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The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco

BACKGROUND: Hypertension is a multifactorial disease involving both environmental and genetic Factros. G894 T eNOS polymorphism has been suggested to be responsible for reduced NO synthesis, and EH development. The objective of our case-control study is to evaluate the potential association of G894 ...

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Autores principales: Nassereddine, Sanaa, Hassani Idrissi, Hind, Habbal, Rachida, Abouelfath, Rhizlane, Korch, Farah, Haraka, Majda, Karkar, Adnane, Nadifi, Sellama
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062870/
https://www.ncbi.nlm.nih.gov/pubmed/30053839
http://dx.doi.org/10.1186/s12881-018-0638-1
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author Nassereddine, Sanaa
Hassani Idrissi, Hind
Habbal, Rachida
Abouelfath, Rhizlane
Korch, Farah
Haraka, Majda
Karkar, Adnane
Nadifi, Sellama
author_facet Nassereddine, Sanaa
Hassani Idrissi, Hind
Habbal, Rachida
Abouelfath, Rhizlane
Korch, Farah
Haraka, Majda
Karkar, Adnane
Nadifi, Sellama
author_sort Nassereddine, Sanaa
collection PubMed
description BACKGROUND: Hypertension is a multifactorial disease involving both environmental and genetic Factros. G894 T eNOS polymorphism has been suggested to be responsible for reduced NO synthesis, and EH development. The objective of our case-control study is to evaluate the potential association of G894 T eNOS polymorphism with Essential Hypertension (EH) susceptibility, among a sample of Moroccan patients. METHODS: One hundred forty five hypertensive patients were recruited from the department of Cardiology, University Hospital Center Ibn Rochd, Casablanca, Morocco, and compared to 184 apparently healthy subjects. DNA samples were genotype by PCR-RFLP method using MboI restriction enzyme. RESULTS: Our results showed a positive correlation between G894 T eNOS distribution and Alcohol and Obesity rik factors (P = 0.009 and 0.02 respectively). Patients with elevated Cardio Vascular Risk (CVR) carried out the higher frequency of homozygous mutant genotype TT (62.2%) and T mutant allele (77.8%), compared to median and low CVR groups. G894 T eNOS distribution was significantly associated to a high risk of EH occurrence under the GT and TT genotypes (OR [95% CI] = 20.2 [7.7–52.4], P <  0.0001; OR [95% CI] = 332.5 [98.2–1125.4], P <  0.0001 respectively), and the 3 genotypic transmission models (Dominant: OR [95% CI] = 43.2 [17.9–104.09], P <  0.0001; Recessive: OR [95% CI] = 47.7 [18.6–122.3]; P <  0.0001; Additive: OR [95% CI] = 14.02 [9.6–20.45], P <  0.0001). CONCLUSION: Our study suggests a strong association of G894 T eNOS polymorphism with susceptibility to EH in Morocco. Studies trying to identify contributing genes may be very useful and allow recognizing the vulnerable individuals and classifying patients in subgroups with definite genetic and pathogenic mechanisms to achieve better prevention and therapeutics.
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spelling pubmed-60628702018-07-31 The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco Nassereddine, Sanaa Hassani Idrissi, Hind Habbal, Rachida Abouelfath, Rhizlane Korch, Farah Haraka, Majda Karkar, Adnane Nadifi, Sellama BMC Med Genet Research Article BACKGROUND: Hypertension is a multifactorial disease involving both environmental and genetic Factros. G894 T eNOS polymorphism has been suggested to be responsible for reduced NO synthesis, and EH development. The objective of our case-control study is to evaluate the potential association of G894 T eNOS polymorphism with Essential Hypertension (EH) susceptibility, among a sample of Moroccan patients. METHODS: One hundred forty five hypertensive patients were recruited from the department of Cardiology, University Hospital Center Ibn Rochd, Casablanca, Morocco, and compared to 184 apparently healthy subjects. DNA samples were genotype by PCR-RFLP method using MboI restriction enzyme. RESULTS: Our results showed a positive correlation between G894 T eNOS distribution and Alcohol and Obesity rik factors (P = 0.009 and 0.02 respectively). Patients with elevated Cardio Vascular Risk (CVR) carried out the higher frequency of homozygous mutant genotype TT (62.2%) and T mutant allele (77.8%), compared to median and low CVR groups. G894 T eNOS distribution was significantly associated to a high risk of EH occurrence under the GT and TT genotypes (OR [95% CI] = 20.2 [7.7–52.4], P <  0.0001; OR [95% CI] = 332.5 [98.2–1125.4], P <  0.0001 respectively), and the 3 genotypic transmission models (Dominant: OR [95% CI] = 43.2 [17.9–104.09], P <  0.0001; Recessive: OR [95% CI] = 47.7 [18.6–122.3]; P <  0.0001; Additive: OR [95% CI] = 14.02 [9.6–20.45], P <  0.0001). CONCLUSION: Our study suggests a strong association of G894 T eNOS polymorphism with susceptibility to EH in Morocco. Studies trying to identify contributing genes may be very useful and allow recognizing the vulnerable individuals and classifying patients in subgroups with definite genetic and pathogenic mechanisms to achieve better prevention and therapeutics. BioMed Central 2018-07-27 /pmc/articles/PMC6062870/ /pubmed/30053839 http://dx.doi.org/10.1186/s12881-018-0638-1 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Nassereddine, Sanaa
Hassani Idrissi, Hind
Habbal, Rachida
Abouelfath, Rhizlane
Korch, Farah
Haraka, Majda
Karkar, Adnane
Nadifi, Sellama
The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco
title The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco
title_full The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco
title_fullStr The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco
title_full_unstemmed The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco
title_short The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco
title_sort polymorphism g894 t of endothelial nitric oxide synthase (enos) gene is associated with susceptibility to essential hypertension (eh) in morocco
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062870/
https://www.ncbi.nlm.nih.gov/pubmed/30053839
http://dx.doi.org/10.1186/s12881-018-0638-1
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