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Evolution of Genome Architecture in Archaea: Spontaneous Generation of a New Chromosome in Haloferax volcanii
The common ancestry of archaea and eukaryotes is evident in their genome architecture. All eukaryotic and several archaeal genomes consist of multiple chromosomes, each replicated from multiple origins. Three scenarios have been proposed for the evolution of this genome architecture: 1) mutational d...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063281/ https://www.ncbi.nlm.nih.gov/pubmed/29668953 http://dx.doi.org/10.1093/molbev/msy075 |
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author | Ausiannikava, Darya Mitchell, Laura Marriott, Hannah Smith, Victoria Hawkins, Michelle Makarova, Kira S Koonin, Eugene V Nieduszynski, Conrad A Allers, Thorsten |
author_facet | Ausiannikava, Darya Mitchell, Laura Marriott, Hannah Smith, Victoria Hawkins, Michelle Makarova, Kira S Koonin, Eugene V Nieduszynski, Conrad A Allers, Thorsten |
author_sort | Ausiannikava, Darya |
collection | PubMed |
description | The common ancestry of archaea and eukaryotes is evident in their genome architecture. All eukaryotic and several archaeal genomes consist of multiple chromosomes, each replicated from multiple origins. Three scenarios have been proposed for the evolution of this genome architecture: 1) mutational diversification of a multi-copy chromosome; 2) capture of a new chromosome by horizontal transfer; 3) acquisition of new origins and splitting into two replication-competent chromosomes. We report an example of the third scenario: the multi-origin chromosome of the archaeon Haloferax volcanii has split into two elements via homologous recombination. The newly generated elements are bona fide chromosomes, because each bears “chromosomal” replication origins, rRNA loci, and essential genes. The new chromosomes were stable during routine growth but additional genetic manipulation, which involves selective bottlenecks, provoked further rearrangements. To the best of our knowledge, rearrangement of a naturally evolved prokaryotic genome to generate two new chromosomes has not been described previously. |
format | Online Article Text |
id | pubmed-6063281 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-60632812018-08-08 Evolution of Genome Architecture in Archaea: Spontaneous Generation of a New Chromosome in Haloferax volcanii Ausiannikava, Darya Mitchell, Laura Marriott, Hannah Smith, Victoria Hawkins, Michelle Makarova, Kira S Koonin, Eugene V Nieduszynski, Conrad A Allers, Thorsten Mol Biol Evol Discoveries The common ancestry of archaea and eukaryotes is evident in their genome architecture. All eukaryotic and several archaeal genomes consist of multiple chromosomes, each replicated from multiple origins. Three scenarios have been proposed for the evolution of this genome architecture: 1) mutational diversification of a multi-copy chromosome; 2) capture of a new chromosome by horizontal transfer; 3) acquisition of new origins and splitting into two replication-competent chromosomes. We report an example of the third scenario: the multi-origin chromosome of the archaeon Haloferax volcanii has split into two elements via homologous recombination. The newly generated elements are bona fide chromosomes, because each bears “chromosomal” replication origins, rRNA loci, and essential genes. The new chromosomes were stable during routine growth but additional genetic manipulation, which involves selective bottlenecks, provoked further rearrangements. To the best of our knowledge, rearrangement of a naturally evolved prokaryotic genome to generate two new chromosomes has not been described previously. Oxford University Press 2018-08 2018-04-16 /pmc/articles/PMC6063281/ /pubmed/29668953 http://dx.doi.org/10.1093/molbev/msy075 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Discoveries Ausiannikava, Darya Mitchell, Laura Marriott, Hannah Smith, Victoria Hawkins, Michelle Makarova, Kira S Koonin, Eugene V Nieduszynski, Conrad A Allers, Thorsten Evolution of Genome Architecture in Archaea: Spontaneous Generation of a New Chromosome in Haloferax volcanii |
title | Evolution of Genome Architecture in Archaea: Spontaneous Generation of a New Chromosome in Haloferax volcanii |
title_full | Evolution of Genome Architecture in Archaea: Spontaneous Generation of a New Chromosome in Haloferax volcanii |
title_fullStr | Evolution of Genome Architecture in Archaea: Spontaneous Generation of a New Chromosome in Haloferax volcanii |
title_full_unstemmed | Evolution of Genome Architecture in Archaea: Spontaneous Generation of a New Chromosome in Haloferax volcanii |
title_short | Evolution of Genome Architecture in Archaea: Spontaneous Generation of a New Chromosome in Haloferax volcanii |
title_sort | evolution of genome architecture in archaea: spontaneous generation of a new chromosome in haloferax volcanii |
topic | Discoveries |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063281/ https://www.ncbi.nlm.nih.gov/pubmed/29668953 http://dx.doi.org/10.1093/molbev/msy075 |
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