapairoj, Nida Promkong, Chidchanok Dulsuk, Adul Koosakulnirand, Sirikamon Canovali, Sasha Indrawattana, Nitaya Jutrakul, Yaowaruk Wuthiekanun, Vanaporn Limmathurotsakul, Direk Brett, Paul J. Burtnick, Mary N. Lertmemongkolchai, Ganjana Chantratita, Narisara J Clin Microbiol Immunoassays Melioidosis is a fatal infectious disease caused by the environmental bacterium Burkholderia pseudomallei. It is highly endemic in Asia and northern Australia but neglected in many other tropical countries. Melioidosis patients have a wide range of clinical manifestations, and definitive diagnosis requires bacterial culture, which can be time-consuming. A reliable rapid serological tool is greatly needed for disease surveillance and diagnosis. We previously demonstrated by enzyme-linked immunosorbent assay (ELISA) that a hemolysin-coregulated protein (Hcp1) is a promising target for serodiagnosis of melioidosis. In this study, we developed a rapid immunochromatography test (ICT) using Hcp1 as the target antigen (Hcp1-ICT). We evaluated this test for specific antibody detection using serum samples obtained from 4 groups of human subjects, including the following: (i) 487 culture-confirmed melioidosis patients from four hospitals in northeast Thailand; (ii) 202 healthy donors from northeast Thailand; (iii) 90 U.S. healthy donors; and (iv) 207 patients infected with other organisms. Compared to culture results as a gold standard, the sensitivity of ICT for all hospitals was 88.3%. The specificities for Thai donors and U.S. donors were 86.1% and 100%, respectively, and the specificity for other infections was 91.8%. The results of the Hcp1-ICT demonstrated 92.4% agreement with the Hcp1-ELISA results with a kappa value of 0.829, indicating that the method is much improved compared with the current serological method, the indirect hemagglutination assay (IHA) (69.5% sensitivity and 67.6% specificity for Thais). The Hcp1-ICT represents a potential point-of-care (POC) test and may be used to replace the IHA for screening of melioidosis in hospitals as well as in resource-limited areas. American Society for Microbiology 2018-07-26 /pmc/articles/PMC6062804/ /pubmed/29848565 http://dx.doi.org/10.1128/JCM.00346-18 Text en Copyright © 2018 Phokrai et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Immunoassays
Phokrai, Phornpun
Karoonboonyanan, Wisansanee
Thanapattarapairoj, Nida
Promkong, Chidchanok
Dulsuk, Adul
Koosakulnirand, Sirikamon
Canovali, Sasha
Indrawattana, Nitaya
Jutrakul, Yaowaruk
Wuthiekanun, Vanaporn
Limmathurotsakul, Direk
Brett, Paul J.
Burtnick, Mary N.
Lertmemongkolchai, Ganjana
Chantratita, Narisara
A Rapid Immunochromatography Test Based on Hcp1 Is a Potential Point-of-Care Test for Serological Diagnosis of Melioidosis
title A Rapid Immunochromatography Test Based on Hcp1 Is a Potential Point-of-Care Test for Serological Diagnosis of Melioidosis
title_full A Rapid Immunochromatography Test Based on Hcp1 Is a Potential Point-of-Care Test for Serological Diagnosis of Melioidosis
title_fullStr A Rapid Immunochromatography Test Based on Hcp1 Is a Potential Point-of-Care Test for Serological Diagnosis of Melioidosis
title_full_unstemmed A Rapid Immunochromatography Test Based on Hcp1 Is a Potential Point-of-Care Test for Serological Diagnosis of Melioidosis
title_short A Rapid Immunochromatography Test Based on Hcp1 Is a Potential Point-of-Care Test for Serological Diagnosis of Melioidosis
title_sort rapid immunochromatography test based on hcp1 is a potential point-of-care test for serological diagnosis of melioidosis
topic Immunoassays
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062804/
https://www.ncbi.nlm.nih.gov/pubmed/29848565
http://dx.doi.org/10.1128/JCM.00346-18
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