Experimental design for single-cell RNA sequencing

Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular a...

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Detalles Bibliográficos
Autores principales: Baran-Gale, Jeanette, Chandra, Tamir, Kirschner, Kristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063265/
https://www.ncbi.nlm.nih.gov/pubmed/29126257
http://dx.doi.org/10.1093/bfgp/elx035
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author Baran-Gale, Jeanette
Chandra, Tamir
Kirschner, Kristina
author_facet Baran-Gale, Jeanette
Chandra, Tamir
Kirschner, Kristina
author_sort Baran-Gale, Jeanette
collection PubMed
description Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet-based scRNA-seq at the example of 10x Chromium. We discuss advantages and disadvantages of both methods and point out major factors to consider in designing successful experiments.
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spelling pubmed-60632652018-08-08 Experimental design for single-cell RNA sequencing Baran-Gale, Jeanette Chandra, Tamir Kirschner, Kristina Brief Funct Genomics Papers Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet-based scRNA-seq at the example of 10x Chromium. We discuss advantages and disadvantages of both methods and point out major factors to consider in designing successful experiments. Oxford University Press 2017-11-08 /pmc/articles/PMC6063265/ /pubmed/29126257 http://dx.doi.org/10.1093/bfgp/elx035 Text en © The Author 2017. Published by Oxford University Press. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Papers
Baran-Gale, Jeanette
Chandra, Tamir
Kirschner, Kristina
Experimental design for single-cell RNA sequencing
title Experimental design for single-cell RNA sequencing
title_full Experimental design for single-cell RNA sequencing
title_fullStr Experimental design for single-cell RNA sequencing
title_full_unstemmed Experimental design for single-cell RNA sequencing
title_short Experimental design for single-cell RNA sequencing
title_sort experimental design for single-cell rna sequencing
topic Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063265/
https://www.ncbi.nlm.nih.gov/pubmed/29126257
http://dx.doi.org/10.1093/bfgp/elx035
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