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The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco
BACKGROUND: Hypertension is a multifactorial disease involving both environmental and genetic Factros. G894 T eNOS polymorphism has been suggested to be responsible for reduced NO synthesis, and EH development. The objective of our case-control study is to evaluate the potential association of G894 ...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062870/ https://www.ncbi.nlm.nih.gov/pubmed/30053839 http://dx.doi.org/10.1186/s12881-018-0638-1 |
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author | Nassereddine, Sanaa Hassani Idrissi, Hind Habbal, Rachida Abouelfath, Rhizlane Korch, Farah Haraka, Majda Karkar, Adnane Nadifi, Sellama |
author_facet | Nassereddine, Sanaa Hassani Idrissi, Hind Habbal, Rachida Abouelfath, Rhizlane Korch, Farah Haraka, Majda Karkar, Adnane Nadifi, Sellama |
author_sort | Nassereddine, Sanaa |
collection | PubMed |
description | BACKGROUND: Hypertension is a multifactorial disease involving both environmental and genetic Factros. G894 T eNOS polymorphism has been suggested to be responsible for reduced NO synthesis, and EH development. The objective of our case-control study is to evaluate the potential association of G894 T eNOS polymorphism with Essential Hypertension (EH) susceptibility, among a sample of Moroccan patients. METHODS: One hundred forty five hypertensive patients were recruited from the department of Cardiology, University Hospital Center Ibn Rochd, Casablanca, Morocco, and compared to 184 apparently healthy subjects. DNA samples were genotype by PCR-RFLP method using MboI restriction enzyme. RESULTS: Our results showed a positive correlation between G894 T eNOS distribution and Alcohol and Obesity rik factors (P = 0.009 and 0.02 respectively). Patients with elevated Cardio Vascular Risk (CVR) carried out the higher frequency of homozygous mutant genotype TT (62.2%) and T mutant allele (77.8%), compared to median and low CVR groups. G894 T eNOS distribution was significantly associated to a high risk of EH occurrence under the GT and TT genotypes (OR [95% CI] = 20.2 [7.7–52.4], P < 0.0001; OR [95% CI] = 332.5 [98.2–1125.4], P < 0.0001 respectively), and the 3 genotypic transmission models (Dominant: OR [95% CI] = 43.2 [17.9–104.09], P < 0.0001; Recessive: OR [95% CI] = 47.7 [18.6–122.3]; P < 0.0001; Additive: OR [95% CI] = 14.02 [9.6–20.45], P < 0.0001). CONCLUSION: Our study suggests a strong association of G894 T eNOS polymorphism with susceptibility to EH in Morocco. Studies trying to identify contributing genes may be very useful and allow recognizing the vulnerable individuals and classifying patients in subgroups with definite genetic and pathogenic mechanisms to achieve better prevention and therapeutics. |
format | Online Article Text |
id | pubmed-6062870 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-60628702018-07-31 The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco Nassereddine, Sanaa Hassani Idrissi, Hind Habbal, Rachida Abouelfath, Rhizlane Korch, Farah Haraka, Majda Karkar, Adnane Nadifi, Sellama BMC Med Genet Research Article BACKGROUND: Hypertension is a multifactorial disease involving both environmental and genetic Factros. G894 T eNOS polymorphism has been suggested to be responsible for reduced NO synthesis, and EH development. The objective of our case-control study is to evaluate the potential association of G894 T eNOS polymorphism with Essential Hypertension (EH) susceptibility, among a sample of Moroccan patients. METHODS: One hundred forty five hypertensive patients were recruited from the department of Cardiology, University Hospital Center Ibn Rochd, Casablanca, Morocco, and compared to 184 apparently healthy subjects. DNA samples were genotype by PCR-RFLP method using MboI restriction enzyme. RESULTS: Our results showed a positive correlation between G894 T eNOS distribution and Alcohol and Obesity rik factors (P = 0.009 and 0.02 respectively). Patients with elevated Cardio Vascular Risk (CVR) carried out the higher frequency of homozygous mutant genotype TT (62.2%) and T mutant allele (77.8%), compared to median and low CVR groups. G894 T eNOS distribution was significantly associated to a high risk of EH occurrence under the GT and TT genotypes (OR [95% CI] = 20.2 [7.7–52.4], P < 0.0001; OR [95% CI] = 332.5 [98.2–1125.4], P < 0.0001 respectively), and the 3 genotypic transmission models (Dominant: OR [95% CI] = 43.2 [17.9–104.09], P < 0.0001; Recessive: OR [95% CI] = 47.7 [18.6–122.3]; P < 0.0001; Additive: OR [95% CI] = 14.02 [9.6–20.45], P < 0.0001). CONCLUSION: Our study suggests a strong association of G894 T eNOS polymorphism with susceptibility to EH in Morocco. Studies trying to identify contributing genes may be very useful and allow recognizing the vulnerable individuals and classifying patients in subgroups with definite genetic and pathogenic mechanisms to achieve better prevention and therapeutics. BioMed Central 2018-07-27 /pmc/articles/PMC6062870/ /pubmed/30053839 http://dx.doi.org/10.1186/s12881-018-0638-1 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Nassereddine, Sanaa Hassani Idrissi, Hind Habbal, Rachida Abouelfath, Rhizlane Korch, Farah Haraka, Majda Karkar, Adnane Nadifi, Sellama The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco |
title | The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco |
title_full | The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco |
title_fullStr | The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco |
title_full_unstemmed | The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco |
title_short | The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco |
title_sort | polymorphism g894 t of endothelial nitric oxide synthase (enos) gene is associated with susceptibility to essential hypertension (eh) in morocco |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062870/ https://www.ncbi.nlm.nih.gov/pubmed/30053839 http://dx.doi.org/10.1186/s12881-018-0638-1 |
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